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原生质体融合构建高产碱性蛋白酶工程菌 被引量:15
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作者 潘延云 张贺迎 +3 位作者 周艳芬 武金霞 王延旭 吴宝东 《应用与环境生物学报》 CAS CSCD 2002年第4期422-426,共5页
用原生质体融合的方法 ,将碱性蛋白酶生产菌 2 70 9与含有碱性蛋白酶基因克隆载体pDW2 的工程菌枯草杆菌BD10 5进行细胞融合 ,得到 1株高产碱性蛋白酶的工程菌A16 .菌落的原位杂交表明 ,该菌株携有双亲的遗传物质 .A16的表型与生长特征... 用原生质体融合的方法 ,将碱性蛋白酶生产菌 2 70 9与含有碱性蛋白酶基因克隆载体pDW2 的工程菌枯草杆菌BD10 5进行细胞融合 ,得到 1株高产碱性蛋白酶的工程菌A16 .菌落的原位杂交表明 ,该菌株携有双亲的遗传物质 .A16的表型与生长特征与 2 70 9相似 ,但同样条件下发酵酶活比 2 70 9高 5 0 %~ 10 0 % ,摇瓶发酵的产酶水平最高可达 300 0 0u/mL .A16发酵所产酶的最适pH与耐温性及发酵条件等特征均与 2 70 9相同 ,适用于工业生产 .图 3表 5参 2 展开更多
关键词 原生质体融合 高产碱性蛋白酶工程菌 地衣芽孢杆菌2709
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One new species of the genus Uenostrongylium(Coleoptera:Tenebrionidae) from China 被引量:1
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作者 Caixia YUAN Ping LI Guodong REN 《Entomotaxonomia》 CSCD 2018年第1期23-26,共4页
One new species,Uenostrongylium scaber Yuan Ren sp.nov.(China,Zhejiang),is described.Habitus and illustrations are provided,and a key to the genus Uenostrongylium is also given.
关键词 darkling beetle Stenochiini TAXONOMY KEY
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Taxonomy of the genus Luprops from China, with description of the larva of Luprops horni(Coleoptera: Tenebrionidae)
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作者 Zhonghua WEI Guodong REN 《Entomotaxonomia》 CSCD 2019年第4期299-312,共14页
The genus Luprops Hope, 1833 is distributed in Palaearctic, Oriental, Sino-Japanese and Afrotropical regions, including 3 new Chinese record species described in this paper. Eleven species of this genus are known in C... The genus Luprops Hope, 1833 is distributed in Palaearctic, Oriental, Sino-Japanese and Afrotropical regions, including 3 new Chinese record species described in this paper. Eleven species of this genus are known in China. The new Chinese record species are: L. brancuccii Schawaller, 2013, L. kaszabi Schawaller, 1997 and L. rugosissimus Kaszab, 1980. A key to the known Luprops species and its morphological details are provided. In addition, larva of Luprops horni(Gebien, 1914) is reported for the first time. 展开更多
关键词 darkling beetles Lagriinae TAXONOMY China
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A new species of Ditrigona Moore(Lepidoptera:Drepanidae) in China, with a checklist to the triangularia-group
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作者 Shan JIANG Hongxiang HAN 《Entomotaxonomia》 CSCD 2019年第2期81-88,共8页
A new species, D. tenuiata in the triangularia-group of Ditrigona Moore, 1888 is described from Sichuan, China. The female genitalia of D. uniuncusa are described for the first time. Illustrations of external features... A new species, D. tenuiata in the triangularia-group of Ditrigona Moore, 1888 is described from Sichuan, China. The female genitalia of D. uniuncusa are described for the first time. Illustrations of external features and genitalia of this new species and related species are presented. A checklist to the triangulariagroup is also provided. 展开更多
关键词 Drepanoidea Drepaninae TAXONOMY
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Two new nocturnal species of the genus Anatolica(Coleoptera:Tenebrionidae:Pimeliinae)from the deserts of northwest China
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作者 Yibin BA Guodong REN Jiliang LIU 《Entomotaxonomia》 CSCD 2021年第2期130-137,共8页
Two new species of the genus Anatolica Eschscholtz,1831,A.badainica Ba,Ren&Liu sp.nov.and A.gurbantunggutica Ba&Ren sp.nov.,are reported from the deserts of northwest China.Both are described and illustrated.T... Two new species of the genus Anatolica Eschscholtz,1831,A.badainica Ba,Ren&Liu sp.nov.and A.gurbantunggutica Ba&Ren sp.nov.,are reported from the deserts of northwest China.Both are described and illustrated.The circadian rhythms of these two new species are briefly discussed. 展开更多
关键词 darkling beetles circadian rhythm taxonomy DESERT
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Notes on jumping spiders from Xizang:three new species and two new records in China(Araneae,Salticidae)
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作者 YU Kun NI Yi ZHANG Jun-Xia 《蛛形学报》 2024年第1期8-18,共11页
Three new species of jumping spiders from the Xizang region,China are described:Evarcha darthvaderi Yu&Zhang,sp.nov.(♂♀),Orcevia qogyigyacani Yu&Zhang,sp.nov.(♀),Synagelides yadong Ni,Yu&Zhang,sp.nov.(... Three new species of jumping spiders from the Xizang region,China are described:Evarcha darthvaderi Yu&Zhang,sp.nov.(♂♀),Orcevia qogyigyacani Yu&Zhang,sp.nov.(♀),Synagelides yadong Ni,Yu&Zhang,sp.nov.(♀).Two species are newly recorded in China:Phlegra prasanna Caleb&Mathai,2015 and Ptocasius urbanii(Żabka,1981),and the female of P.prasanna is described for the first time. 展开更多
关键词 new species new record TAXONOMY China Xizang
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First description of male Phrynarachne xuxiake(Araneae,Thomisidae)from Anhui Province,China
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作者 YU Long LIN Ye-Jie 《蛛形学报》 2024年第1期43-47,共5页
The male of Phrynarachne xuxiake Lin&Li,2022 is described for the first time,based on a specimen collected from the type locality in Anhui Province,China.A morphological description and illustrations are given.
关键词 ANHUI type specimens TAXONOMY crab spider
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Quantitative Assessment of the Ecological Vulnerability of Baiyangdian Wetlands in the North China Plain
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作者 TIAN Jinghan GUO Chenchen WANG Jianhua 《Journal of Resources and Ecology》 CSCD 2021年第6期814-821,共8页
Quantitative assessment of vulnerability is a core aspect of wetland vulnerability research.Taking Baiyangdian(BYD)wetlands in the North China Plain as a study area and using the‘cause-result’model,23 representative... Quantitative assessment of vulnerability is a core aspect of wetland vulnerability research.Taking Baiyangdian(BYD)wetlands in the North China Plain as a study area and using the‘cause-result’model,23 representative indicators from natural,social,sci-tech and economic elements were selected to construct an indicator system.A weight matrix was obtained by using the entropy weight method to calculate the weight value for each indicator.Based on the membership function in the fuzzy evaluation model,the membership degrees were determined to form a fuzzy relation matrix.Finally,the ecological vulnerability was quantitatively assessed based on the comprehensive evaluation index calculated by using a composite operator to combine the entropy weight matrix with the fuzzy relation matrix.The results showed that the ecological vulnerability levels of the BYD wetlands were comprehensively evaluated as Grade Ⅱ,Grade Ⅲ,Grade Ⅳ,and Grade Ⅲ in 2010,2011-2013,2014,and 2015-2017,respectively.The ecological vulnerability of the BYD wetlands increased from low fragility in 2010 to general fragility in 2011-2013,and to high fragility in 2014,reflecting the fact that the wetland ecological condition was degenerating from 2010 to 2014.The ecological vulnerability status then turned back into general fragility during 2015-2017,indicating that the ecological situation of the BYD wetlands was starting to improve.However,the ecological status of the BYD wetlands on the whole is relatively less optimistic.The major factors affecting the ecological vulnerability of the BYD wetlands were found to be industrial smoke and dust emission,wetland water area,ammonia nitrogen,total phosphorus,rate of industrial solid wastes disposed,GDP per capita,etc.This illustrates that it is a systematic project to regulate wetland vulnerability and to protect regional ecological security,which may offer researchers and policy-makers specific clues for concrete interventions. 展开更多
关键词 ecological vulnerability fuzzy evaluation entropy weight Baiyangdian wetlands the North China Plain
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