AIM:To investigate the roles of H pylori vaccine with chitosan as adjuvant in the immunological therapy of H pylori infection. METHODS:Female BALB/c mice infected H pylori were randomly divided into group A,B, C,D and...AIM:To investigate the roles of H pylori vaccine with chitosan as adjuvant in the immunological therapy of H pylori infection. METHODS:Female BALB/c mice infected H pylori were randomly divided into group A,B, C,D and E,which were treated with phosphate buffered saline （PBS）,H pylori antigen,H pylori- antigen plus chitosan solution,H pylori antigen plus chitosan particles,and H pylori antigen plus cholera toxin （CT）,respectively.The vaccine was orally given once a week for 4 weeks.At the 4th week after the last immunization,these mice were killed and gastric mucosa were embedded in paraffin,then assayed with Giemsa and HE staining.At the same time,quantitative H pylori culture was performed,and enzyme-linked im- munosorbnent assay （ELISA） was used to detect anti-H pylori IgA in saliva and gastric mucosa and anti-H pylori IgG in serum. RESULTS:In the groups with chitosan as adju- vant,the eradication of H pylori was achieved in 58.33% mice,which was in accordance with that in the group with CT as adjuvant （45.45%）,and the colonization density of H pylori in the groups with chitosan as adjuvant was significantly lower than that in the other groups （F=24.166,P<0.05-0.001）.The degrees of acute inflammation in gastric mucosal were significantly lower in mice with adjuvant than those in group A and B （H =21.873,P<0.05-0.001）;the degrees of chronic inflammation in gastric mucosal were signifi- cantly lower in the groups with adjuvant chitosan than those in the other groups （H=20.213,P<0.05-0.001）.The levels of specific anti-H pylori IgA in gastric mucosa （60.18±19.87,63.01±20.92, 61.16±22.25） and saliva （3.28±1.38,2.81±1.56, 3.03±1.52）,and specific anti-H pylori IgG （12.73±3.95,12.06±4.84,11.83±4.31） in sera in the groups with adjuvant were significantly higher than those in group A （saliva:1.19±0.63,gastric mucosa:15.56±6.24,serum:6.56±3.50） and group B （saliva:1.32±0.30,gastric mucosa:27.25±6.56,serum:7.86±4.02） （P<0.05-0.001）. CONCLUSION:H pylori vaccine with chitosan as adjuvant can eradicate or significantly reduce H pylori colonization in the gastric mucosa of mice and induce local specific humoral immune response in gastric mucosa successfully.展开更多
文摘AIM:To investigate the roles of H pylori vaccine with chitosan as adjuvant in the immunological therapy of H pylori infection. METHODS:Female BALB/c mice infected H pylori were randomly divided into group A,B, C,D and E,which were treated with phosphate buffered saline （PBS）,H pylori antigen,H pylori- antigen plus chitosan solution,H pylori antigen plus chitosan particles,and H pylori antigen plus cholera toxin （CT）,respectively.The vaccine was orally given once a week for 4 weeks.At the 4th week after the last immunization,these mice were killed and gastric mucosa were embedded in paraffin,then assayed with Giemsa and HE staining.At the same time,quantitative H pylori culture was performed,and enzyme-linked im- munosorbnent assay （ELISA） was used to detect anti-H pylori IgA in saliva and gastric mucosa and anti-H pylori IgG in serum. RESULTS:In the groups with chitosan as adju- vant,the eradication of H pylori was achieved in 58.33% mice,which was in accordance with that in the group with CT as adjuvant （45.45%）,and the colonization density of H pylori in the groups with chitosan as adjuvant was significantly lower than that in the other groups （F=24.166,P<0.05-0.001）.The degrees of acute inflammation in gastric mucosal were significantly lower in mice with adjuvant than those in group A and B （H =21.873,P<0.05-0.001）;the degrees of chronic inflammation in gastric mucosal were signifi- cantly lower in the groups with adjuvant chitosan than those in the other groups （H=20.213,P<0.05-0.001）.The levels of specific anti-H pylori IgA in gastric mucosa （60.18±19.87,63.01±20.92, 61.16±22.25） and saliva （3.28±1.38,2.81±1.56, 3.03±1.52）,and specific anti-H pylori IgG （12.73±3.95,12.06±4.84,11.83±4.31） in sera in the groups with adjuvant were significantly higher than those in group A （saliva:1.19±0.63,gastric mucosa:15.56±6.24,serum:6.56±3.50） and group B （saliva:1.32±0.30,gastric mucosa:27.25±6.56,serum:7.86±4.02） （P<0.05-0.001）. CONCLUSION:H pylori vaccine with chitosan as adjuvant can eradicate or significantly reduce H pylori colonization in the gastric mucosa of mice and induce local specific humoral immune response in gastric mucosa successfully.
