New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle ...New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle A was doped with a dye complex which consisted of two dye molecules,fluorescein and tetramethylrhodamine.These dye molecules were first linked with avidin and biotin,respectively.They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET.Nanoparticle B was doped with fluorescein only.As a result of this combination,the nanoparticles emit double wavelengths when a single excitation wavelength is used.The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.展开更多
文摘New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET).Nanoparticle A was doped with a dye complex which consisted of two dye molecules,fluorescein and tetramethylrhodamine.These dye molecules were first linked with avidin and biotin,respectively.They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET.Nanoparticle B was doped with fluorescein only.As a result of this combination,the nanoparticles emit double wavelengths when a single excitation wavelength is used.The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.
