The clinical value of the detection Hanta virus antigenemia in Peripheral blood mononuclear cells (PBMC) in patients with Hemorrhagic fever with renal syndrome was evaluated. Samples from 32 cases with varying degrees...The clinical value of the detection Hanta virus antigenemia in Peripheral blood mononuclear cells (PBMC) in patients with Hemorrhagic fever with renal syndrome was evaluated. Samples from 32 cases with varying degrees of HFRS were subjected to quantitative detection for HV membrane antigen in PBMC by flow cytometry. The correlation between clinical severity and positive rates was analyzed. On the first day of onset of illness, positive PBMC rates were highly different from group to group (P<0.01), which suggested a close relationship between clinical severity and the positive rate. Dynamic observations showed that in the light and moderate groups, positive rates differed significantly from day to day. This was not the case in the severe group with no statistical significance between the 7th and 10th day(P>0.05), which implied a relatively slow rate of virus elimination. Quantitative analysis of HV antigen in PBMC by FCM is a promising method in early diagnosis, individualized therapy and in evaluating the prognosis of HFRS.展开更多
目的了解呼吸危重症医学科和重症监护病房(Intensive care unit,ICU)肺部疾病患者支气管肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)病原菌种类及其耐药性。方法回顾性分析2017年1月~2018年12月我院呼吸危重症医学科和ICU肺部疾病患...目的了解呼吸危重症医学科和重症监护病房(Intensive care unit,ICU)肺部疾病患者支气管肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)病原菌种类及其耐药性。方法回顾性分析2017年1月~2018年12月我院呼吸危重症医学科和ICU肺部疾病患者BALF分离的病原菌种类及耐药性情况。采用基质辅助激光解吸电离飞行时间质谱(Matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)对病原菌进行鉴定。结果BALF阳性检出率为17.80%(162/910),共分离培养出病原菌191株,其中真菌98株(51.31%),以念珠菌、曲霉菌和新生隐球菌为主;革兰阴性杆菌共90株(47.12%),以鲍曼不动杆菌、铜绿假单胞菌和肺炎克雷伯菌为主,多药耐药鲍曼不动杆菌对亚胺培南和美罗培南的耐药率均为58.33%,对头孢哌酮/舒巴坦和替加环素的耐药率分别为45.83%、16.67%,对哌拉西林/他唑巴坦及头孢类药物的耐药率均大于54.0%。其他革兰阴性杆菌对常用抗菌药物的耐药率不高。结论基于MALDI-TOF MS鉴定的BALF病原菌种类多样,以真菌和革兰阴性杆菌为主,其中鲍曼不动杆菌多药耐药现象严重。病原菌的准确鉴定和敏感药物使用为临床提供治疗依据。展开更多
文摘The clinical value of the detection Hanta virus antigenemia in Peripheral blood mononuclear cells (PBMC) in patients with Hemorrhagic fever with renal syndrome was evaluated. Samples from 32 cases with varying degrees of HFRS were subjected to quantitative detection for HV membrane antigen in PBMC by flow cytometry. The correlation between clinical severity and positive rates was analyzed. On the first day of onset of illness, positive PBMC rates were highly different from group to group (P<0.01), which suggested a close relationship between clinical severity and the positive rate. Dynamic observations showed that in the light and moderate groups, positive rates differed significantly from day to day. This was not the case in the severe group with no statistical significance between the 7th and 10th day(P>0.05), which implied a relatively slow rate of virus elimination. Quantitative analysis of HV antigen in PBMC by FCM is a promising method in early diagnosis, individualized therapy and in evaluating the prognosis of HFRS.
文摘目的了解呼吸危重症医学科和重症监护病房(Intensive care unit,ICU)肺部疾病患者支气管肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)病原菌种类及其耐药性。方法回顾性分析2017年1月~2018年12月我院呼吸危重症医学科和ICU肺部疾病患者BALF分离的病原菌种类及耐药性情况。采用基质辅助激光解吸电离飞行时间质谱(Matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)对病原菌进行鉴定。结果BALF阳性检出率为17.80%(162/910),共分离培养出病原菌191株,其中真菌98株(51.31%),以念珠菌、曲霉菌和新生隐球菌为主;革兰阴性杆菌共90株(47.12%),以鲍曼不动杆菌、铜绿假单胞菌和肺炎克雷伯菌为主,多药耐药鲍曼不动杆菌对亚胺培南和美罗培南的耐药率均为58.33%,对头孢哌酮/舒巴坦和替加环素的耐药率分别为45.83%、16.67%,对哌拉西林/他唑巴坦及头孢类药物的耐药率均大于54.0%。其他革兰阴性杆菌对常用抗菌药物的耐药率不高。结论基于MALDI-TOF MS鉴定的BALF病原菌种类多样,以真菌和革兰阴性杆菌为主,其中鲍曼不动杆菌多药耐药现象严重。病原菌的准确鉴定和敏感药物使用为临床提供治疗依据。
