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口服胰岛素研究进展 被引量:7
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作者 陈敏 田浩明 《中国糖尿病杂志》 CAS CSCD 2001年第6期360-361,共2页
关键词 口服胰岛素 口服制剂 药代动力学
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Inhibitive effects of glucose and free fatty acids on proliferation of human vascular endothelial cells in vitro 被引量:1
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作者 苏进 田浩明 +1 位作者 刘瑞 梁荩忠 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第10期46-50,147,共6页
s To investigate the effects of glucose and free fatty acids (FFAs) on the proliferation and cell cycle of human vascular endothelial cells in vitro , and to examine whether the combined presence of elevated FFAs ... s To investigate the effects of glucose and free fatty acids (FFAs) on the proliferation and cell cycle of human vascular endothelial cells in vitro , and to examine whether the combined presence of elevated FFAs and glucose may cross amplify their individual injurious effects Methods Cultured human vascular endothelial cells (ECV304) were incubated with various concentrations of glucose and/or FFAs (palmitate and/or oleate) for 24-96 h Morphologic alterations were observed using a phase contrast microscope and an electron microscope Inhibition of proliferation was measured by a colorimetric 3 [4, 5 dimethyl thiazol 2 yl] 2, 5 diphenyltetrazolium bromide (MTT) assay Cell viability was determined using trypan blue exclusion Distribution of cells along phases of the cell cycle was analyzed by flow cytometry Results Glucose 15 or 30 mmol/L, palmitate (PA) 0 25 or 0 5 mmol/L, and oleate (OA) 0 5 mmol/L inhibited proliferation and accelerated death of endothelial cells in a dose and time dependent manner After treatment with elevated glucose and/or FFAs, the G 0/G 1 phase cells increased, whereas S phase cells decreased, suggesting that high glucose and/or FFAs mainly arrested endothelial cells at G 0/G 1 phase The inhibitive rates of proliferation and population of dead cells in endothelial cells incubated with glucose plus FFAs (glucose 30 mmol/L+PA 0 25 mmol/L, glucose 30 mmol/L+OA 0 5 mmol/L, glucose 30 mmol/L+PA 0 25 mmol/L+OA 0 5 mmol/L) increased more markedly than those treated with high glucose or FFAs (PA and/or OA) alone Conclusion Both high ambient glucose and FFAs can inhibit proliferation and accelerate death of endothelial cells in vitro These changes were cross amplified in the combined presence of high levels of glucose and FFAs 展开更多
关键词 glucose · free fatty acids · palmitate · oleate · endothelial cell
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