Bi-2-naphthol(BINOL) enantiomers were baseline resolved on HPLC network-polymeric chiral stationary phase (Kromasil CHI-DMB, based on O,O′-di(3,5-dimethylbenzoyl)N,N′-diallyl-L-tartaric diamide). The effects of the ...Bi-2-naphthol(BINOL) enantiomers were baseline resolved on HPLC network-polymeric chiral stationary phase (Kromasil CHI-DMB, based on O,O′-di(3,5-dimethylbenzoyl)N,N′-diallyl-L-tartaric diamide). The effects of the column temperature, the type and the concentration of the polar alcohol modifier in the binary mobile phase on the chiral resolution were examined. The separation value was 1.191 when V(hexane)∶V(2-propanol)=95∶5 was used as mobile phase at a flow rate of 1.0 mL/min at 25 ℃ with retention time being within 14 minutes. The mechanism of the chiral recognition was discussed with the calculated thermodynamic parameters. It is suggested that hydrogen bonding interaction between hydroxyl group of the solute and the CSP play important roles in chiral recognition. The chiral resolution is enthalpy-entropy driven and the enthalpy contribution is greater. 1,1′-Bi-2-naphthyl di-p-toluenesulfonate, 1,1′-bi-2-naphthyl diacetate and 1,1′-bi-2-naphthyl dicinnamate could not be resolved at experiment conditions used.展开更多
目的:优化枇杷花总三萜提取工艺。方法:以总三萜为考察指标,通过单因素及L9(34)正交试验优选枇杷花总三萜的提取工艺。结果:确定在40℃、超声功率800 W、超声间歇时间为10/3 s条件下,以1∶12的料液比提取2次,每次15 m in为最佳提取条件...目的:优化枇杷花总三萜提取工艺。方法:以总三萜为考察指标,通过单因素及L9(34)正交试验优选枇杷花总三萜的提取工艺。结果:确定在40℃、超声功率800 W、超声间歇时间为10/3 s条件下,以1∶12的料液比提取2次,每次15 m in为最佳提取条件。结论:本实验采用的方法和指标适合于枇杷花总三萜的提取,为将其开发成为现代中药成方制剂提供了科学依据。展开更多
文摘Bi-2-naphthol(BINOL) enantiomers were baseline resolved on HPLC network-polymeric chiral stationary phase (Kromasil CHI-DMB, based on O,O′-di(3,5-dimethylbenzoyl)N,N′-diallyl-L-tartaric diamide). The effects of the column temperature, the type and the concentration of the polar alcohol modifier in the binary mobile phase on the chiral resolution were examined. The separation value was 1.191 when V(hexane)∶V(2-propanol)=95∶5 was used as mobile phase at a flow rate of 1.0 mL/min at 25 ℃ with retention time being within 14 minutes. The mechanism of the chiral recognition was discussed with the calculated thermodynamic parameters. It is suggested that hydrogen bonding interaction between hydroxyl group of the solute and the CSP play important roles in chiral recognition. The chiral resolution is enthalpy-entropy driven and the enthalpy contribution is greater. 1,1′-Bi-2-naphthyl di-p-toluenesulfonate, 1,1′-bi-2-naphthyl diacetate and 1,1′-bi-2-naphthyl dicinnamate could not be resolved at experiment conditions used.
文摘目的:优化枇杷花总三萜提取工艺。方法:以总三萜为考察指标,通过单因素及L9(34)正交试验优选枇杷花总三萜的提取工艺。结果:确定在40℃、超声功率800 W、超声间歇时间为10/3 s条件下,以1∶12的料液比提取2次,每次15 m in为最佳提取条件。结论:本实验采用的方法和指标适合于枇杷花总三萜的提取,为将其开发成为现代中药成方制剂提供了科学依据。
