建立了烯肟菌胺原药的高效液相色谱分析方法。采用Phenomenex Luna C18(2)柱,紫外可变波长检测器,检测波长275nm;以乙腈-水(体积比85∶15)为流动相,流速0.8mL/min。外标法对主要成分进行定量分析,在0.05～10.0mg/L范围内线性相关系数为0...建立了烯肟菌胺原药的高效液相色谱分析方法。采用Phenomenex Luna C18(2)柱,紫外可变波长检测器,检测波长275nm;以乙腈-水(体积比85∶15)为流动相,流速0.8mL/min。外标法对主要成分进行定量分析,在0.05～10.0mg/L范围内线性相关系数为0.9999,平均回收率为96.2%,样品测定的标准偏差0.0285,变异系数为0.2953%。展开更多
[目的]通过2年3地的田间试验及残留检测,明确唑胺菌酯在黄瓜及土壤中的消解动态和最终残留量。[方法]消解动态试验按剂量300 g a.i./hm2施药1次;最终残留试验按300 g a.i./hm2(高剂量)和200 g a.i./hm2(低剂量)分别施药4、5次;采用高效...[目的]通过2年3地的田间试验及残留检测,明确唑胺菌酯在黄瓜及土壤中的消解动态和最终残留量。[方法]消解动态试验按剂量300 g a.i./hm2施药1次;最终残留试验按300 g a.i./hm2(高剂量)和200 g a.i./hm2(低剂量)分别施药4、5次;采用高效液相色谱法对20%唑胺菌酯悬浮剂有效成分进行检测。[结果]2年3地的消解动态试验结果表明:唑胺菌酯在黄瓜和土壤中的消解半衰期分别为2.86~5.63、4.61~13.25 d。最终残留试验结果表明:唑胺菌酯在黄瓜和土壤中的最终残留量分别为<0.020~0.380、<0.020~1.134 mg/kg。[结论]建议唑胺菌酯在黄瓜中的最大残留限量为0.08 mg/kg;20%唑胺菌酯悬浮剂按其推荐剂量200 g a.i./hm2在黄瓜上施用4次,安全间隔期为3 d。展开更多
A series of novel 2,6-dichloro-3,5-dinitrotoluene derivatives were designed, synthesized in the present study, and their antitumor activities against five cell lines（A431, HepG2, A549, HT-29 and HEK-293） were tested...A series of novel 2,6-dichloro-3,5-dinitrotoluene derivatives were designed, synthesized in the present study, and their antitumor activities against five cell lines（A431, HepG2, A549, HT-29 and HEK-293） were tested. Most of the compounds exhibited moderate-to-significant cytotoxicity and high selectivity against one or more cell lines in comparison with cisplatin. Studies on their preliminary structure-activity relationships（SARs） indicated that compounds containing phenyl（piperazin-1-yl） methanone groups, especially chlorine atom at 4-position of the phenyl ring, were more effective. Compound 4g was found to be the most potent derivative with IC_（50） values of 1.04, 3.20, 6.93, 4.10 and 20.15 μmol/L against A431, HepG2, A549, HT-29 and HEK-293 cell lines, respectively, which was better than positive control cisplatin, one of the most clinically used chemotherapeutic drugs.展开更多
文摘建立了烯肟菌胺原药的高效液相色谱分析方法。采用Phenomenex Luna C18(2)柱,紫外可变波长检测器,检测波长275nm;以乙腈-水(体积比85∶15)为流动相,流速0.8mL/min。外标法对主要成分进行定量分析,在0.05～10.0mg/L范围内线性相关系数为0.9999,平均回收率为96.2%,样品测定的标准偏差0.0285,变异系数为0.2953%。
文摘[目的]通过2年3地的田间试验及残留检测,明确唑胺菌酯在黄瓜及土壤中的消解动态和最终残留量。[方法]消解动态试验按剂量300 g a.i./hm2施药1次;最终残留试验按300 g a.i./hm2(高剂量)和200 g a.i./hm2(低剂量)分别施药4、5次;采用高效液相色谱法对20%唑胺菌酯悬浮剂有效成分进行检测。[结果]2年3地的消解动态试验结果表明:唑胺菌酯在黄瓜和土壤中的消解半衰期分别为2.86~5.63、4.61~13.25 d。最终残留试验结果表明:唑胺菌酯在黄瓜和土壤中的最终残留量分别为<0.020~0.380、<0.020~1.134 mg/kg。[结论]建议唑胺菌酯在黄瓜中的最大残留限量为0.08 mg/kg;20%唑胺菌酯悬浮剂按其推荐剂量200 g a.i./hm2在黄瓜上施用4次,安全间隔期为3 d。
基金The Natural Science Foundation of Liaoning province(Grant No.20170540730)
文摘A series of novel 2,6-dichloro-3,5-dinitrotoluene derivatives were designed, synthesized in the present study, and their antitumor activities against five cell lines（A431, HepG2, A549, HT-29 and HEK-293） were tested. Most of the compounds exhibited moderate-to-significant cytotoxicity and high selectivity against one or more cell lines in comparison with cisplatin. Studies on their preliminary structure-activity relationships（SARs） indicated that compounds containing phenyl（piperazin-1-yl） methanone groups, especially chlorine atom at 4-position of the phenyl ring, were more effective. Compound 4g was found to be the most potent derivative with IC_（50） values of 1.04, 3.20, 6.93, 4.10 and 20.15 μmol/L against A431, HepG2, A549, HT-29 and HEK-293 cell lines, respectively, which was better than positive control cisplatin, one of the most clinically used chemotherapeutic drugs.
