Based on the analysis of metabolic pathway Streptococcous zooepidemicus for hyaluronic acid (HA) synthesis,nucleotide,especially uracil,was considered to be important to cell growth and metabolism.When 0.005 g·L ...Based on the analysis of metabolic pathway Streptococcous zooepidemicus for hyaluronic acid (HA) synthesis,nucleotide,especially uracil,was considered to be important to cell growth and metabolism.When 0.005 g·L -1 uracil added in the media in which yeast extract as complex nitrogen source,cell growth and HA production were increased by 32% and 34% respectively.From analysis of amino acid in fermentation process,it was show that arginine(Arg)was needed for cell metabolism,and concentration of free Arg maintained at 0 g·L -1 in fermentation process,which was proposed to limit cell growth and HA production.By shake flask experiment HA concentration reached 0.510 g·L -1 whene 0.06 g·L -1 Arg added,in the fermentation with 2.5 L fermentor,when uracil 0.005 g·L -1 and Arg 0.06 g·L -1 were added,the rate of cell growth increased,maximum of specific growth rate,concentration of HA and HA molecular weight reached 0.67 h -1 ,5.2 g·L -1 and 2.15×10 6 Da from 0.54 h -1 ,4.2 g·L -1 ,2.0×10 6 Da,respectively.展开更多
文摘Based on the analysis of metabolic pathway Streptococcous zooepidemicus for hyaluronic acid (HA) synthesis,nucleotide,especially uracil,was considered to be important to cell growth and metabolism.When 0.005 g·L -1 uracil added in the media in which yeast extract as complex nitrogen source,cell growth and HA production were increased by 32% and 34% respectively.From analysis of amino acid in fermentation process,it was show that arginine(Arg)was needed for cell metabolism,and concentration of free Arg maintained at 0 g·L -1 in fermentation process,which was proposed to limit cell growth and HA production.By shake flask experiment HA concentration reached 0.510 g·L -1 whene 0.06 g·L -1 Arg added,in the fermentation with 2.5 L fermentor,when uracil 0.005 g·L -1 and Arg 0.06 g·L -1 were added,the rate of cell growth increased,maximum of specific growth rate,concentration of HA and HA molecular weight reached 0.67 h -1 ,5.2 g·L -1 and 2.15×10 6 Da from 0.54 h -1 ,4.2 g·L -1 ,2.0×10 6 Da,respectively.
