In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T.mucronatum was identified by SRAP(Sequence-related Amplified Polymorphism) markers.Firstly,48 SRAP primer combinations were random...In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T.mucronatum was identified by SRAP(Sequence-related Amplified Polymorphism) markers.Firstly,48 SRAP primer combinations were randomly selected and polymorphism primers between two parents(T.distichum and T.mucronatum) were selected.As a result,all primers amplifid clear bands,and 29 primers had polymorphism bands of two parents,accounted for 60.4% of all primers,and 14 primer combinations with specific bands both two samples.Authenticity of 4 progenies from T.distichum and T.mucronatum was identified by 12 polymorphism primer combinations selected from above 29 polymorphism primer combinations.The results showed that 4 progenies were all identified to be true hybrids owing to their specific bands from male parent.Among the primer combinations,Me9+Em10 produced specific bands of both male parent and female parent in every progeny,that is to say,with this one primer combination alone,the status of 4 progenies can be identified.Efficiency of molecule identification were much improved.The results of hybrids identification provided a solid evidence for the hybrids further study and application.The experimental results also proved that SRAP molecular marker technology can be effectively applied in the researches on Taxodium.展开更多
The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.div...The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.divaricata leaf,with relative molecular weight of 335 and 365,respectively and lower content.The constituent with relative molecular weight 335 is a retronecine-type alkaloid,and identified as integerrimine;another constituent with relative molecular weight 365 is an otonecine-type alkaloid,speculated to be isomer of usaramine or 18-hydroxyinterrimine.展开更多
The content of polyphenolic compounds including chlorogenic acid,vitexin-4″-O-glucoside,vitexin rhamnoside,rutin,vitexin,hyperoside in leaf of Crataegus pinnatifida Bunge var.major N.E.Br.at different times was deter...The content of polyphenolic compounds including chlorogenic acid,vitexin-4″-O-glucoside,vitexin rhamnoside,rutin,vitexin,hyperoside in leaf of Crataegus pinnatifida Bunge var.major N.E.Br.at different times was determined by HPLC.The results show that the content of polyphenolic compounds in different times is various.In April,the total content of polyphenolic compounds and the contents of chlorogenic acid,vitexin-4″-O-glucoside,vitexin rhamnoside,rutin reach the peak.And the content of every compound and the total content of these compounds decrease gradually with the plant growing,and reach the lowest point in July to August.Therefore,in order to extract polyphenolic compounds,the optimal harvest time for C.pinnatifida var.major leaf is April.展开更多
文摘In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T.mucronatum was identified by SRAP(Sequence-related Amplified Polymorphism) markers.Firstly,48 SRAP primer combinations were randomly selected and polymorphism primers between two parents(T.distichum and T.mucronatum) were selected.As a result,all primers amplifid clear bands,and 29 primers had polymorphism bands of two parents,accounted for 60.4% of all primers,and 14 primer combinations with specific bands both two samples.Authenticity of 4 progenies from T.distichum and T.mucronatum was identified by 12 polymorphism primer combinations selected from above 29 polymorphism primer combinations.The results showed that 4 progenies were all identified to be true hybrids owing to their specific bands from male parent.Among the primer combinations,Me9+Em10 produced specific bands of both male parent and female parent in every progeny,that is to say,with this one primer combination alone,the status of 4 progenies can be identified.Efficiency of molecule identification were much improved.The results of hybrids identification provided a solid evidence for the hybrids further study and application.The experimental results also proved that SRAP molecular marker technology can be effectively applied in the researches on Taxodium.
文摘The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.divaricata leaf,with relative molecular weight of 335 and 365,respectively and lower content.The constituent with relative molecular weight 335 is a retronecine-type alkaloid,and identified as integerrimine;another constituent with relative molecular weight 365 is an otonecine-type alkaloid,speculated to be isomer of usaramine or 18-hydroxyinterrimine.
文摘The content of polyphenolic compounds including chlorogenic acid,vitexin-4″-O-glucoside,vitexin rhamnoside,rutin,vitexin,hyperoside in leaf of Crataegus pinnatifida Bunge var.major N.E.Br.at different times was determined by HPLC.The results show that the content of polyphenolic compounds in different times is various.In April,the total content of polyphenolic compounds and the contents of chlorogenic acid,vitexin-4″-O-glucoside,vitexin rhamnoside,rutin reach the peak.And the content of every compound and the total content of these compounds decrease gradually with the plant growing,and reach the lowest point in July to August.Therefore,in order to extract polyphenolic compounds,the optimal harvest time for C.pinnatifida var.major leaf is April.
