Green fluorescent protein(GFP)gene was inserted into the pseudogene(ψ)region of genome of rabies virus rHep-Flury strain,and a recombinant rabies virus carrying GFP,designated as HEP-GFP,was rescued by reverse geneti...Green fluorescent protein(GFP)gene was inserted into the pseudogene(ψ)region of genome of rabies virus rHep-Flury strain,and a recombinant rabies virus carrying GFP,designated as HEP-GFP,was rescued by reverse genetics system.It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line.The research indicated that the pseudogene(ψ)region in the genome of rHEP-Flury strain,as an independent functional unit in the process of virus assembly,could independently carry and express exogenous genes.展开更多
文摘Green fluorescent protein(GFP)gene was inserted into the pseudogene(ψ)region of genome of rabies virus rHep-Flury strain,and a recombinant rabies virus carrying GFP,designated as HEP-GFP,was rescued by reverse genetics system.It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line.The research indicated that the pseudogene(ψ)region in the genome of rHEP-Flury strain,as an independent functional unit in the process of virus assembly,could independently carry and express exogenous genes.
