以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结...以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.展开更多
目的:分析2014~2018年成都市双流区恶性肿瘤死亡情况及其对居民寿命的影响,为该区恶性肿瘤防控提供科学依据。方法:分析2014~2018年成都市双流区肿瘤登记处收集的恶性肿瘤死亡资料,计算粗死亡率、标化死亡率、潜在减寿年数(potential ye...目的:分析2014~2018年成都市双流区恶性肿瘤死亡情况及其对居民寿命的影响,为该区恶性肿瘤防控提供科学依据。方法:分析2014~2018年成都市双流区肿瘤登记处收集的恶性肿瘤死亡资料,计算粗死亡率、标化死亡率、潜在减寿年数(potential years of life lost,PYLL)、潜在减寿率(potential years of life lost rate,PYLLR)、去死因期望寿命。结果:2014~2018年双流区恶性肿瘤粗死亡率、中标死亡率、世标死亡率分别为173.84/10万、123.30/10万、121.62/10万,男性死亡率高于女性,差异有统计学意义(P<0.05)。死亡前五位癌种分别是肺癌、食管癌、肝癌、结直肠癌和胃癌。2014~2018年间恶性肿瘤所致的潜在减寿年数为48259人年(男32020.5人年,女16238.5人年),PYLLR为17.20‰(男22.20‰,女11.90‰)。男性恶性肿瘤PYLL高于女性,差异有统计学意义(Z=15.000,P<0.05)。潜在减寿年数前五位的恶性肿瘤分别为肺癌、肝癌、食管癌、结直肠癌和胃癌。2014~2018年间该区居民期望寿命为80.02岁,男性77.12岁,女性81.91岁。去除恶性肿瘤死因后期望寿命增值为3.74岁。结论:双流区恶性肿瘤疾病负担较重,对居民期望寿命影响严重。应重点防治肺癌、食管癌、肝癌、结直肠癌、胃癌和女性乳腺癌。展开更多
文摘以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.
文摘目的:分析2014~2018年成都市双流区恶性肿瘤死亡情况及其对居民寿命的影响,为该区恶性肿瘤防控提供科学依据。方法:分析2014~2018年成都市双流区肿瘤登记处收集的恶性肿瘤死亡资料,计算粗死亡率、标化死亡率、潜在减寿年数(potential years of life lost,PYLL)、潜在减寿率(potential years of life lost rate,PYLLR)、去死因期望寿命。结果:2014~2018年双流区恶性肿瘤粗死亡率、中标死亡率、世标死亡率分别为173.84/10万、123.30/10万、121.62/10万,男性死亡率高于女性,差异有统计学意义(P<0.05)。死亡前五位癌种分别是肺癌、食管癌、肝癌、结直肠癌和胃癌。2014~2018年间恶性肿瘤所致的潜在减寿年数为48259人年(男32020.5人年,女16238.5人年),PYLLR为17.20‰(男22.20‰,女11.90‰)。男性恶性肿瘤PYLL高于女性,差异有统计学意义(Z=15.000,P<0.05)。潜在减寿年数前五位的恶性肿瘤分别为肺癌、肝癌、食管癌、结直肠癌和胃癌。2014~2018年间该区居民期望寿命为80.02岁,男性77.12岁,女性81.91岁。去除恶性肿瘤死因后期望寿命增值为3.74岁。结论:双流区恶性肿瘤疾病负担较重,对居民期望寿命影响严重。应重点防治肺癌、食管癌、肝癌、结直肠癌、胃癌和女性乳腺癌。
