Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collect...Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.展开更多
Objectives:To observe the clinical effect on the sequela of peripheral facial paralysis treated with the penetrating-sticking-pulling technique of acupuncture.Methods:A total of 53 patients with the sequela of periphe...Objectives:To observe the clinical effect on the sequela of peripheral facial paralysis treated with the penetrating-sticking-pulling technique of acupuncture.Methods:A total of 53 patients with the sequela of peripheral facial paralysis were diagnosed and divided into an observation group(26 cases)and a control group(27 cases)according to the visiting sequence.In the observation group,the penetrating-sticking-pulling technique of acupuncture was adopted.The Sibai(四白ST 2),Quanliao(颧髎 SI 18)and Jiache(颊车ST 6)et al.were needling toward Dicang(地仓ST 4)separately.The combined acupoints were selected according to clinical symptoms of individual patient.In the control group,the routine acupuncture was used,in which,ST 2,EX-HN 5,ST 7,ST 6,and ST 4 as well as Hegu(合谷LI 4)on the healthy side were selected.The treatment was given once every two days and a 7-treatment made one course.There were 3 days at interval between the courses.After 2 courses of treatment,the facial nerve function,e.g.,Hourse-Brackmann(H-B)grading,Sunnybrook score and clinical effect were observed.Results:①After treatment,H-B grading and Sunnybrook score were better than those before treatment in either of the observation group or the control group(P<0.05,P<0.01).H-B grading and Sunnybrook score were(2.23±0.64)and(96.62±2.59),respectively,in the observation group after treatment and they were(3.21±0.43)and(80.33±3.16)respectively in the control group.H-B grading and Sunnybrook score in the observation group were better than the control group,indicating the significant differences(P<0.05,P<0.01).②The curative rate and the total effective rate were 69.23%and 96.15%,respectively,in the observation group and they were 37.04%and 81.48%,respectively,in the control group,presenting the significant differences(both P<0.05).Conclusion:The penetrating-sticking-pulling technique of acupuncture achieves the definite effect on the sequela of peripheral facial paralysis and this therapy deserves to be promoted in clinical practice.展开更多
文摘Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.
文摘Objectives:To observe the clinical effect on the sequela of peripheral facial paralysis treated with the penetrating-sticking-pulling technique of acupuncture.Methods:A total of 53 patients with the sequela of peripheral facial paralysis were diagnosed and divided into an observation group(26 cases)and a control group(27 cases)according to the visiting sequence.In the observation group,the penetrating-sticking-pulling technique of acupuncture was adopted.The Sibai(四白ST 2),Quanliao(颧髎 SI 18)and Jiache(颊车ST 6)et al.were needling toward Dicang(地仓ST 4)separately.The combined acupoints were selected according to clinical symptoms of individual patient.In the control group,the routine acupuncture was used,in which,ST 2,EX-HN 5,ST 7,ST 6,and ST 4 as well as Hegu(合谷LI 4)on the healthy side were selected.The treatment was given once every two days and a 7-treatment made one course.There were 3 days at interval between the courses.After 2 courses of treatment,the facial nerve function,e.g.,Hourse-Brackmann(H-B)grading,Sunnybrook score and clinical effect were observed.Results:①After treatment,H-B grading and Sunnybrook score were better than those before treatment in either of the observation group or the control group(P<0.05,P<0.01).H-B grading and Sunnybrook score were(2.23±0.64)and(96.62±2.59),respectively,in the observation group after treatment and they were(3.21±0.43)and(80.33±3.16)respectively in the control group.H-B grading and Sunnybrook score in the observation group were better than the control group,indicating the significant differences(P<0.05,P<0.01).②The curative rate and the total effective rate were 69.23%and 96.15%,respectively,in the observation group and they were 37.04%and 81.48%,respectively,in the control group,presenting the significant differences(both P<0.05).Conclusion:The penetrating-sticking-pulling technique of acupuncture achieves the definite effect on the sequela of peripheral facial paralysis and this therapy deserves to be promoted in clinical practice.
