目的 研究泛耐药铜绿假单胞菌的耐药机制及遗传学特征.方法 采用k-b法测定临床分离铜绿假单胞菌对14种抗菌药物的敏感性,随机选出1株泛耐药株,采用pcr法对其进行β-内酰胺酶类药物耐药相关基因(tem、shv、ctx-m-1群、ctx-m-2群、ctx-m-...目的 研究泛耐药铜绿假单胞菌的耐药机制及遗传学特征.方法 采用k-b法测定临床分离铜绿假单胞菌对14种抗菌药物的敏感性,随机选出1株泛耐药株,采用pcr法对其进行β-内酰胺酶类药物耐药相关基因(tem、shv、ctx-m-1群、ctx-m-2群、ctx-m-9群、oxa-1群、oxa-2群、oxa-10群、per、ges、veb、carb、lcr、bel、dha、imp、vim、spm、gim、sim和oprd2)和氨基糖苷类耐药基因(含氨基糖苷类修饰酶基因、16s rrna基因甲基化酶基因)以及质粒遗传标记(traa、traf)、转座子遗传标记(tnpa,tnpu、mera)和整合子遗传标记(int Ⅰ 1)检测,并对相关基因进行序列分析.结果该株铜绿假单胞菌对14种抗菌药物均不敏感.该菌株耐β-内酰胺类基因tem-1、carb-3阳性.且伴有oprd2缺失;耐氨基糖苷类基因aac(6')-Ⅱ、ant(2")-Ⅰ阳性;Ⅰ类整合子遗传标记ing Ⅰ 1阳性、转座子遗传标记mera阳性.结论 铜绿假单胞菌耐药严重,临床上需引起重视.
abstract：
objective to investigate the genetic features of pan-drag resistant pseudomonas aeruginosa. methods the susceptibilities to 14 antibacterial agents were detected in pseudomonas aerations by k-b paper diffusing method. a strain of pan-drug resistant pseudomonas aeruginosa was selected randomly, and was used to amplify genes for β-lactam antibiotic resistance (tem, shv, ctx-m-1 group,ctx-m-2 group, ctx-m-9 group, oxa-1 group, oxa-2 group, oxa-10 group, per, ges, veb,carb, lcr, bel, dha, imp, vim, spm, gim, sim and oprd2), aminoglycosidase drug resistance genes (including aminoglycosides modification enzymes gent,16s rrna methylase gene) and genetic markers of plasmid (traa and traf), integrons (tnpa, tnpu and mera), transposons Ⅰ (int Ⅰ 1 ) by pcr,and the sequences of above genes were analyzed. results the pseudomonas aeruginosa was resistant to all 14 antibacterial agents, and the following genes were positive in pcr: tem-1 and carb-3 types of genes for β-lactam antibiotic resistance (deletion of oprd2), aac (6')-Ⅱ and ant (2")-Ⅰ of aminoglycosidase resistance genes, transposons Ⅰ (int Ⅰ 1 ), and the merit of integrons. conclusions pseudomonas aeruginosa shows high resistance to most antibacterial agents, which should draw attention in clinic.展开更多
文摘目的 研究泛耐药铜绿假单胞菌的耐药机制及遗传学特征.方法 采用k-b法测定临床分离铜绿假单胞菌对14种抗菌药物的敏感性,随机选出1株泛耐药株,采用pcr法对其进行β-内酰胺酶类药物耐药相关基因(tem、shv、ctx-m-1群、ctx-m-2群、ctx-m-9群、oxa-1群、oxa-2群、oxa-10群、per、ges、veb、carb、lcr、bel、dha、imp、vim、spm、gim、sim和oprd2)和氨基糖苷类耐药基因(含氨基糖苷类修饰酶基因、16s rrna基因甲基化酶基因)以及质粒遗传标记(traa、traf)、转座子遗传标记(tnpa,tnpu、mera)和整合子遗传标记(int Ⅰ 1)检测,并对相关基因进行序列分析.结果该株铜绿假单胞菌对14种抗菌药物均不敏感.该菌株耐β-内酰胺类基因tem-1、carb-3阳性.且伴有oprd2缺失;耐氨基糖苷类基因aac(6')-Ⅱ、ant(2")-Ⅰ阳性;Ⅰ类整合子遗传标记ing Ⅰ 1阳性、转座子遗传标记mera阳性.结论 铜绿假单胞菌耐药严重,临床上需引起重视.
abstract：
objective to investigate the genetic features of pan-drag resistant pseudomonas aeruginosa. methods the susceptibilities to 14 antibacterial agents were detected in pseudomonas aerations by k-b paper diffusing method. a strain of pan-drug resistant pseudomonas aeruginosa was selected randomly, and was used to amplify genes for β-lactam antibiotic resistance (tem, shv, ctx-m-1 group,ctx-m-2 group, ctx-m-9 group, oxa-1 group, oxa-2 group, oxa-10 group, per, ges, veb,carb, lcr, bel, dha, imp, vim, spm, gim, sim and oprd2), aminoglycosidase drug resistance genes (including aminoglycosides modification enzymes gent,16s rrna methylase gene) and genetic markers of plasmid (traa and traf), integrons (tnpa, tnpu and mera), transposons Ⅰ (int Ⅰ 1 ) by pcr,and the sequences of above genes were analyzed. results the pseudomonas aeruginosa was resistant to all 14 antibacterial agents, and the following genes were positive in pcr: tem-1 and carb-3 types of genes for β-lactam antibiotic resistance (deletion of oprd2), aac (6')-Ⅱ and ant (2")-Ⅰ of aminoglycosidase resistance genes, transposons Ⅰ (int Ⅰ 1 ), and the merit of integrons. conclusions pseudomonas aeruginosa shows high resistance to most antibacterial agents, which should draw attention in clinic.
