Objective The objective of this study was to clarify the potential mechanism of quercetin against knee osteoarthritis(KOA)based on network pharmacology and molecular docking.Methods The targets of quercetin were predi...Objective The objective of this study was to clarify the potential mechanism of quercetin against knee osteoarthritis(KOA)based on network pharmacology and molecular docking.Methods The targets of quercetin were predicted by PubChem and Swiss Target Prediction databases,and the targets of KOA were obtained by DisGeNET,OMIM,and GeneCards databases.Then,the targets of quercetin and KOA were intersected to find the potential targets of quercetin against KOA.The protein–protein interaction network was constructed through the STRING database,and the core targets were screened.Gene ontology(GO)functions enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using DAVID database.The drug–target–pathway–disease network was constructed by Cytoscape software,and the molecular docking verification was performed by Vina.Results There were 49 potential targets for quercetin against KOA,including 10 core targets.GO functions enrichment analysis showed that the biological process of quercetin against KOA mainly involved the negative regulation of apoptotic process,collagen catabolic process,and extracellular matrix disassembly.KEGG pathway enrichment analysis showed that quercetin against KOA was closely related to PI3K-Akt signaling pathway,Rap 1 signaling pathway,FoxO signaling pathway,Ras signaling pathway,TNF signaling pathway,and ErbB signaling pathway.The results of molecular docking showed that the binding energies between ligand and receptors were less than−5 kcal·mol−1.Conclusions The molecular mechanism of quercetin against KOA involves many targets and pathways,which can regulate the proliferation and apoptosis of chondrocytes,degradation of extracellular matrix,and inflammatory reaction.Quercetin can stably bind to the active pockets of core target proteins,thereby exerting the effect against KOA.展开更多
Objective The resting-state functional magnetic resonance imaging(rs-f MRI)method was used to observe brain activity and its functional connection upon electroacupuncture stimulation at bilateral uterine acupoints(EX-...Objective The resting-state functional magnetic resonance imaging(rs-f MRI)method was used to observe brain activity and its functional connection upon electroacupuncture stimulation at bilateral uterine acupoints(EX-CA1),as well as to investigate the mechanism of acupuncture in the treatment of gynecological diseases.Methods Twenty-two healthy female subjects were stimulated by electroacupuncture at bilateral uterine acupoints;rs-f MRI data of the brain were acquired and standardized.Degree centrality(DC),amplitude of low-frequency fluctuation(ALFF),and regional homogeneity(ReHo)were used to analyze local spontaneous brain activity via acupuncture.An independent component analysis was used to evaluate the functional connectivity of the resting brain networks after acupuncture.Results Analytical results showed that the neural activity intensity of the precuneus lobe,orbitofrontal cortex,lingual gyrus,amygdala,and posterior central gyrus decreased after acupuncture(voxel P<0.001,cluster P<0.05).Functional connectivity analysis revealed weakened auditory and right frontal-parietal networks(voxel P<0.001,cluster P<0.05),enhanced visual network(voxel P<0.001,cluster P<0.05),and synergistic auditory network and hypothalamic-pituitary system.Conclusion Significant differences in neural activity and functional connectivity in specific brain regions were observed after acupuncture intervention at uterine acupoints;the hypothalamic-pituitary system also showed various active states in different brain regions.It is speculated that the effective mechanism of acupuncture at uterine acupoints is related to the regulation of reproductive hormones,emotional changes,and somatic sensations.Therefore,the methods used in this study could clarify the neural mechanism of uterine-point acupuncture in the treatment of gynecological diseases and may serve as a reference for other studies pertaining to acupuncture.展开更多
Clinical Practice Guideline on Acupuncture and Moxibustion:Migraine(WFAS 007.9-2023)is a clinical practice guideline officially released by the World Federation of Acupuncture-Moxibustion Societies(WFAS)on October 9,2...Clinical Practice Guideline on Acupuncture and Moxibustion:Migraine(WFAS 007.9-2023)is a clinical practice guideline officially released by the World Federation of Acupuncture-Moxibustion Societies(WFAS)on October 9,2023,and is the first international guideline on the treatment of migraine with acupuncture.This international standard was developed under the guidance of rigorous evidence-based methodology,and it contains guideline purpose,scope,applicable population,applicable settings,overview of acupuncture for migraine,guideline development process and recommendations.For promoting the understanding and application of this guideline,this article summarizes a total of 18 recommendations in order to assist clinical decisions for migraine with acupuncture.展开更多
In the present study,we aimed to assess the chemical composition changes of Semen Euphorbiae(SE)and Semen Euphorbiae Pulveratum(SEP)by UPLC-Q-TOF/MS and multivariate statistical methods.The UPLC-Q-TOF/MS method and SI...In the present study,we aimed to assess the chemical composition changes of Semen Euphorbiae(SE)and Semen Euphorbiae Pulveratum(SEP)by UPLC-Q-TOF/MS and multivariate statistical methods.The UPLC-Q-TOF/MS method and SIMCA-P software were used to analyze the changes of chemical components of SE and SEP based on PCA and PLS-DA multivariate statistical methods.A"component-target-disease"network model was constructed by Intelligent Platform for Life Sciences of traditional Chinese medicine(TCM)to predict potential related diseases.The differences of chemical composition were significant between SE and SEP.Under positive ion mode,the amounts of Euphorbia factor L2,L3,L7a,L8,L9 and lathyrol were obviously decreased after processing.Under negative ion mode,the amounts of aesculetin,bisaesculetin,ingenol and cetylic acid were increased obviously,while Euphorbia factor L1,L4 and L5 were decreased obviously after processing,and the components of euphobiasteroid,aesculetin,lathyrol and linoleic acid among the 14 differentiated compounds were closely related to the SE-related diseases through the"component-target-disease"network model.UPLC-Q-TOF/MS technology in combination with multivariate statistical methods had certain advantages in studying the complex changes of chemical composition before and after manufacturing pulveratum of SE.It provided a basis for clarifying the toxicity-attenuated mechanisms of SE manufacturing pulveratum,and laid the foundation for its further development and utilization.展开更多
基金funded by the Project of Chinese Medicine Science and Technology Development Planning in Shandong Province(2017-018)the Project of Scientific Research and Development Plan of Colleges and Universities in Shandong Province(J18KB130)the Project of the First Batch of Excellent Scientific Research and Innovation Team in Shandong University of Chinese Medicine(220316).
文摘Objective The objective of this study was to clarify the potential mechanism of quercetin against knee osteoarthritis(KOA)based on network pharmacology and molecular docking.Methods The targets of quercetin were predicted by PubChem and Swiss Target Prediction databases,and the targets of KOA were obtained by DisGeNET,OMIM,and GeneCards databases.Then,the targets of quercetin and KOA were intersected to find the potential targets of quercetin against KOA.The protein–protein interaction network was constructed through the STRING database,and the core targets were screened.Gene ontology(GO)functions enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using DAVID database.The drug–target–pathway–disease network was constructed by Cytoscape software,and the molecular docking verification was performed by Vina.Results There were 49 potential targets for quercetin against KOA,including 10 core targets.GO functions enrichment analysis showed that the biological process of quercetin against KOA mainly involved the negative regulation of apoptotic process,collagen catabolic process,and extracellular matrix disassembly.KEGG pathway enrichment analysis showed that quercetin against KOA was closely related to PI3K-Akt signaling pathway,Rap 1 signaling pathway,FoxO signaling pathway,Ras signaling pathway,TNF signaling pathway,and ErbB signaling pathway.The results of molecular docking showed that the binding energies between ligand and receptors were less than−5 kcal·mol−1.Conclusions The molecular mechanism of quercetin against KOA involves many targets and pathways,which can regulate the proliferation and apoptosis of chondrocytes,degradation of extracellular matrix,and inflammatory reaction.Quercetin can stably bind to the active pockets of core target proteins,thereby exerting the effect against KOA.
基金National Nature Science Foundation of China(61872225)Natural Science Foundation of Shandong Province(ZR2020KF013,ZR2020ZD44,ZR2019ZD04,and ZR2020QF043)+1 种基金Introduction and Cultivation Program for Young Creative Talents in Colleges and Universities of Shandong Province(2019-173)Special Fund of Qilu Health and Health Leading Talents Training Project。
文摘Objective The resting-state functional magnetic resonance imaging(rs-f MRI)method was used to observe brain activity and its functional connection upon electroacupuncture stimulation at bilateral uterine acupoints(EX-CA1),as well as to investigate the mechanism of acupuncture in the treatment of gynecological diseases.Methods Twenty-two healthy female subjects were stimulated by electroacupuncture at bilateral uterine acupoints;rs-f MRI data of the brain were acquired and standardized.Degree centrality(DC),amplitude of low-frequency fluctuation(ALFF),and regional homogeneity(ReHo)were used to analyze local spontaneous brain activity via acupuncture.An independent component analysis was used to evaluate the functional connectivity of the resting brain networks after acupuncture.Results Analytical results showed that the neural activity intensity of the precuneus lobe,orbitofrontal cortex,lingual gyrus,amygdala,and posterior central gyrus decreased after acupuncture(voxel P<0.001,cluster P<0.05).Functional connectivity analysis revealed weakened auditory and right frontal-parietal networks(voxel P<0.001,cluster P<0.05),enhanced visual network(voxel P<0.001,cluster P<0.05),and synergistic auditory network and hypothalamic-pituitary system.Conclusion Significant differences in neural activity and functional connectivity in specific brain regions were observed after acupuncture intervention at uterine acupoints;the hypothalamic-pituitary system also showed various active states in different brain regions.It is speculated that the effective mechanism of acupuncture at uterine acupoints is related to the regulation of reproductive hormones,emotional changes,and somatic sensations.Therefore,the methods used in this study could clarify the neural mechanism of uterine-point acupuncture in the treatment of gynecological diseases and may serve as a reference for other studies pertaining to acupuncture.
基金Supported by National Key Research and Development Program of China:2019YFC1712200,2019YFC1712203Independent Project of the Institute of Acupuncture and moxibustion,China Academy of Chinese Medical Sciences:ZZ202219004Science and technology innovation project of China Academy of Chinese Medical Sciences:CI2020A03510。
文摘Clinical Practice Guideline on Acupuncture and Moxibustion:Migraine(WFAS 007.9-2023)is a clinical practice guideline officially released by the World Federation of Acupuncture-Moxibustion Societies(WFAS)on October 9,2023,and is the first international guideline on the treatment of migraine with acupuncture.This international standard was developed under the guidance of rigorous evidence-based methodology,and it contains guideline purpose,scope,applicable population,applicable settings,overview of acupuncture for migraine,guideline development process and recommendations.For promoting the understanding and application of this guideline,this article summarizes a total of 18 recommendations in order to assist clinical decisions for migraine with acupuncture.
基金Beijing Natural Science Foundation(Grant No.7182097)National Natural Science foundation of China(Grant No.81673597)National Key Research and Development Program of China(Grant No.2018YFE0197900)。
文摘In the present study,we aimed to assess the chemical composition changes of Semen Euphorbiae(SE)and Semen Euphorbiae Pulveratum(SEP)by UPLC-Q-TOF/MS and multivariate statistical methods.The UPLC-Q-TOF/MS method and SIMCA-P software were used to analyze the changes of chemical components of SE and SEP based on PCA and PLS-DA multivariate statistical methods.A"component-target-disease"network model was constructed by Intelligent Platform for Life Sciences of traditional Chinese medicine(TCM)to predict potential related diseases.The differences of chemical composition were significant between SE and SEP.Under positive ion mode,the amounts of Euphorbia factor L2,L3,L7a,L8,L9 and lathyrol were obviously decreased after processing.Under negative ion mode,the amounts of aesculetin,bisaesculetin,ingenol and cetylic acid were increased obviously,while Euphorbia factor L1,L4 and L5 were decreased obviously after processing,and the components of euphobiasteroid,aesculetin,lathyrol and linoleic acid among the 14 differentiated compounds were closely related to the SE-related diseases through the"component-target-disease"network model.UPLC-Q-TOF/MS technology in combination with multivariate statistical methods had certain advantages in studying the complex changes of chemical composition before and after manufacturing pulveratum of SE.It provided a basis for clarifying the toxicity-attenuated mechanisms of SE manufacturing pulveratum,and laid the foundation for its further development and utilization.
