Aim Isolation and structural elucidation of the constituents from the dried flower buds of Magnolia biondii Pamp. Methods Column chromatography and TLC were used to isolate chemical constituents. Spectroscopic methods...Aim Isolation and structural elucidation of the constituents from the dried flower buds of Magnolia biondii Pamp. Methods Column chromatography and TLC were used to isolate chemical constituents. Spectroscopic methods were employed for structural elucidation. Results One benzo furanoid neolignan （licarin B） and two bisepoxy lignans （magnolin, fargesin） were isolated and identified. Conclusion Licarin B is the first reported benzofuranoid lignan from the family Magnoliaceae.展开更多
Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, ...Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, which were separated by HPLC under thefollowing conditions: SB-C8 column (4.6 mm x 250 mm, 5 μm) was used, the mobile phase consisted ofacetonitrileam-monium acteate (20 mmol·L^(-1) , pH 4.0) (40:60, V/V) , with a flow rate of 1 .0mL·min^(-1) , the UV detection wavelength was 380 nm, and the whole process was performed at 25℃ .Results The linearity was obtained in the range of 6.25 - 500 μg· mL^(-1) candicidin/FR-008 withthe regression equation of Y = 20 461 x + 30 748 and the correlation coefficient of 0.999 1. Theinstrument precision was 1.84% and the method precision was 3.8%. Conclusion This method isaccurate, rapid and simple; it can be used for determination of candicidin/FR-008 and relatedcomponents in fermentation broth.展开更多
文摘Aim Isolation and structural elucidation of the constituents from the dried flower buds of Magnolia biondii Pamp. Methods Column chromatography and TLC were used to isolate chemical constituents. Spectroscopic methods were employed for structural elucidation. Results One benzo furanoid neolignan （licarin B） and two bisepoxy lignans （magnolin, fargesin） were isolated and identified. Conclusion Licarin B is the first reported benzofuranoid lignan from the family Magnoliaceae.
文摘Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, which were separated by HPLC under thefollowing conditions: SB-C8 column (4.6 mm x 250 mm, 5 μm) was used, the mobile phase consisted ofacetonitrileam-monium acteate (20 mmol·L^(-1) , pH 4.0) (40:60, V/V) , with a flow rate of 1 .0mL·min^(-1) , the UV detection wavelength was 380 nm, and the whole process was performed at 25℃ .Results The linearity was obtained in the range of 6.25 - 500 μg· mL^(-1) candicidin/FR-008 withthe regression equation of Y = 20 461 x + 30 748 and the correlation coefficient of 0.999 1. Theinstrument precision was 1.84% and the method precision was 3.8%. Conclusion This method isaccurate, rapid and simple; it can be used for determination of candicidin/FR-008 and relatedcomponents in fermentation broth.
