To elucidate the potential mechanism of Wuji powder in treating polycystic ovary syndrome(PCOS),this study utilized TCMSP database to screen the active ingredients of each constituent drug in Wuji powder.Subsequently,...To elucidate the potential mechanism of Wuji powder in treating polycystic ovary syndrome(PCOS),this study utilized TCMSP database to screen the active ingredients of each constituent drug in Wuji powder.Subsequently,we predicted the target proteins associated with these active ingredients.In parallel,we employed the GeneCards database to identify genes related to PCOS and determined the intersection of drug targets and disease targets using the online tool available at http://bioinformatics.psb.ugent.be/webtools/Venn/.The shared genes were considered as the target proteins of Wuji powder in the treatment of PCOS.Utilizing the String website,we constructed a protein-protein interaction(PPI)network diagram and identified key protein modules and hub genes within the PPI network using Cytoscape 3.7.1 software.Further analysis in the DAVID database involved GO and KEGG pathway enrichment analyses of the genes within the identified key modules.Our investigation revealed a total of 63 active ingredients in Wuji powder with potential therapeutic effects on PCOS,corresponding to 266 drug targets.Intersection with PCOS-related disease targets yielded 174 shared targets.Ten key modules and ten hub genes(TNF,MMP9,AKT1,ECG,VEGFA,PTGS2,IL-6,MAPK3,STAT3,and CXCL8)were identified through network analysis.KEGG pathway enrichment analysis uncovered 58 signaling pathways,including TNF,MAPK,and PI3K-Akt signaling pathways.GO functional annotation delineated five cellular components(CC),nine molecular functions(MF),and 58 biological processes(BP).Noteworthy findings included extracellular space,enzyme binding,and drug response among CC,MF,and BP categories,respectively.These results collectively suggested that Wuji powder might exert its therapeutic effects on PCOS by modulating the TNF/PI3K/AKT signaling pathway.展开更多
2023年4月中华医学会心血管病学分会(Chinese Society of Cardiology,CSC)在《中华心血管病杂志》上发布了《盐敏感性高血压管理的中国专家共识》[1],该共识由CSC高血压学组多次组织心血管内科、高血压专科、临床流行病学专家讨论,结合...2023年4月中华医学会心血管病学分会(Chinese Society of Cardiology,CSC)在《中华心血管病杂志》上发布了《盐敏感性高血压管理的中国专家共识》[1],该共识由CSC高血压学组多次组织心血管内科、高血压专科、临床流行病学专家讨论,结合国内外相关临床研究证据和我国临床实践制定而成。该共识有几大亮点:①内容全面。展开更多
文摘To elucidate the potential mechanism of Wuji powder in treating polycystic ovary syndrome(PCOS),this study utilized TCMSP database to screen the active ingredients of each constituent drug in Wuji powder.Subsequently,we predicted the target proteins associated with these active ingredients.In parallel,we employed the GeneCards database to identify genes related to PCOS and determined the intersection of drug targets and disease targets using the online tool available at http://bioinformatics.psb.ugent.be/webtools/Venn/.The shared genes were considered as the target proteins of Wuji powder in the treatment of PCOS.Utilizing the String website,we constructed a protein-protein interaction(PPI)network diagram and identified key protein modules and hub genes within the PPI network using Cytoscape 3.7.1 software.Further analysis in the DAVID database involved GO and KEGG pathway enrichment analyses of the genes within the identified key modules.Our investigation revealed a total of 63 active ingredients in Wuji powder with potential therapeutic effects on PCOS,corresponding to 266 drug targets.Intersection with PCOS-related disease targets yielded 174 shared targets.Ten key modules and ten hub genes(TNF,MMP9,AKT1,ECG,VEGFA,PTGS2,IL-6,MAPK3,STAT3,and CXCL8)were identified through network analysis.KEGG pathway enrichment analysis uncovered 58 signaling pathways,including TNF,MAPK,and PI3K-Akt signaling pathways.GO functional annotation delineated five cellular components(CC),nine molecular functions(MF),and 58 biological processes(BP).Noteworthy findings included extracellular space,enzyme binding,and drug response among CC,MF,and BP categories,respectively.These results collectively suggested that Wuji powder might exert its therapeutic effects on PCOS by modulating the TNF/PI3K/AKT signaling pathway.
文摘2023年4月中华医学会心血管病学分会(Chinese Society of Cardiology,CSC)在《中华心血管病杂志》上发布了《盐敏感性高血压管理的中国专家共识》[1],该共识由CSC高血压学组多次组织心血管内科、高血压专科、临床流行病学专家讨论,结合国内外相关临床研究证据和我国临床实践制定而成。该共识有几大亮点:①内容全面。
