目的系统整合关于居家安宁疗护患者及其家属移动医疗体验的质性研究,为移动医疗在居家安宁疗护患者中的应用和发展提供循证依据。方法计算机检索PubMed、web of science、PsycINFO、CINAHL、Embace、Cochrane Library、中国知网、中国...目的系统整合关于居家安宁疗护患者及其家属移动医疗体验的质性研究,为移动医疗在居家安宁疗护患者中的应用和发展提供循证依据。方法计算机检索PubMed、web of science、PsycINFO、CINAHL、Embace、Cochrane Library、中国知网、中国生物医学文献数据库、万方及维普数据库,搜集关于居家安宁疗护患者或其家属使用移动医疗体验的质性研究,检索时限从建库至2021年6月。根据澳大利亚JBI循证卫生保健中心质性研究质量评价标准对文献进行质量评价,运用Meta整合方法对研究结果进行整合。结果共纳入11篇文献,提取37个研究发现,并归纳成11个类别和3个研究整合。整合结果一:移动医疗技术能给居家安宁疗护患者带来切实的益处;整合结果二:居家安宁疗护患者及家属使用移动医疗面临的挑战;整合结果三:居家安宁疗护患者及家属使用移动医疗的长期需求。结论移动医疗为居家安宁疗护的发展提供了新的思路,其效果受到多数居家安宁疗护患者的肯定,但未来研究应注意加强移动医疗设备及技术支持工作,并丰富功能内容及参与形式,探索居家远程安宁疗护干预模式,以进一步促进移动医疗的推广及应用。展开更多
Industrial activities such as smelting emissions,mineral combustion and industrial wastewater discharge might lead to copper pollution in the environment.This kind of copper pollution has harmful effects on aquatic o ...Industrial activities such as smelting emissions,mineral combustion and industrial wastewater discharge might lead to copper pollution in the environment.This kind of copper pollution has harmful effects on aquatic o rganisms,plants and animals through direct or indirect exposure.However,the current understanding of the toxicity of copper is rather limited.Copper overload can perturb intracellular homeostasis and induce oxidative stress and e ven cell death.Recently,cuproptosis has been identified as a copper-dependent form of cell death induced by o xidative stress in mitochondria.We uncover here that zinc transporter 1(ZNT1)is an important regulator involved in cuproptosis.Firstly,we established the copper overload-induced cell death model with the overexpression of copper importer SLC31A1 in HeLa cells.Using this model,we conducted unbiased genome-wide CRISPR-Cas9 screens in cells treated with copper.Our results revealed a significant enrichment of ZNT1 gene in both library A and library B plasmids.Knocking out of ZNT1 in HeLa cells notably prevented cuproptosis.Subsequent knockout of metal transcription factor 1(MTF1)in ZNT1-deficient cells nearly abolished their ability to resist copper-induced cell death.However,overexpression of metallothionein 1X(MT1X)in the double-knockout cells could p artially restored the resistance to cuproptosis by loss of MTF1.Mechanistically,knockout of ZNT1 could promote MT1X expression by activating MTF1.As a consequence,the interaction between MT1X and copper was e nhanced,reducing the flow of copper into mitochondria and eliminating mitochondria damage.Taken together,this study reveals the important role of ZNT1 in cuproptosis and shows MTF1-MT1X axis mediated resistance to c uproptosis.Moreover,our study will help to understand the regulatory mechanism of cellular and systemic copper homeostasis under copper overload,and present insights into novel treatments for damages caused by both genetic copper overload diseases and environmental copper contamination.展开更多
文摘目的系统整合关于居家安宁疗护患者及其家属移动医疗体验的质性研究,为移动医疗在居家安宁疗护患者中的应用和发展提供循证依据。方法计算机检索PubMed、web of science、PsycINFO、CINAHL、Embace、Cochrane Library、中国知网、中国生物医学文献数据库、万方及维普数据库,搜集关于居家安宁疗护患者或其家属使用移动医疗体验的质性研究,检索时限从建库至2021年6月。根据澳大利亚JBI循证卫生保健中心质性研究质量评价标准对文献进行质量评价,运用Meta整合方法对研究结果进行整合。结果共纳入11篇文献,提取37个研究发现,并归纳成11个类别和3个研究整合。整合结果一:移动医疗技术能给居家安宁疗护患者带来切实的益处;整合结果二:居家安宁疗护患者及家属使用移动医疗面临的挑战;整合结果三:居家安宁疗护患者及家属使用移动医疗的长期需求。结论移动医疗为居家安宁疗护的发展提供了新的思路,其效果受到多数居家安宁疗护患者的肯定,但未来研究应注意加强移动医疗设备及技术支持工作,并丰富功能内容及参与形式,探索居家远程安宁疗护干预模式,以进一步促进移动医疗的推广及应用。
文摘Industrial activities such as smelting emissions,mineral combustion and industrial wastewater discharge might lead to copper pollution in the environment.This kind of copper pollution has harmful effects on aquatic o rganisms,plants and animals through direct or indirect exposure.However,the current understanding of the toxicity of copper is rather limited.Copper overload can perturb intracellular homeostasis and induce oxidative stress and e ven cell death.Recently,cuproptosis has been identified as a copper-dependent form of cell death induced by o xidative stress in mitochondria.We uncover here that zinc transporter 1(ZNT1)is an important regulator involved in cuproptosis.Firstly,we established the copper overload-induced cell death model with the overexpression of copper importer SLC31A1 in HeLa cells.Using this model,we conducted unbiased genome-wide CRISPR-Cas9 screens in cells treated with copper.Our results revealed a significant enrichment of ZNT1 gene in both library A and library B plasmids.Knocking out of ZNT1 in HeLa cells notably prevented cuproptosis.Subsequent knockout of metal transcription factor 1(MTF1)in ZNT1-deficient cells nearly abolished their ability to resist copper-induced cell death.However,overexpression of metallothionein 1X(MT1X)in the double-knockout cells could p artially restored the resistance to cuproptosis by loss of MTF1.Mechanistically,knockout of ZNT1 could promote MT1X expression by activating MTF1.As a consequence,the interaction between MT1X and copper was e nhanced,reducing the flow of copper into mitochondria and eliminating mitochondria damage.Taken together,this study reveals the important role of ZNT1 in cuproptosis and shows MTF1-MT1X axis mediated resistance to c uproptosis.Moreover,our study will help to understand the regulatory mechanism of cellular and systemic copper homeostasis under copper overload,and present insights into novel treatments for damages caused by both genetic copper overload diseases and environmental copper contamination.
