宫颈上皮内瘤变的阴道镜图像特征与意义

目的：探讨宫颈上皮内瘤变（CIN）的阴道镜图像特征与意义，评价阴道镜图像特征对CIN的诊断价值。方法：对2004年3月至2006年7月进行阴道镜检查的患者629例，行镜下定位取活组织送病理检查。结果：CIN组醋酸白色上皮、点状血管、镶嵌、腺口白环发生率均显著高于非CIN组，高级别CIN组镶嵌、点状血管的发生率显著高于低级别组。根据阴道镜拟诊和组织病理学结果进行对照。醋酸白色上皮或白环加点状血管或镶嵌对CIN诊断的符合率为84．8％。醋酸白色上皮或白环加点状血管加镶嵌三联征图像对CINⅢ诊断的敏感性为66．7％，特异性为94．6％，阴性预测值为99．1％；对CINⅠ～Ⅲ诊断的特异性为98．9％，阳性预测值为89．4％，阴性预测值为74．1％。结论：阴道镜是辅助诊断CIN的有效方法，醋酸白色上皮和白环对CIN的诊断有重要价值，如与点状血管和（或）镶嵌同时存在则特异性增高。初次阴道镜检可疑时应进行常期活检。

Salvianolic acid B alleviates oxidative stress in non-alcoholic fatty liver disease by mediating the SIRT3/FOXO1 signaling pathway

Salvianolic acid B(Sal B)is a polyphenolic antioxidant that has been shown to have anti-lipid accumulation,anti-inflammatory,and free oxygen radical scavenging activities in various diseases.Here,we aimed to examine whether Sal B could alleviate non-alcoholic fatty liver disease(NAFLD)and explore the possible mechanisms.Signaling pathways involved in oxidative stress,including SIRT3,SOD2,and FOXO1 pathways,were investigated by Western blotting analysis,RT-qPCR,and immunoprecipitation(IP).In the present study,oleic acid(OA)successfully induced lipid and peroxide accumulation,decreased SIRT3 expression,and increased FOXO1 acetylation.However,Sal B significantly reversed these trends.SIRT3 plasmid transfection further reduced the expression of acetylated FOXO1 and considerably enhanced the regulation of SIRT3 and acetylated FOXO1 induced by Sal B.Following SIRT3 siRNA transfection,Sal B-induced down-regulation of acetylated FOXO1 was blocked,suggesting that Sal B-mediated protection occurred through SIRT3-mediated FOXO1 deacetylation.The SIRT3/FOXO1 pathway was a critical therapeutic target for controlling oxidative stress in NAFLD,and Sal B conferred protection against OA-induced hepatic steatosis and oxidative stress through SIRT3-mediated FOXO1 deacetylation.