The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). T...The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.展开更多
The length, diameter, shape coefficient of fruit, the fresh weight, length, diameter, shape coefficient, weight of seed and pulp production ratio from 6 sources of Macrocarpium officinalis (Sieb. et Zucc.) Nakai were ...The length, diameter, shape coefficient of fruit, the fresh weight, length, diameter, shape coefficient, weight of seed and pulp production ratio from 6 sources of Macrocarpium officinalis (Sieb. et Zucc.) Nakai were analyzed. For all 9 characters, there were no significant differences existed within mother trees, however, there were significant differences existed among sources and trees with the coefficients of variation vary from 13.39% to 36.43%. The order of the variation degree is among sources>among mother trees>within mother trees. Drupe size and shape were closely related with those of seed, but the pulp production ratio was not related with other characters.展开更多
文摘The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.
文摘The length, diameter, shape coefficient of fruit, the fresh weight, length, diameter, shape coefficient, weight of seed and pulp production ratio from 6 sources of Macrocarpium officinalis (Sieb. et Zucc.) Nakai were analyzed. For all 9 characters, there were no significant differences existed within mother trees, however, there were significant differences existed among sources and trees with the coefficients of variation vary from 13.39% to 36.43%. The order of the variation degree is among sources>among mother trees>within mother trees. Drupe size and shape were closely related with those of seed, but the pulp production ratio was not related with other characters.
