丙型肝炎患者血清hsa-miR-17-5p和hsa-miR-223-3p表达及意义

目的探讨丙型肝炎(丙肝)患者血清hsa-mi R-17-5p和hsa-mi R-223-3p的表达情况及意义。方法采用实时荧光定量PCR检测30例丙肝患者和32例体检健康者血清hsa-mi R-17-5p和hsa-mi R-223-3p的表达水平。结果丙肝组血清hsa-mi R-17-5p相对表达量[1.96(1.27,2.82)]比体检健康组[1.29(1.01,1.52)]显著升高(Z=2.94,P<0.05),而丙肝组血清hsami R-223-3p相对表达量[0.29(0.22,0.39)]比体检健康组[0.56(0.45,0.64)]显著降低(Z=4.21,P<0.05)。体检健康组hsa-mi R-17-5p表达水平与丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活性均无相关性(r分别为0.316和0.073,P>0.05);hsa-mi R-223-3p表达水平与ALT和AST活性亦无相关性(r分别为0.063和0.121,P>0.05)。而丙肝组hsa-mi R-17-5p表达水平与ALT和AST活性存在正相关(r分别为0.985和0.972,P<0.05);hsa-mi R-223-3p表达水平与ALT和AST活性存在负相关(r分别为-0.446和-0.450,P<0.05)。结论丙肝患者血清hsa-mi R-17-5p和hsa-mi R-223-3p表达失调,hsa-mi R-17-5p有望成为判断丙肝患者肝损伤的潜在分子标志物。