[Objective] This study aimed to explore the pathogenic mechanism of E., cherichia coll. [Method] An E. coil strain isolated from raccoon dogs in vivo was studied, which had been identified, PCR was used to detect the ...[Objective] This study aimed to explore the pathogenic mechanism of E., cherichia coll. [Method] An E. coil strain isolated from raccoon dogs in vivo was studied, which had been identified, PCR was used to detect the gene of irp2 （301 bp） and fyuA （953 bp） related to E. coil virulence and PCR products were s, quenced. [Result] The genes of irp2 and fyuA were successfully amplified in boi strains isolated from raccoon dogs. Compared with the GenBank, the identity of tTr irp2 gene sequence and the fyuA gene sequence of the strain reached 98.5% 99.2% and 98.9%-100% respectively. Compared with each other, the identity of tt- two gene sequences of irp2 was 99.3%, and that between the two fyuA gene se quences was 98.9%. [Conclusion] This study provided scientific experimental data fi E. coil pathogenicity, prevention, diagnosis and treatment.展开更多
[Objective] This study aimed to investigate effects of Echinacea compound on the immune function of weaned piglets. [Method] Eighty Duroc x Landrace x Yorkshire crossbred piglets were randomly divided into four groups...[Objective] This study aimed to investigate effects of Echinacea compound on the immune function of weaned piglets. [Method] Eighty Duroc x Landrace x Yorkshire crossbred piglets were randomly divided into four groups： control group （drug-free group）, 1.5% close group, 1.0% dose group and 0.5% dose group. Blood samples of piglets were collected at 20, 35, 50, 60, 70 and 80 days old, respective- ly, to determine neutrophil leukocyte percentage in peripheral blood, lymphocyte trapsformation rate and the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder. [Result] Applying a certain dose of Echinacea compound could significantly increase neutrophil leukocyte per- centage in peripheral blood and lymphocyte transformation rate （P〈0.05）, and ex- tremely significantly improve the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder （P〈0.01）. [Conclusion] Echi- nacea compound has played a certain role in promoting nonspecific and specific im- mune function of piglets.展开更多
Objective] The passing rates of porcine reproductive and respiratory syn-drome virus （PRRSV） vaccine and classical swine fever virus （CSFV） vaccine were investigated in this paper. The PRRSV vaccine was injected i...Objective] The passing rates of porcine reproductive and respiratory syn-drome virus （PRRSV） vaccine and classical swine fever virus （CSFV） vaccine were investigated in this paper. The PRRSV vaccine was injected in 14-d-old piglets and the CSFV vaccine was injected in 25-d-old piglets. [Method] The antibody expres-sion amounts were detected with the PRRSV antibody ELlSA detection kit and the CSFV antibody ELlSA detection kit. [Result] The antibody positive rates were 6.7%, 26.7%, 85% and 100% 3, 7, 14 and 30 d after the vaccination of PRRSV vaccine. While for the CSFV vaccine, 13%, 40%, 58.3% and 83.3% of the immune individu-als were antibody positive 3, 7, 14 and 30 d after the vaccination. [Conclusion] The PRRSV vaccine showed the lowest antibody tilter 3 d after the inoculation, but had a great vaccination effect 30 d after the inoculation. The piglets were not immune enough against viral invasion 3 d after the inoculation of CSFV vaccine. Although the antibody positive rate of PRRSV and CSFV vaccines al reached the required level by the Ministry of Agriculture （70%） 30 d after the inoculation, the vaccination of CSFV vaccine could not achieve the ideal effect of 100%.展开更多
基金Supported by China Postdoctoral Science Fond(20100470565)Science and Technology Support Program of Hebei Province(10960408D)Science and Technology Development Project of Qinhuangdao City(201101A182)~~
文摘[Objective] This study aimed to explore the pathogenic mechanism of E., cherichia coll. [Method] An E. coil strain isolated from raccoon dogs in vivo was studied, which had been identified, PCR was used to detect the gene of irp2 （301 bp） and fyuA （953 bp） related to E. coil virulence and PCR products were s, quenced. [Result] The genes of irp2 and fyuA were successfully amplified in boi strains isolated from raccoon dogs. Compared with the GenBank, the identity of tTr irp2 gene sequence and the fyuA gene sequence of the strain reached 98.5% 99.2% and 98.9%-100% respectively. Compared with each other, the identity of tt- two gene sequences of irp2 was 99.3%, and that between the two fyuA gene se quences was 98.9%. [Conclusion] This study provided scientific experimental data fi E. coil pathogenicity, prevention, diagnosis and treatment.
基金Supported by National Nature Science Foundation of China(31472230)Nature Science Foundation of Hebei Province(C2014407068)+2 种基金Project of Science and Technology Department of Hebei Province(14966610D)Project of Shijiazhuang Municipal Science and Technology Bureau(131200063A)Support Program of 100 Outstanding Innovative Talents of Hebei Education Department(ZH2011244)~~
文摘[Objective] This study aimed to investigate effects of Echinacea compound on the immune function of weaned piglets. [Method] Eighty Duroc x Landrace x Yorkshire crossbred piglets were randomly divided into four groups： control group （drug-free group）, 1.5% close group, 1.0% dose group and 0.5% dose group. Blood samples of piglets were collected at 20, 35, 50, 60, 70 and 80 days old, respective- ly, to determine neutrophil leukocyte percentage in peripheral blood, lymphocyte trapsformation rate and the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder. [Result] Applying a certain dose of Echinacea compound could significantly increase neutrophil leukocyte per- centage in peripheral blood and lymphocyte transformation rate （P〈0.05）, and ex- tremely significantly improve the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder （P〈0.01）. [Conclusion] Echi- nacea compound has played a certain role in promoting nonspecific and specific im- mune function of piglets.
基金Supported by Agricultural Science and Technology Achievement Transformation Fund of Ministry of Science and Technology(2012GB2A200045)Fund Project of Hebei Province Department of Education(2008449)Special Project of Qinhuangdao Municipal Bureau of Science and Technology(201101A181)~~
文摘Objective] The passing rates of porcine reproductive and respiratory syn-drome virus （PRRSV） vaccine and classical swine fever virus （CSFV） vaccine were investigated in this paper. The PRRSV vaccine was injected in 14-d-old piglets and the CSFV vaccine was injected in 25-d-old piglets. [Method] The antibody expres-sion amounts were detected with the PRRSV antibody ELlSA detection kit and the CSFV antibody ELlSA detection kit. [Result] The antibody positive rates were 6.7%, 26.7%, 85% and 100% 3, 7, 14 and 30 d after the vaccination of PRRSV vaccine. While for the CSFV vaccine, 13%, 40%, 58.3% and 83.3% of the immune individu-als were antibody positive 3, 7, 14 and 30 d after the vaccination. [Conclusion] The PRRSV vaccine showed the lowest antibody tilter 3 d after the inoculation, but had a great vaccination effect 30 d after the inoculation. The piglets were not immune enough against viral invasion 3 d after the inoculation of CSFV vaccine. Although the antibody positive rate of PRRSV and CSFV vaccines al reached the required level by the Ministry of Agriculture （70%） 30 d after the inoculation, the vaccination of CSFV vaccine could not achieve the ideal effect of 100%.
