The Chinese Herbal Formula Weichang’an Reduces the Proliferation of Human Gastric Cancer Cells via Mitochondrial Apoptosis

Objective The aim of the study was to investigate the effects of Chinese herbal formula Weichang’an(WCA)on the proliferation and mitochondria-mediated apoptosis of human gastric cancer cells.Methods Cell Counting Kit-8(CCK8)was used to evaluate the antiproliferative activity ofWCA on MKN45 cells;Giemsa staining was used to investigate cell colony formation;flow cytometry was used to analyze cell cycle,apoptosis rate,and caspases activation;and Hoechst staining was used to analyze the morphology of cell nuclei.The mitochondrial membrane potential was analyzed by both flow cytometric measurements and fluorescence microscopy.Western blot was used to analyze the protein levels of pro-caspase-3,Bcl-2,Bax,and Bcl-X.MKN45 cells were subcutaneously injected into the right forelimb of 16 nude mice to establish the tumor xenograft model,and a total of 12 nude mouse tumor xenograft models were successfully created.The nude mice were divided into the control group and the WCA-treated group.The two groups received normal saline and WCA treatment at 35.49 g/kg by a single daily oral gavage for 21 days.The body weight and tumor size of the nude mice were measured twice a week.The ultrastructural changes of subcutaneous tumors were observed by a transmission electron microscope.Results WCA can suppress cell proliferation and colony formation.It can also induce changes in the mitochondrial membrane potential and increase the activities of caspases-3,caspases-8,and caspases-9 in MKN45 cells.WCA induced S-phase arrest and apoptosis in MKN45 cells,and decreased the expression levels of antiapoptotic proteins Bcl-2 and Bcl-X in MKN45 cells,while increasing the expression levels of the proapoptotic proteins Bax and pro-caspase-3 compared with the control group.WCA inhibited the growth of a xenografted MKN45 tumor in nude mice,and the protein levels of Bax and pro-caspase-3 were significantly increased,while Bcl-X and Bcl-2 were reduced compared with the control group.The differences are statistically significant(p<0.05).Conclusion WCA could suppress the proliferation of gastric cancer cells via mitochondrial apoptosis.

人工肝支持系统治疗肝衰竭患者并发下肢深静脉血栓的危险因素

目的调查分析人工肝支持系统治疗高龄肝衰竭患者并发下肢深静脉血栓(DVT)风险因素。方法选取医院2017年6月—2020年10月收纳的行人工肝支持系统治疗的肝衰竭患者118例为研究对象,将并发DVT的36例患者作为病例组,非DVT患者72例作为对照组,收集患者临床基础资料并分析DVT风险因素。结果单因素及多因素Logistic分析显示,年龄、血清D-二聚体浓度、股静脉置管、急性出血性坏死胰腺炎等为DVT的风险因素(P<0.05)。结论行人工肝支持系统治疗的肝衰竭患者出现DVT与年龄、血清D-二聚体浓度、股静脉置管、急性出血性坏死胰腺炎等相关,需加强风险因素防范,采取合理措施针对性干预。

The Mechanism pf Weichang'an in Inducing Apoptosis of Gastric Cancer SGC7901 Cells

Objective:To investigate the effect of Chinese medicine Compound Weichang'an(胃肠安)for invig-orating the spleen on apoptosis of gastric cancer SGC7901 cells and its possible mechanism.Methods:The gas-trie cancer SGC-7901 cells were divided into different mass concentration groups(0 mg·L^(-1),500 mg·L^(-1)1000 mg·L^(-1),1500 mg·L^(-1),2000 mg·L^(-1)).CCK8 and monoclonal test were applied to detect prolifera-tion ability;comet assay was used to detect DNA damage.After DCFH-DA fluorescent labeling,the level of ROS activity was detected by flow cytometer;after AnnexinV-FTC/PI double labeling,the proportion of apoptotic ellls was detected by flow cytometer;after JC-1 staining,the mi tochondri almembrane potential was detected by flow cytometer;after FTTC-DEVD-FMK staining,the ratio of Caspase activity was detected by flow cytometer.Results:Weichang an inhibited cell proliferation and reduced cell colony formation in a time-dose-dependent manner;the results of comet electrophoresis showed that Weichang'an could induce DNA damage in gastric cancer cells;com-pared with control group.the ratio of Weichang'an's intervention with the apoptosis of gastric cancer cells in-creased(P<0.05),the mitochondrial membrane potential decreased(P<0.05),the activity of Caspase3 and Caspase9 increased(P<0.05),and the intracellular ROS level increased(P<0.05).Among them,the effect of Weichang'an treatment group(1000 mg·L^(-1))was the most significant.Conclusion:Weichang'an has an inhibi-tory effect on the proliferation of gastric cancer SGC7901 cells and can induce cell apoptosis.Its mechanism may be related with the ROS-mediated pathway of mitochondrial apoptosis and DNA damage.

脑出血的临床与CT动态观察48例报告

脑出血的临床与ＣＴ动态观察４８例报告赵建华，李哲诚，李德霞脑出血的临床和ＣＴ报告较多，而脑出血的动状观察与ＣＴ扫描演变过程之间的关系国内报告较少，现将我科近年观察的４８例总结分析如下：１资料和方法１．１１临床资料：共４８例，男３８例，女１０例，年龄１...

郑州地区围生期孕妇母婴健康素养现状调查

目的调查郑州地区围生期孕妇母婴健康素养水平情况,并分析其影响因素,为更好地开展母婴健康教育提供科学依据。方法以2023年5月在郑州市3家医院建档、定期产检以及计划住院分娩的围生期孕妇为研究对象,参考利用《母婴健康素养促进》项目设计的《孕妇母婴健康素养调查问卷》对纳入对象进行健康素养的问卷调查,并采用多重线性回归分析影响健康素养的相关因素。结果共纳入1503名围生期孕妇,年龄为20~49岁,平均(32.36±5.72)岁。母婴健康素养(35题)的平均得分为(56.77±11.51)分,具备母婴健康素养的孕妇46人,具备率为3.06%,各维度中具备母婴基本知识人数最高为243人,具备率16.17%;其次为婴幼儿知识(13.24%)、基本技能(10.58%)和生活方式(9.65%)。偏向生活常识类母婴健康知识的知晓率较高,偏向具备生理指标类的内容知晓率较差。多重线性回归分析结果显示文化程度(β′=0.225、1.211、0.449)、医疗行业(β′=0.384)、经产妇(β′=0.690)和参加过孕妇学校课程(β′=1.291)是影响围生期孕妇母婴健康素养的独立影响因素。结论郑州地区围生期孕妇母婴健康素养水平整体较低,应针对低文化程度的初产妇这一重点围生期孕妇人群加强关于母婴健康生理性指标实施全面健康素养教育,并加强多方式健康知识宣传教育工作,以满足围生期孕妇母婴健康知识掌握的需求。

Influence of scarring moxibustion on serum melatonin and neuropeptide Y expressions in the insomnia model rats

Objective:To explore the influence of scarring moxibustion on the changes of serum melatonin(MT)and neuropeptide Y(NPY)expressions in the insomnia model rats.Methods:A total of 40 SD rats were randomly divided into a blank group,a model group,a scarring moxibustion group,an embedding needling group and a mild moxibustion group,8 rats in each group.The intraperitoneal injection of p-chlorophenylalanine(PCPA)was adopted to establish the insomnia model rats and the corresponding interventions were given,consecutively for 6 days.Separately,before modeling and the first intervention as well as before sample collection,the body weight was measured and the serum was collected in the insomnia model rats.The enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum MT and NPY.Results:Compared with the blank group,the body weight of rat in the model group was lower(P<0.01)and the levels of MT and NPY were lower in the model group(both P<0.05).Compared with the model group,in the scarring moxibustion group,the embedding needling group and the mild moxibustion group,the body weight and the levels of MT and NPY of the insomnia model rats were all higher(all P<0.05).Compared with the embedding needling group and the mild moxibustion group,the body weight and the levels of MT and NPY the insomnia model rats were all higher in the scarring moxibustion group(all P<0.05).In comparison between the embedding needling group and the mild moxibustion group,there were no statistical differences in the body weight and the levels of MT and NPY(all P>0.05).Conclusion:The regulation mechanism of sleep-wake circadian rhythm may be related to the levels of serum MT and NPY in the insomnia model rats.Scarring moxibustion increases the levels of MT and NPY more effectively and displays the effect in the intervention as compared with embedding needling and mild moxibustion in the insomnia model rats.

Research progress of sacubitril/valsartan in heart failure patients with preserved ejection fraction

With the launch of sacubitril/valsartan(ARNI),there are new options for the treatment of heart failure(HF).However,ARNI is currently only used in HF patients with reduced ejection fraction(HFrEF).No evidence shows that no modern treatment can reduce mortality in HF patients with preserved ejection fraction(HFpEF).Therefore,it is urgently necessary clarify whether ARNI can be used in the treatment of HFpEF.In the present study,we summarized the research progress of ARNI in the treatment of HFpEF.

Pharmacological and clinical evaluation of a new anti-flu drug, baloxavir marboxil

Baloxavir marboxil is a polymerase acidic(PA)endonuclease inhibitor,which is approved by the U.S Food and Drug Administration(FDA)on October 25,2018,for the treatment of uncomplicated influenza patients aged 12 years and older.In the present work,we reviewed the pharmacodynamics,pharmacokinetics,drug interactions,clinical trials and adverse reactions of baloxavir marboxil.