Objective This study aimed to analyze the mechanism of action of the Pingyang Jiangya Formula(平阳降压方,PYJYF)in treating hypertension,based on network pharmacology,and to verify the subsequent predictions through an...Objective This study aimed to analyze the mechanism of action of the Pingyang Jiangya Formula(平阳降压方,PYJYF)in treating hypertension,based on network pharmacology,and to verify the subsequent predictions through animal experiments.Methods The active components and related target genes of PYJYF were screened using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine(BATMAN-TCM),Encyclopedia of Traditional Chinese Medicine(ETCM),and Drug Bank databases and available literature.The hypertension target genes were screened based on Therapeutic Target Database(TTD),GeneCards,Online Mendelian Inheritance in Man(OMIM),UniProt,and relevant literature.The component-disease-target network intersection target genes were inputted into the STRING database,and the key target genes were selected according to the degree algorithm.Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed to explore the multitarget mechanism of action and molecular regulatory network of PYJYF in the treatment of hypertension.To verify this prediction,we used PYJYF to intervene in spontaneously hypertensive rats(SHRs)and Wistar–Kyoto rats(WKY)as normal control,and the noninvasive tail artery manometry method was used to measure systolic blood pressure(SBP)in the rat tail before PYJYF intervention.After drug intervention,the SBP of each group rats were measured and compared every week.Enzyme-linked immunosorbent assay(ELISA)was used to test plasma renin,angiotensin II(Ang II),and aldosterone(Ald)levels,and hematoxylin-eosin(HE)staining was used to observe pathological damage to the renal vessels in each group of rats.Western blot and reverse transcription real-time quantitative PCR(RT-PCR)were used to detect the protein and mRNA expression levels of PI3 K,AKT1,BAX,and Bcl-2,respectively.Results A total of 4123 hypertension targets were obtained from related databases.From the TCMSP and chemical databases,78 active components of PYJYF and the corresponding 401 drug targets were retrieved.Data analysis revealed that 208 drug targets directly interacted with the hypertension targets in PYJYF.The 10 targets most closely related to hypertension target proteins in PYJYF were directly retrieved from relevant databases.GO analysis revealed that 10 direct target proteins were involved in all aspects of the antihypertensive effects of PYJYF,as well as molecular biological processes,such as the regulation of blood pressure,renin-angiotensin-aldosterone system(RAAS),angiotensin-mediated ligand reactions,and biological stimulation of cardiomyocyte apoptosis.KEGG pathway enrichment analysis revealed that PYJYF directly affected 20 signaling pathways associated with hypertension.In animal experiments,PYJYF reduced the protein and m RNA levels of PI3 K,Akt,and Bax and upregulated the expression of the protein and m RNA levels of Bcl-2,reduced plasma renin,Ang II,and Ald levels,improved the hyperactivity of RAAS,and significantly reduced SBP in SHRs.Conclusion PYJYF is effective for hypertension therapy that acts through multiple compounds and targets.The possible underlying molecular mechanism includes regulating the PI3 K/Akt signaling pathway to suppress RAAS,increasing the ratio of Bcl-2/Bax proteins,and inhibiting apoptosis,thereby mediating the repair of renal and renal vascular damage caused by hypertension.These findings warrant further research for use in clinical settings.展开更多
Objective:The objective of this study is to explore the clinical effect and safety of Zhuang medicine herbal-thread moxibustion therapy for the treatment of suboptimal health status-related insomnia through a randomiz...Objective:The objective of this study is to explore the clinical effect and safety of Zhuang medicine herbal-thread moxibustion therapy for the treatment of suboptimal health status-related insomnia through a randomized clinical trial.Methods:A total number of 60 patients with insomnia of suboptimal health status(SHS) were enrolled from the Clinic of Zhuang Medicine,Renai Branch of the First Affiliated Hospital of Guangxi University of Chinese Medicine and the Clinic of Zhuang Medicine,the First Affiliated Hospital of Guangxi University of Chinese Medicine,as well as Guangxi University of Chinese Medicine.According to the random number table method,they were randomized into a Zhuang medicine herbal-thread moxibustion therapy group(30 patients) and a western medication group(30 partients).The outcome assessors were blinded for patients allocation.In the herbal-thread moxibustion group,Zhuang medicine herbal-thread moxibustion therapy was provided once daily.In the western medication group,alprazolam tablets were prescribed for oral administration once daily.One course consisted of 10 treatments.After three consecutive courses of treatment,the score of the Pittsburgh sleep quality index(PSQI) was compared between groups,and both clinical effect and safety were assessed.Results:Sixty eligible patients were enrolled,and thirty were assigned to each group and included in the data analysis.After treatment,the score of each PSQI indicator(sleep latency,sleep duration,sleep efficiency,sleep disturbance,and daytime dysfunction) and total PSQI score were compared with those before treatment in both groups(all changes were statistically significant when P <0.05).Scores in the herbal-thread moxibustion group were superior to those in the western medication group(all values were statistically significant at P <0.05).The total effective rate was 93.3% in the herbal-thread moxibustion group and 80.0% in the western medication group(P <0.05).During treatment,no adverse reactions were reported in the herbal-thread moxibustion group.In the western medication group,three patients reported dizziness and headache,and one patient reported dry mouth,the incidence of adverse reactions was 13.3%.Conclusion:The Zhuang medicine herbal-thread moxibustion therapy is a safe and effective management strategy for the clinical symptoms of suboptimal health status-related insomnia.展开更多
基金funding support from the National Natural Science Foundation of China(No.81874464)National Major New Drug Development Project(No.2019ZX09301-103)Provincial Department of Graduate Research Innovation Project of Hunan(No.CX20190565)。
文摘Objective This study aimed to analyze the mechanism of action of the Pingyang Jiangya Formula(平阳降压方,PYJYF)in treating hypertension,based on network pharmacology,and to verify the subsequent predictions through animal experiments.Methods The active components and related target genes of PYJYF were screened using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine(BATMAN-TCM),Encyclopedia of Traditional Chinese Medicine(ETCM),and Drug Bank databases and available literature.The hypertension target genes were screened based on Therapeutic Target Database(TTD),GeneCards,Online Mendelian Inheritance in Man(OMIM),UniProt,and relevant literature.The component-disease-target network intersection target genes were inputted into the STRING database,and the key target genes were selected according to the degree algorithm.Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed to explore the multitarget mechanism of action and molecular regulatory network of PYJYF in the treatment of hypertension.To verify this prediction,we used PYJYF to intervene in spontaneously hypertensive rats(SHRs)and Wistar–Kyoto rats(WKY)as normal control,and the noninvasive tail artery manometry method was used to measure systolic blood pressure(SBP)in the rat tail before PYJYF intervention.After drug intervention,the SBP of each group rats were measured and compared every week.Enzyme-linked immunosorbent assay(ELISA)was used to test plasma renin,angiotensin II(Ang II),and aldosterone(Ald)levels,and hematoxylin-eosin(HE)staining was used to observe pathological damage to the renal vessels in each group of rats.Western blot and reverse transcription real-time quantitative PCR(RT-PCR)were used to detect the protein and mRNA expression levels of PI3 K,AKT1,BAX,and Bcl-2,respectively.Results A total of 4123 hypertension targets were obtained from related databases.From the TCMSP and chemical databases,78 active components of PYJYF and the corresponding 401 drug targets were retrieved.Data analysis revealed that 208 drug targets directly interacted with the hypertension targets in PYJYF.The 10 targets most closely related to hypertension target proteins in PYJYF were directly retrieved from relevant databases.GO analysis revealed that 10 direct target proteins were involved in all aspects of the antihypertensive effects of PYJYF,as well as molecular biological processes,such as the regulation of blood pressure,renin-angiotensin-aldosterone system(RAAS),angiotensin-mediated ligand reactions,and biological stimulation of cardiomyocyte apoptosis.KEGG pathway enrichment analysis revealed that PYJYF directly affected 20 signaling pathways associated with hypertension.In animal experiments,PYJYF reduced the protein and m RNA levels of PI3 K,Akt,and Bax and upregulated the expression of the protein and m RNA levels of Bcl-2,reduced plasma renin,Ang II,and Ald levels,improved the hyperactivity of RAAS,and significantly reduced SBP in SHRs.Conclusion PYJYF is effective for hypertension therapy that acts through multiple compounds and targets.The possible underlying molecular mechanism includes regulating the PI3 K/Akt signaling pathway to suppress RAAS,increasing the ratio of Bcl-2/Bax proteins,and inhibiting apoptosis,thereby mediating the repair of renal and renal vascular damage caused by hypertension.These findings warrant further research for use in clinical settings.
基金Supported by Funding Project of Guangxi Graduate Education Innovation Program at the Autonomous Region LevelYCBZ2020069Scientific Research Project of Guangxi University of Chinese Medicine2018QN011。
文摘Objective:The objective of this study is to explore the clinical effect and safety of Zhuang medicine herbal-thread moxibustion therapy for the treatment of suboptimal health status-related insomnia through a randomized clinical trial.Methods:A total number of 60 patients with insomnia of suboptimal health status(SHS) were enrolled from the Clinic of Zhuang Medicine,Renai Branch of the First Affiliated Hospital of Guangxi University of Chinese Medicine and the Clinic of Zhuang Medicine,the First Affiliated Hospital of Guangxi University of Chinese Medicine,as well as Guangxi University of Chinese Medicine.According to the random number table method,they were randomized into a Zhuang medicine herbal-thread moxibustion therapy group(30 patients) and a western medication group(30 partients).The outcome assessors were blinded for patients allocation.In the herbal-thread moxibustion group,Zhuang medicine herbal-thread moxibustion therapy was provided once daily.In the western medication group,alprazolam tablets were prescribed for oral administration once daily.One course consisted of 10 treatments.After three consecutive courses of treatment,the score of the Pittsburgh sleep quality index(PSQI) was compared between groups,and both clinical effect and safety were assessed.Results:Sixty eligible patients were enrolled,and thirty were assigned to each group and included in the data analysis.After treatment,the score of each PSQI indicator(sleep latency,sleep duration,sleep efficiency,sleep disturbance,and daytime dysfunction) and total PSQI score were compared with those before treatment in both groups(all changes were statistically significant when P <0.05).Scores in the herbal-thread moxibustion group were superior to those in the western medication group(all values were statistically significant at P <0.05).The total effective rate was 93.3% in the herbal-thread moxibustion group and 80.0% in the western medication group(P <0.05).During treatment,no adverse reactions were reported in the herbal-thread moxibustion group.In the western medication group,three patients reported dizziness and headache,and one patient reported dry mouth,the incidence of adverse reactions was 13.3%.Conclusion:The Zhuang medicine herbal-thread moxibustion therapy is a safe and effective management strategy for the clinical symptoms of suboptimal health status-related insomnia.
