以抗旱性不同的大豆(Glycine max L.)品种豫豆24(抗旱性较强)和豫豆6号(抗旱性较弱)幼苗为材料,研究在聚乙二醇(PEG)-6000渗透胁迫条件下,幼苗根线粒体ATPase水解活性与膜上非共价结合态多胺含量的关系。结果如下:渗透胁迫条件下,抗性...以抗旱性不同的大豆(Glycine max L.)品种豫豆24(抗旱性较强)和豫豆6号(抗旱性较弱)幼苗为材料,研究在聚乙二醇(PEG)-6000渗透胁迫条件下,幼苗根线粒体ATPase水解活性与膜上非共价结合态多胺含量的关系。结果如下:渗透胁迫条件下,抗性强的豫豆24的ATPase活性下降的幅度明显小于抗性弱的品种豫豆6号,而豫豆24的线粒体膜上非共价结合亚精胺(Spd)的上升幅度明显大于豫豆6号。外源Spd处理,明显促进了豫豆6号在胁迫条件下非共价结合Spd含量的增加,同时抑制了胁迫条件下ATPase活性的降低,从而提高了豫豆6号幼苗的抗性;Spd生物合成的专一性抑制剂——甲基乙二醛-双(鸟嘌呤腙)(MGBG)处理,显著抑制了豫豆24在胁迫条件下膜上非共价结合Spd的增加,同时促进其在胁迫条件下ATPase活性的降低,从而明显降低了豫豆24幼苗的抗性。结果表明,线粒体膜上非共价结合的Spd可能通过维持ATPase活性的稳定来提高大豆幼苗的抗渗透胁迫能力。展开更多
[Objective]The research aimed to probe into the mechanism of polyamine enhancing the tolerance of plants to drought stress and provide theoretical basis for application of polyamine in process of maize drought resista...[Objective]The research aimed to probe into the mechanism of polyamine enhancing the tolerance of plants to drought stress and provide theoretical basis for application of polyamine in process of maize drought resistance. [Method]With PEG-6000 simulating natural drought,the change in content of soluble protein and relative water content were investigated in seedling leaves of two maize cultivars,Nongda 108 and Yedan 13 under osmotic stress with exogenous Spd treatment. [Result]On the 7th day,leaf relative water content and the content of soluble protein decreased more significantly in leaves of Yedan 13 （drought-sensitive） than in Nongda 108 （drought-tolerant）. Exogenous Spd treatment not only obviously inhibited the decrease of leaf relative water content,but also increased the content of soluble protein. [Conclusion]Exogenous Spd treatment could enhance the tolerance of maize seedlings to osmotic stress,via improving the content of soluble protein in seedling leaves.展开更多
文摘以抗旱性不同的大豆(Glycine max L.)品种豫豆24(抗旱性较强)和豫豆6号(抗旱性较弱)幼苗为材料,研究在聚乙二醇(PEG)-6000渗透胁迫条件下,幼苗根线粒体ATPase水解活性与膜上非共价结合态多胺含量的关系。结果如下:渗透胁迫条件下,抗性强的豫豆24的ATPase活性下降的幅度明显小于抗性弱的品种豫豆6号,而豫豆24的线粒体膜上非共价结合亚精胺(Spd)的上升幅度明显大于豫豆6号。外源Spd处理,明显促进了豫豆6号在胁迫条件下非共价结合Spd含量的增加,同时抑制了胁迫条件下ATPase活性的降低,从而提高了豫豆6号幼苗的抗性;Spd生物合成的专一性抑制剂——甲基乙二醛-双(鸟嘌呤腙)(MGBG)处理,显著抑制了豫豆24在胁迫条件下膜上非共价结合Spd的增加,同时促进其在胁迫条件下ATPase活性的降低,从而明显降低了豫豆24幼苗的抗性。结果表明,线粒体膜上非共价结合的Spd可能通过维持ATPase活性的稳定来提高大豆幼苗的抗渗透胁迫能力。
基金Supported by National Natural Science Foundation of China(30771296 )Basic and Front Technology Research Program of Henan Province (082300430310)Foundation of Henan Educational Committee (2007180052)~~
文摘[Objective]The research aimed to probe into the mechanism of polyamine enhancing the tolerance of plants to drought stress and provide theoretical basis for application of polyamine in process of maize drought resistance. [Method]With PEG-6000 simulating natural drought,the change in content of soluble protein and relative water content were investigated in seedling leaves of two maize cultivars,Nongda 108 and Yedan 13 under osmotic stress with exogenous Spd treatment. [Result]On the 7th day,leaf relative water content and the content of soluble protein decreased more significantly in leaves of Yedan 13 （drought-sensitive） than in Nongda 108 （drought-tolerant）. Exogenous Spd treatment not only obviously inhibited the decrease of leaf relative water content,but also increased the content of soluble protein. [Conclusion]Exogenous Spd treatment could enhance the tolerance of maize seedlings to osmotic stress,via improving the content of soluble protein in seedling leaves.
