To analyze P16 gene mutation and its role in children’s acute leukemia. The deleted mutation was analyzed with the amplified exonl and exon2 of P16 gene by PCR. The amplified product of exon2 underwent CDGE (constant...To analyze P16 gene mutation and its role in children’s acute leukemia. The deleted mutation was analyzed with the amplified exonl and exon2 of P16 gene by PCR. The amplified product of exon2 underwent CDGE (constant denatured gel electrophoresis) as a point mutation analysis. Resulte showed that the rate of the deletion mutation was very high. There were 18 deletion mutational cases and 6 point mutations among 57 clinical specimens, the total mutation rate being 31.6%. The mutation rate of P16 gene was 64.7% in acute lymphocytic leukemia (ALL) and only 8.7% in acute myeloid leukemia (AML). Significant difference was found between them. Therefore the P16 gene mutation played an important role in the development of chileren’s acute leukomia. “GC” DNA repetitive sequence of P16 gene was responsible for higher deletion mutation than point mutation.展开更多
文摘To analyze P16 gene mutation and its role in children’s acute leukemia. The deleted mutation was analyzed with the amplified exonl and exon2 of P16 gene by PCR. The amplified product of exon2 underwent CDGE (constant denatured gel electrophoresis) as a point mutation analysis. Resulte showed that the rate of the deletion mutation was very high. There were 18 deletion mutational cases and 6 point mutations among 57 clinical specimens, the total mutation rate being 31.6%. The mutation rate of P16 gene was 64.7% in acute lymphocytic leukemia (ALL) and only 8.7% in acute myeloid leukemia (AML). Significant difference was found between them. Therefore the P16 gene mutation played an important role in the development of chileren’s acute leukomia. “GC” DNA repetitive sequence of P16 gene was responsible for higher deletion mutation than point mutation.
