负载外泌体水凝胶修饰3D打印支架构建血管化的气道替代物

背景:对于长段气管缺损的替代治疗,尽管近年来组织工程研究取得了一定的进展,但仍然不尽完善,其中最大的难点就是不能快速实现气管替代物的血运重建。目的:初步探索经负载外泌体水凝胶修饰的聚己内酯支架构建可快速血管化气管替代物的潜力。方法:提取SD大鼠骨髓间充质干细胞来源外泌体,制备甲基丙烯酰化透明质酸溶液,将外泌体溶液与甲基丙烯酰化透明质酸溶液按照体积比1∶1混合,紫外光照射5 min制备负载外泌体的甲基丙烯酰化透明质酸水凝胶。检测未负载外泌体水凝胶的降解情况,负载外泌体水凝胶外泌体的控制释放情况。采用3D打印法制备聚己内酯支架,将单纯的甲基丙烯酰化透明质酸溶液与负载外泌体的甲基丙烯酰化透明质酸水溶液分别滴加至支架表面,紫外光照射后获得水凝胶修饰的支架与外泌体修饰的支架。将30只SD大鼠采用随机数字表法分为3组,每组10只,分别于皮下包埋单纯的支架、水凝胶修饰的支架与外泌体修饰的支架,术后30 d取出各组支架及周围组织,通过苏木精-伊红及Masson染色观察新生血管生成情况,免疫荧光检测CD31的表达。结果与结论:①随着时间的推移,水凝胶逐渐降解,并且包封于水凝胶中的外泌体逐渐释放,可持续释放至30 d以上,并且外泌体的释放速率快于水凝胶自身的降解速率,浸泡30 d时仍有近20%的外泌体未被释放;②扫描电镜下可见,单纯的聚己内酯支架表面粗糙,经水凝胶修饰后,支架孔隙之间覆盖一层凝胶物质,支架表面变得光滑、致密;③大鼠皮下包埋30 d后,苏木精-伊红及Masson染色显示,与水凝胶修饰支架组相比,外泌体修饰支架组支架内部可见更多的新生血管形成,两组支架表面的水凝胶未完全降解;免疫荧光染色显示,外泌体修饰支架组CD31表达高于水凝胶修饰支架组(P<0.0001);④结果表明,甲基丙烯酰化透明质酸水凝胶可以作为外泌体的控释载体,经负载外泌体甲基丙烯酰化透明质酸水凝胶修饰后的3D打印聚己内酯支架,具有良好的生物相容性,且具有促进新生血管形成的潜力。

A novel preparation of Panax japonicas with high bioequivalence of ginsenoside Rg3

In the present study,we aimed to prepare Panax japonicus tablets and carry out quality inspection.Panax japonicus tablets were prepared by ultrafine pulverization-wet granulation,and quality inspection was carried out according to Pharmacopoeia regulations.The plasma concentration of animals with self-made Panax japonicus tablets or ginsenoside Rg3 in single-dose intragastric administration was determined by high-performance liquid chromatography(HPLC).The pharmacokinetic parameters and relative bioavailability were calculated by DAS 2.0 software.The quality inspection of self-made Panax japonicus tablets met the requirements of Chinese Pharmacopoeia(2015 edition),and this preparation had high bioequivalence of ginsenoside Rg3.The preparation of Panax japonicus tablets was reasonable and highly qualified.Moreover,this new Panax japonicus preparation showed better profiles in oral absorption and utilization.This study provided evidence for the industrial production and clinical application of Panax japonicus tablets.

Porcine placental peptides improve neuroblast proliferation and differentiation via enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model

Aging affects the nervous system as well as other organs.In our study,we aimed to study the pharmacological effects and mechanism of porcine placental peptides(PPP)in aging process,and to observe the changes of neuroblast proliferation and differentiation as well as partial gene expression in hippocampus of D-galactose-induced aged mouse.Based on the analysis of experimental results,it was confirmed that PPP significantly improved neurobalst proliferation and differentiation in the mouse hippocampal DG by ki-67 and DCX immunohistochemistry.This result showed that PPP had anti-aging effects on D-galactoseinduced aging mouse model.Moreover,we observed up-regulated expressions of BDNF and TrkB proteins and down-regulated expressions of Caspase 3,8,and 9 proteins in the PPP-treated mouse hippocampus.Therefore,our results showed that PPP obviously improved neuroblast proliferation and differentiation,and its anti-aging effect might berelated to down-regulation of apoptosis-related proteins,including Caspase 3,8,and 9,via BDNF/TrkB pathway.Our findings provided valuable evidence for its applications in the health and medicine sectors.