AIMS The synthetic diagnosis and analysis of multiparameter in histology is evaluated for the clinical study on diagnosis of gastric cancer(GC). METHODS The expressions of MG-3c7 anatigen and cerbB-2 receptor in speci...AIMS The synthetic diagnosis and analysis of multiparameter in histology is evaluated for the clinical study on diagnosis of gastric cancer(GC). METHODS The expressions of MG-3c7 anatigen and cerbB-2 receptor in specimens of GC were detected with the technique of ABC immunnohistochemistry.The DNA content of specimens was determined with flow cytometry(FCM).The data of multi-parameter in methodology were analysed by statistics. RESULTS The expressive rates of MG-3c7 antigen and cerbB-2 receptor were observed in gastric tublar adenocarci- noma(GTA,100% and 56%),adenocarcinoma(AC,58% and 50%),and normal gastric mucosa(NGM,20% and 33%). respectively The DNA heteroploid rate was 63%(12/19)in GTA,75%(3/4)in AC and 0%(0/8)in NGM.In Detecting GTA with FCM and ABC method,the histological parameter analysis synthetically showed that the sensitivity was 82.3% specificity 88%,expentancy 95.4% and accuracy 84%.Both methods were complementary. CONCLUSIONS The synthetic diagnosis and analysis of multi-parameter and the study on histomorphology and molecular biology may be a new way for the diagnosis on GC in histology.展开更多
文摘AIMS The synthetic diagnosis and analysis of multiparameter in histology is evaluated for the clinical study on diagnosis of gastric cancer(GC). METHODS The expressions of MG-3c7 anatigen and cerbB-2 receptor in specimens of GC were detected with the technique of ABC immunnohistochemistry.The DNA content of specimens was determined with flow cytometry(FCM).The data of multi-parameter in methodology were analysed by statistics. RESULTS The expressive rates of MG-3c7 antigen and cerbB-2 receptor were observed in gastric tublar adenocarci- noma(GTA,100% and 56%),adenocarcinoma(AC,58% and 50%),and normal gastric mucosa(NGM,20% and 33%). respectively The DNA heteroploid rate was 63%(12/19)in GTA,75%(3/4)in AC and 0%(0/8)in NGM.In Detecting GTA with FCM and ABC method,the histological parameter analysis synthetically showed that the sensitivity was 82.3% specificity 88%,expentancy 95.4% and accuracy 84%.Both methods were complementary. CONCLUSIONS The synthetic diagnosis and analysis of multi-parameter and the study on histomorphology and molecular biology may be a new way for the diagnosis on GC in histology.
