植物脂质转运蛋白(Lipid transfer protein,LTP)是一类小分子碱性蛋白,占植物可溶性总蛋白的4%左右,目前已经在许多植物中克隆了LTP的编码基因。LTP是一种多功能的蛋白,除了被认为参与了磷脂在生物膜之间的运输外,LTP还在生物膜、角质...植物脂质转运蛋白(Lipid transfer protein,LTP)是一类小分子碱性蛋白,占植物可溶性总蛋白的4%左右,目前已经在许多植物中克隆了LTP的编码基因。LTP是一种多功能的蛋白,除了被认为参与了磷脂在生物膜之间的运输外,LTP还在生物膜、角质和脂质的形成、植物的生殖发育以及信号的转导等生物过程中发挥了重要的作用,另外LTP作为一种食物过敏原和防御蛋白引起了科研人员越来越多的兴趣。本文综述了LTP的生物学功能,对LTP在农业上的应用前景做了展望。展开更多
[Objective] The aim was to introduce a rapid DNA extraction method for PCR detection of Arabidopsis thaliana.[Method] Through the improvement of conventional DNA extraction method,a rapid Arabidopsis thaliana DNA extr...[Objective] The aim was to introduce a rapid DNA extraction method for PCR detection of Arabidopsis thaliana.[Method] Through the improvement of conventional DNA extraction method,a rapid Arabidopsis thaliana DNA extraction method was obtained.With randomly selected Arabidopsis thaliana transgenic strains and mutants as samples,the method was verified.[Result] After electrophoresis,UV absorption detection,it was found that DNA samples are complete and less pollution,and the result of PCR amplification objective fragment was good which proved DNA is suitable as a template for PCR reaction.After PCR detection,positive plants gene amplified bands were clear,without false-positive,and the test results were satisfactory.[Conclusion] The method is suitable for rapid extraction of Arabidopsis thaliana DNA and PCR detection.展开更多
文摘植物脂质转运蛋白(Lipid transfer protein,LTP)是一类小分子碱性蛋白,占植物可溶性总蛋白的4%左右,目前已经在许多植物中克隆了LTP的编码基因。LTP是一种多功能的蛋白,除了被认为参与了磷脂在生物膜之间的运输外,LTP还在生物膜、角质和脂质的形成、植物的生殖发育以及信号的转导等生物过程中发挥了重要的作用,另外LTP作为一种食物过敏原和防御蛋白引起了科研人员越来越多的兴趣。本文综述了LTP的生物学功能,对LTP在农业上的应用前景做了展望。
基金Supported by National Science and Technology Support Plan of China(2006BAD21B04)Research Foundation for the Excellent Youth Scholars of Shandong Province(2004BS02013)Youth Foundation of Shandong Academy of Agricultural Sciences (2007YQN003)~~
文摘[Objective] The aim was to introduce a rapid DNA extraction method for PCR detection of Arabidopsis thaliana.[Method] Through the improvement of conventional DNA extraction method,a rapid Arabidopsis thaliana DNA extraction method was obtained.With randomly selected Arabidopsis thaliana transgenic strains and mutants as samples,the method was verified.[Result] After electrophoresis,UV absorption detection,it was found that DNA samples are complete and less pollution,and the result of PCR amplification objective fragment was good which proved DNA is suitable as a template for PCR reaction.After PCR detection,positive plants gene amplified bands were clear,without false-positive,and the test results were satisfactory.[Conclusion] The method is suitable for rapid extraction of Arabidopsis thaliana DNA and PCR detection.