[ Objective ] The paper was to explore the phylogenetic relationship among 10 species of insects in Cletus, Hygia, Acanthocoris, Coreus and Homoeo- cerus in Coreinae. [ Method] Using vertical slab PAGE, EST isozymes o...[ Objective ] The paper was to explore the phylogenetic relationship among 10 species of insects in Cletus, Hygia, Acanthocoris, Coreus and Homoeo- cerus in Coreinae. [ Method] Using vertical slab PAGE, EST isozymes of 10 species of Riptortus linearis in Coreinae were studied. [ Result] Cluster analysis showed that the difference between 5 genera of Coreinae were greater than intraspecific differences. The genus Homoeocerus had more distant relationship with the other 4 genera of Coreinae, while it had cleser relationship with Mictinae. [ Conclusion] EST isozymes of 10 species of the subfamily were clear with strong pely- morphic and good stability, which could be used for insect identification of Coreinae and phylogenetic study of species. Combined with morphological characters and variation condition of Cyt b gene sequence, it is suggested that Homoeocerus should be separated from Coreinae and classified into Mictinae.展开更多
文摘[ Objective ] The paper was to explore the phylogenetic relationship among 10 species of insects in Cletus, Hygia, Acanthocoris, Coreus and Homoeo- cerus in Coreinae. [ Method] Using vertical slab PAGE, EST isozymes of 10 species of Riptortus linearis in Coreinae were studied. [ Result] Cluster analysis showed that the difference between 5 genera of Coreinae were greater than intraspecific differences. The genus Homoeocerus had more distant relationship with the other 4 genera of Coreinae, while it had cleser relationship with Mictinae. [ Conclusion] EST isozymes of 10 species of the subfamily were clear with strong pely- morphic and good stability, which could be used for insect identification of Coreinae and phylogenetic study of species. Combined with morphological characters and variation condition of Cyt b gene sequence, it is suggested that Homoeocerus should be separated from Coreinae and classified into Mictinae.