背景与目的 EFEMP1属于fibulin家族成员,是一种与细胞代谢密切相关的重要的细胞外基质蛋白,其在肿瘤的发生发展中的作用尚不清楚。本研究旨在探讨EFEMP1影响肺癌细胞生长和侵袭转移的生物学作用及其机制。方法 Western blot方法检测肺...背景与目的 EFEMP1属于fibulin家族成员,是一种与细胞代谢密切相关的重要的细胞外基质蛋白,其在肿瘤的发生发展中的作用尚不清楚。本研究旨在探讨EFEMP1影响肺癌细胞生长和侵袭转移的生物学作用及其机制。方法 Western blot方法检测肺癌细胞中EFEMP1表达,甲基化特异性PCR(methylation-specific PCR,MSP)方法检测EFEMP1在肺癌细胞中启动子区甲基化状态。肺癌细胞中转染EFEMP1后,检测细胞克隆形成及侵袭能力变化,并用Western blot及实时定量PCR检测MMP-7表达,Luciferase实验检测EFEMP1对基质金属蛋白酶7(matrix metalloproteinase-7,MMP-7)报告质粒的影响。结果 Western blot结果显示肺癌细胞中EFEMP1表达下降,MSP分析结果说明A549和H1299中EFEMP1启动子区存在甲基化位点,5-aza-2’-deoxycytidine处理后,EFEMP1表达升高。A549和H1299转染EFEMP1后细胞克隆形成能力以及侵袭活性明显下降,MMP-7蛋白表达下调。Luciferase实验结果显示EFEMP1可以抑制MMP-7报告质粒的表达活性。结论 EFEMP1是一种肺癌生长和侵袭的抑制因子,由于表观遗传学的改变,其在肺癌细胞中表达下降,通过上调MMP-7的表达促进肺癌细胞的侵袭转移。展开更多
目的 了解天津市儿童医院医院感染现状,为预防和控制医院感染提供依据.方法 对2005年上半年该院6101例住院患者的临床资料和2005年全年分离的医院感染病原菌进行回顾性调查,以了解感染率及致病菌耐药情况.结果 医院感染总发生率为3.47%(...目的 了解天津市儿童医院医院感染现状,为预防和控制医院感染提供依据.方法 对2005年上半年该院6101例住院患者的临床资料和2005年全年分离的医院感染病原菌进行回顾性调查,以了解感染率及致病菌耐药情况.结果 医院感染总发生率为3.47%(212/6101),其中,普通医院感染率为2.95%(180/6101),外科伤口感染率为2.66%(32/1204).在全部感染中呼吸道感染最为常见(119/212,56.1%).造成医院感染的前6位病原菌依次为大肠埃希菌、凝固酶阴性葡萄球菌、肠球菌、肺炎克雷伯菌、金黄色葡萄球菌和铜绿假单胞菌.结论 调查显示,细菌多药耐药较常见,应加强对医院感染和抗生素耐药情况的监控.
abstract:
objective to investigate the prevalence of nosocomial infections in tianjin children's hospital and to provide database for monitoring and control of nosocomial infection.methods the medical records of 6101 children admitted in the first half of 2005 and the laboratory results of isolated bacteria from clinical samples in 2005 were retrospectively investigated.results the total nosocomial infection rate was 3.47%(212/6101),in which the surgical nosocomial infection rate was 2.66%(32/1204)and 2.95%(180/6101)infections were caused by non-surgical incisions.respiratory tract was the most frequent infection site(119/212,56.1%).several opportunistic pathogens were responsible for the major nosocomial infections,they were escherichia coli,coagulase negative staphylococcus,enterococcus,klebsiella pneumonia,staphylococcus aureus and pseudolnonas aeruginosa.conclusion the pathogenic isolates for the infections show high resistance to most antibiotics.monitor and control of the incidence of nosocomial infections and resistance to antibiotics should be enforced.展开更多
文摘背景与目的 EFEMP1属于fibulin家族成员,是一种与细胞代谢密切相关的重要的细胞外基质蛋白,其在肿瘤的发生发展中的作用尚不清楚。本研究旨在探讨EFEMP1影响肺癌细胞生长和侵袭转移的生物学作用及其机制。方法 Western blot方法检测肺癌细胞中EFEMP1表达,甲基化特异性PCR(methylation-specific PCR,MSP)方法检测EFEMP1在肺癌细胞中启动子区甲基化状态。肺癌细胞中转染EFEMP1后,检测细胞克隆形成及侵袭能力变化,并用Western blot及实时定量PCR检测MMP-7表达,Luciferase实验检测EFEMP1对基质金属蛋白酶7(matrix metalloproteinase-7,MMP-7)报告质粒的影响。结果 Western blot结果显示肺癌细胞中EFEMP1表达下降,MSP分析结果说明A549和H1299中EFEMP1启动子区存在甲基化位点,5-aza-2’-deoxycytidine处理后,EFEMP1表达升高。A549和H1299转染EFEMP1后细胞克隆形成能力以及侵袭活性明显下降,MMP-7蛋白表达下调。Luciferase实验结果显示EFEMP1可以抑制MMP-7报告质粒的表达活性。结论 EFEMP1是一种肺癌生长和侵袭的抑制因子,由于表观遗传学的改变,其在肺癌细胞中表达下降,通过上调MMP-7的表达促进肺癌细胞的侵袭转移。
文摘目的 了解天津市儿童医院医院感染现状,为预防和控制医院感染提供依据.方法 对2005年上半年该院6101例住院患者的临床资料和2005年全年分离的医院感染病原菌进行回顾性调查,以了解感染率及致病菌耐药情况.结果 医院感染总发生率为3.47%(212/6101),其中,普通医院感染率为2.95%(180/6101),外科伤口感染率为2.66%(32/1204).在全部感染中呼吸道感染最为常见(119/212,56.1%).造成医院感染的前6位病原菌依次为大肠埃希菌、凝固酶阴性葡萄球菌、肠球菌、肺炎克雷伯菌、金黄色葡萄球菌和铜绿假单胞菌.结论 调查显示,细菌多药耐药较常见,应加强对医院感染和抗生素耐药情况的监控.
abstract:
objective to investigate the prevalence of nosocomial infections in tianjin children's hospital and to provide database for monitoring and control of nosocomial infection.methods the medical records of 6101 children admitted in the first half of 2005 and the laboratory results of isolated bacteria from clinical samples in 2005 were retrospectively investigated.results the total nosocomial infection rate was 3.47%(212/6101),in which the surgical nosocomial infection rate was 2.66%(32/1204)and 2.95%(180/6101)infections were caused by non-surgical incisions.respiratory tract was the most frequent infection site(119/212,56.1%).several opportunistic pathogens were responsible for the major nosocomial infections,they were escherichia coli,coagulase negative staphylococcus,enterococcus,klebsiella pneumonia,staphylococcus aureus and pseudolnonas aeruginosa.conclusion the pathogenic isolates for the infections show high resistance to most antibiotics.monitor and control of the incidence of nosocomial infections and resistance to antibiotics should be enforced.