以潮霉素抗性和增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)作为筛选标记,利用地衣型真菌Cladonia metacorallifera的菌丝,成功实现了根癌农杆菌介导的遗传转化,PCR检测证明转化子中存在潮霉素抗性基因,共聚焦显微镜...以潮霉素抗性和增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)作为筛选标记,利用地衣型真菌Cladonia metacorallifera的菌丝,成功实现了根癌农杆菌介导的遗传转化,PCR检测证明转化子中存在潮霉素抗性基因,共聚焦显微镜检测到转化子菌丝能够产生绿色荧光,证明EGFP能够在trp C启动子控制下在地衣型真菌中表达。展开更多
基金韩国Development Program of the National Research foundation of korea funded by the Ministry of EducationScience and Technology of korea(Grant number:K2011-0017325)
基金Flora of the Cryptogamics of China(NSFC31093440)Beijing Academy of Science and Technology Mengya Project(2009)Beijing Natural Science Foundation(5123044)
基金Supported by the National Natural Science Foundation of China (No. 30870158)a key project of the knowledge innovation program of the Chinese Academy of Sciences (No. KSCX2-EW-Z-9)+1 种基金Korea National Research Resource Center ProgramKorean Forest Service Program (KNA 2011) through Korea National Arboretum