In order to study the distribution of β-EP and gastrin immunoreactive cells in the sublingual gland, we used the Advidin Biptin-Peroxidase Complex(ABC) immunohistochemical technique to detect the expression of β-EP ...In order to study the distribution of β-EP and gastrin immunoreactive cells in the sublingual gland, we used the Advidin Biptin-Peroxidase Complex(ABC) immunohistochemical technique to detect the expression of β-EP and gastrin in rat sublingual glands. The results show that the epithelial cells of striated ducts and interlobular ducts appeared β-EP and gastrin positive in immunoreaction and the mucosus and serous acinar cells were immunoreactive negative.The positive substances were distributed in the cytoplasm with negative nuclei.These results suggest that β-EP and gastrin are expressed in the epithelial cells of ducts in the rat sublingual gland, which may play an important role in the secretion and functional regulation of the sublingual gland.展开更多
目的观察大鼠实验性胃溃疡期间前脑生长抑素免疫反应(somatostatin,SS)和 SSmRNA 神经元的变化.方法用成年♂Wistar 大鼠38只,分为实验性胃溃疡组(EUG)、盐水对照组(SCG)和正常对照组(NCG).于术后1,4,10 d 和23 d 分批取脑,石蜡切片,用 ...目的观察大鼠实验性胃溃疡期间前脑生长抑素免疫反应(somatostatin,SS)和 SSmRNA 神经元的变化.方法用成年♂Wistar 大鼠38只,分为实验性胃溃疡组(EUG)、盐水对照组(SCG)和正常对照组(NCG).于术后1,4,10 d 和23 d 分批取脑,石蜡切片,用 Stemberger 免疫组化 PAP法显示弓状核后段水平前脑 SS 神经元;用地高辛标记反义SScRNA 探针和碱性磷酸酶标记抗地高辛抗体检测系统,显示SSmRNA 神经元.结果正常大鼠 SS 神经元广泛分布于前脑各核区,溃疡组术后1 d,大脑皮质纹状皮质区 SS 神经元减少,但可见大量 SS 强阳性纤维,SS 神经元数与盐水组相比无差异;在杏仁复合体和下丘脑各核区,溃疡组 SS 神经元数增多,与盐水组、正常组相比有差异[杏仁中央核(ce):EUG 57.1±3.0 vs SCG 41.7±1.2,杏仁外侧核(1a):EUG 56.3±1.2 vs SCG 49.3±2.7,杏仁皮质后核(pco):EUG 66.7±6.7 vs SCG 44.9±3.8,杏仁基外侧核(blp):EUG 65.0±8.3 vs SCG 34.2±3.0,下丘脑弓状核(ar):EUG 92.7±8.0 vs SCG 60.9±1.5,下丘脑腹内侧核(vmh):EUG 78.7±7.3 vs SCG 47.5±1.5,P<0.01;杏仁内侧核(me):EUG 77.3±6.0 vs NCG 41.7±2.2 andEUG vsSCG 49.8±2.1,下丘脑背外侧核(dmh):EUG 93.7±4.3 vsNCG 44.7±2.0 and EUG vs SCG 61.3±0.9,ce:EUG vsNCG 35.7±4.3,1a:EUG vs NCG 11.7±4.3,pco:EUG vsNCG 35.7±1.7,blp:EUG vs NCG 29.7±0.5,ar:EUG vsNCG 30.3±1.0,vmh:EUG vs NCG 30.7±4.3,P<0.001].术后4 d,溃疡组大脑皮质纹状皮质区、杏仁复合体和下丘脑各核区 SS 神经元密集,并在下丘脑弓状核及其周围可见较多"点样"阳性结构和串珠状 SS 阳性纤维;SS 神经元数明显增多,与盐水组、正常组相比均有差异(纹状皮质Ⅱ～Ⅲ:EUG 88.0±5.9 vs SCG 79.3±2.3.me:EUG 106.4±14.7 vs SCG 77.3±8.0 P<0.05;纹状皮质Ⅳ:EUG 83.5±3.8 vs SCG 50.7±2.0,纹状皮质Ⅴ～Ⅵ:EUG 79.5±4.4 vs SCG 70.0±1.3,ce:EUG 106.9±14.7 vs SCG 62.0±11.0,1a:EUG 83.5±6.6 vs SCG 60.0±5.3.pco:EUG 112.8±11.4 vs SCG 85.7±4.5,blp:EUG 90.1±8.4 vs SCG 60.0±3.3,ar:EUG152.5±16.1 vs SCG 95.3±5.7,wnh:EUG 130.1±10.8 vsSCG 84.7±18.0,P<0.01;dmh:EUG 145.3±8.0 vs SCG114.0±4.7,纹状皮质Ⅱ～Ⅲ:EUG 88.0±5.9 vs NCG 40.7±1.3,,纹状皮质Ⅳ:EUG vs NCG20.4±1.3,纹状皮质Ⅴ～Ⅵ:EUG vs NCG 32.3±1.7,me:EUG vs NCG,ce:EUG vsNCG,la:EUG vs NCG,pco:EUG vs NCG,blp:EUG vsNOD,ar:EUG vs NCG,vmh:EUG vs NCG,dmh:EUG vsNCG,P<0.001).术后10 d 和23 d,各核区 SS 神经元数仍多于盐水组和正常组,术后4 d,SSmRNA 神经元数量于溃疡组大脑皮质(EUG 239±7,SCG 122±6)、杏仁复合体(EUG 637±53,SCG 256±16)和下丘脑弓状核(EUG 96±18,SCG 51±5)明显多于盐水组(P<0.001).结论实验性胃溃疡大鼠前脑 SS-ir 和 SSmRNA 神经元可能参与了胃溃疡自愈过程的调节.展开更多
文摘In order to study the distribution of β-EP and gastrin immunoreactive cells in the sublingual gland, we used the Advidin Biptin-Peroxidase Complex(ABC) immunohistochemical technique to detect the expression of β-EP and gastrin in rat sublingual glands. The results show that the epithelial cells of striated ducts and interlobular ducts appeared β-EP and gastrin positive in immunoreaction and the mucosus and serous acinar cells were immunoreactive negative.The positive substances were distributed in the cytoplasm with negative nuclei.These results suggest that β-EP and gastrin are expressed in the epithelial cells of ducts in the rat sublingual gland, which may play an important role in the secretion and functional regulation of the sublingual gland.
文摘目的观察大鼠实验性胃溃疡期间前脑生长抑素免疫反应(somatostatin,SS)和 SSmRNA 神经元的变化.方法用成年♂Wistar 大鼠38只,分为实验性胃溃疡组(EUG)、盐水对照组(SCG)和正常对照组(NCG).于术后1,4,10 d 和23 d 分批取脑,石蜡切片,用 Stemberger 免疫组化 PAP法显示弓状核后段水平前脑 SS 神经元;用地高辛标记反义SScRNA 探针和碱性磷酸酶标记抗地高辛抗体检测系统,显示SSmRNA 神经元.结果正常大鼠 SS 神经元广泛分布于前脑各核区,溃疡组术后1 d,大脑皮质纹状皮质区 SS 神经元减少,但可见大量 SS 强阳性纤维,SS 神经元数与盐水组相比无差异;在杏仁复合体和下丘脑各核区,溃疡组 SS 神经元数增多,与盐水组、正常组相比有差异[杏仁中央核(ce):EUG 57.1±3.0 vs SCG 41.7±1.2,杏仁外侧核(1a):EUG 56.3±1.2 vs SCG 49.3±2.7,杏仁皮质后核(pco):EUG 66.7±6.7 vs SCG 44.9±3.8,杏仁基外侧核(blp):EUG 65.0±8.3 vs SCG 34.2±3.0,下丘脑弓状核(ar):EUG 92.7±8.0 vs SCG 60.9±1.5,下丘脑腹内侧核(vmh):EUG 78.7±7.3 vs SCG 47.5±1.5,P<0.01;杏仁内侧核(me):EUG 77.3±6.0 vs NCG 41.7±2.2 andEUG vsSCG 49.8±2.1,下丘脑背外侧核(dmh):EUG 93.7±4.3 vsNCG 44.7±2.0 and EUG vs SCG 61.3±0.9,ce:EUG vsNCG 35.7±4.3,1a:EUG vs NCG 11.7±4.3,pco:EUG vsNCG 35.7±1.7,blp:EUG vs NCG 29.7±0.5,ar:EUG vsNCG 30.3±1.0,vmh:EUG vs NCG 30.7±4.3,P<0.001].术后4 d,溃疡组大脑皮质纹状皮质区、杏仁复合体和下丘脑各核区 SS 神经元密集,并在下丘脑弓状核及其周围可见较多"点样"阳性结构和串珠状 SS 阳性纤维;SS 神经元数明显增多,与盐水组、正常组相比均有差异(纹状皮质Ⅱ～Ⅲ:EUG 88.0±5.9 vs SCG 79.3±2.3.me:EUG 106.4±14.7 vs SCG 77.3±8.0 P<0.05;纹状皮质Ⅳ:EUG 83.5±3.8 vs SCG 50.7±2.0,纹状皮质Ⅴ～Ⅵ:EUG 79.5±4.4 vs SCG 70.0±1.3,ce:EUG 106.9±14.7 vs SCG 62.0±11.0,1a:EUG 83.5±6.6 vs SCG 60.0±5.3.pco:EUG 112.8±11.4 vs SCG 85.7±4.5,blp:EUG 90.1±8.4 vs SCG 60.0±3.3,ar:EUG152.5±16.1 vs SCG 95.3±5.7,wnh:EUG 130.1±10.8 vsSCG 84.7±18.0,P<0.01;dmh:EUG 145.3±8.0 vs SCG114.0±4.7,纹状皮质Ⅱ～Ⅲ:EUG 88.0±5.9 vs NCG 40.7±1.3,,纹状皮质Ⅳ:EUG vs NCG20.4±1.3,纹状皮质Ⅴ～Ⅵ:EUG vs NCG 32.3±1.7,me:EUG vs NCG,ce:EUG vsNCG,la:EUG vs NCG,pco:EUG vs NCG,blp:EUG vsNOD,ar:EUG vs NCG,vmh:EUG vs NCG,dmh:EUG vsNCG,P<0.001).术后10 d 和23 d,各核区 SS 神经元数仍多于盐水组和正常组,术后4 d,SSmRNA 神经元数量于溃疡组大脑皮质(EUG 239±7,SCG 122±6)、杏仁复合体(EUG 637±53,SCG 256±16)和下丘脑弓状核(EUG 96±18,SCG 51±5)明显多于盐水组(P<0.001).结论实验性胃溃疡大鼠前脑 SS-ir 和 SSmRNA 神经元可能参与了胃溃疡自愈过程的调节.
