[ Objective] The aim of this study was to introduce Phosphoenolpyruvate Carboxylase （PEPCase） gene into common wheat Linyou 145. [ Method] With the material of common wheat Linyou 145, Phosphoenolpyruvate Carboxyla...[ Objective] The aim of this study was to introduce Phosphoenolpyruvate Carboxylase （PEPCase） gene into common wheat Linyou 145. [ Method] With the material of common wheat Linyou 145, Phosphoenolpyruvate Carboxylase （PEPCase） gene was introduced into wheat embryo callus by the agrobacterium-mediated transformation system, and then analyzed through successive selection with selective medium con- taing gygrornycin to detect the gene at the molecular level. [Result] The hyg-resistant plants were obtained, and GUS histochemical staining showed the leaf of resistant plants was stained dark blue. The target bands appeared in PCR analysis. [ Conclusion] Phosphoenolpyruvate Car- boxylase （PEPCase） gene has been primarily introduced into the recipient material.展开更多
文摘[ Objective] The aim of this study was to introduce Phosphoenolpyruvate Carboxylase （PEPCase） gene into common wheat Linyou 145. [ Method] With the material of common wheat Linyou 145, Phosphoenolpyruvate Carboxylase （PEPCase） gene was introduced into wheat embryo callus by the agrobacterium-mediated transformation system, and then analyzed through successive selection with selective medium con- taing gygrornycin to detect the gene at the molecular level. [Result] The hyg-resistant plants were obtained, and GUS histochemical staining showed the leaf of resistant plants was stained dark blue. The target bands appeared in PCR analysis. [ Conclusion] Phosphoenolpyruvate Car- boxylase （PEPCase） gene has been primarily introduced into the recipient material.
