肾型传染性支气管炎病毒的致病性研究

欧美等发达国家，由于饲养环境控制规范，传染性支气管炎（ＩＢ）仍以传统的呼吸型为主；在我国以专业户散养为主，由于禽舍温度、饲料配方、细菌性疾病的控制均不能达到最适条件，秋冬季节常出现以肾肿胀、尿酸盐结晶以至死亡为主要表现的肾型ＩＢ。本试验观察了从典型肾...

鸡痘的诊断与防治技术研究

In this experiment the Fowlpox virus (FPV) antibody of two groups of chickens were tracked for 20 weeks by indirect enzyme-linked immunosorbent assay (ELISA). A group of chickens were artificial infected. B group were the control of healthy chickens. The results indicated that the positive antibody rate of A group reached 95% in 4 weeks. The high level antibody could last 10 weeks. After being infected again the positive antibody rate reached 100% in two weeks. The positive antibody rate of serum of some convalescent chickens infected naturally was 44%, and which of three groups from some farms that were vaccinated with pox vaccine were 60%, 70% and 10% respectively. The chickens whose antibody was proved negative changed from positive with the method were ill after they had been infected artificially. However none of the chickens whose antibody were positive suffered from the virus after they were infected artificially. In addition, the neutralization test shown the antibody of FPV might be used to treat pox disease.

一种新型鸭瘟病原的分离鉴定及其特性

从2000年起．山东省的潍坊、临朐、昌乐、昌邑、沂源等饲养肉鸭集中的地区发生一种同鸭瘟症状、剖检变化相似的疾病．但该病主要侵害1月龄以下的雏鸭．成年鸭发病率较低。从自然感染该病典型病死鸭脏器中获得1株病毒，病毒粒子直径80～200nm，呈圆形．有囊膜。进一步试验鉴定，该病毒核酸类型为DNA,ELD50为10^-3.46／0．2mL，对樱桃谷鸭胚、番鸭胚、麻鸭胚及SPF鸡胚的致死率分别为100％、90％、20%和0％。该病毒对氯仿、乙醚、酸碱处理敏感，56℃ 30min能使病毒灭活，该病毒无血凝活性．不能凝集“O”型人、鸡、鸭、鹅、猪、小鼠、豚鼠、绵羊等的红细胞。血清学试验表明，该病毒与鸭肝炎病毒阳性血清、雏番鸭细小病毒阳性血清、小鹅瘟阳性血清之间无中和作用，而能部分中和鸭瘟病毒阳性血清，表明该分离株与传统鸭瘟病毒呈部分相关性。初步确定该病毒为疱疹病毒属疱疹病毒科成员。鉴于该病用传统的鸭瘟疫苗不能预防．故现暂定名为“新型鸭瘟”。

鸡传染性支气管炎病毒变异株的分离及其S1基因序列分析

本实验对山东省泰安地区某蛋鸡场产蛋下降鸡群发生的临床症状疑似鸡传染性支气管炎病例,无菌采集气管、肺脏、肾脏等组织,接种SPF鸡胚,经病毒形态观察、血凝特性试验、动物回归试验等证实,我们鉴定到一株鸡传染性支气管炎病毒(IBV);病毒中和试验表明,该病毒为一株变异毒株,使用国外针对IBV793/B血清型发表的血清型特异性引物经RTPCR方法获得了该毒株的免疫原基因S1基因,初步确定该传染性支气管炎病毒变异毒株为793/B血清型。

鸡大肠杆菌病的治疗试验

根据药敏试验结果 ,对人工发病的大肠杆菌病病鸡进行了治疗试验 ,结果表明 ,达诺沙星 ,环丙沙星对该病治疗效果较好 ,而青霉素、强力霉素效果较差。发病后及时投喂敏感药物治疗效果较好 。