本研究利用改进的溶胶-凝胶工艺合成了介孔硅基干凝胶。通过差热-热重(DTA-TG)、X-射线衍射(XRD)、红外光谱(FTIR)和N2等温吸附-脱附测试分析,以及扫描电镜(SEM)、透射电镜(TEM)观察,表征了硅基干凝胶的热效应、相态、结构、形貌特征,...本研究利用改进的溶胶-凝胶工艺合成了介孔硅基干凝胶。通过差热-热重(DTA-TG)、X-射线衍射(XRD)、红外光谱(FTIR)和N2等温吸附-脱附测试分析,以及扫描电镜(SEM)、透射电镜(TEM)观察,表征了硅基干凝胶的热效应、相态、结构、形貌特征,并对合成的硅基干凝胶进行了体外降解与细胞培养实验研究。测试结果表明:合成的硅基干凝胶为平均孔径3nm左右、最大比表面积901m2·g-1的介孔结构。体外模拟体液(Simulated body fluid,SBF)降解结果显示,低温处理的材料在42d内完全降解,直线拟合符合一级反应动力学方程。通过添加钙磷成分或高温煅烧处理可以调控干凝胶的降解速率,使硅基干凝胶的降解呈现直线式匀速降解的特征。体外细胞培养表明:硅基干凝胶可促进C2C12细胞(C3H小鼠肌原细胞)的分化和繁殖,证明材料的生物相容性良好。展开更多
A novel biodegradable bone repair biomaterial of bone-like carbonated apatite with porous structurewas prepared by using self-hardening calcium phosphate cement. Cell culture, degradation in simulated body liq-uid (SB...A novel biodegradable bone repair biomaterial of bone-like carbonated apatite with porous structurewas prepared by using self-hardening calcium phosphate cement. Cell culture, degradation in simulated body liq-uid (SBF) and as a carrier for bone morphogenic protein (BMP) controllable releasing experiments were performedto evaluate the biocompatibility, degradation and BMP carrier properties of the porous scaffold. The results revealthat the degradation property of the carbonated apatite is better than hydroxyapatite, the more the content of CO32-in apatite, the faster the degradation of the materials, the cell could attach, proliferate and differentiate on theporous scaffold, indicating that the bone like apatites not only have excellent biocompatibility but are alsobiodegradable and can be used as carriers for BMP controlling release.展开更多
文摘本研究利用改进的溶胶-凝胶工艺合成了介孔硅基干凝胶。通过差热-热重(DTA-TG)、X-射线衍射(XRD)、红外光谱(FTIR)和N2等温吸附-脱附测试分析,以及扫描电镜(SEM)、透射电镜(TEM)观察,表征了硅基干凝胶的热效应、相态、结构、形貌特征,并对合成的硅基干凝胶进行了体外降解与细胞培养实验研究。测试结果表明:合成的硅基干凝胶为平均孔径3nm左右、最大比表面积901m2·g-1的介孔结构。体外模拟体液(Simulated body fluid,SBF)降解结果显示,低温处理的材料在42d内完全降解,直线拟合符合一级反应动力学方程。通过添加钙磷成分或高温煅烧处理可以调控干凝胶的降解速率,使硅基干凝胶的降解呈现直线式匀速降解的特征。体外细胞培养表明:硅基干凝胶可促进C2C12细胞(C3H小鼠肌原细胞)的分化和繁殖,证明材料的生物相容性良好。
文摘A novel biodegradable bone repair biomaterial of bone-like carbonated apatite with porous structurewas prepared by using self-hardening calcium phosphate cement. Cell culture, degradation in simulated body liq-uid (SBF) and as a carrier for bone morphogenic protein (BMP) controllable releasing experiments were performedto evaluate the biocompatibility, degradation and BMP carrier properties of the porous scaffold. The results revealthat the degradation property of the carbonated apatite is better than hydroxyapatite, the more the content of CO32-in apatite, the faster the degradation of the materials, the cell could attach, proliferate and differentiate on theporous scaffold, indicating that the bone like apatites not only have excellent biocompatibility but are alsobiodegradable and can be used as carriers for BMP controlling release.
