The Role of Predominant Expression of Th2 Type Cytokines Gene in the Genesis and Development of Human Gliomas

Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as Th2 type cytokines, the biological activity of cytokines in the supernatant of glioma cell lines was assayed by ELISA method, and the gene expression of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR.Results: There was predominant expression of Th2 type cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines, but there was no such expression in normal brain tissues.Conclusion: It suggested that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosupressive status of human glioma patients. The predominant expression of Th2 type cytokines may play an important role in the genesis and development of human gliomas. Key words glioma - Th1/Th2 - gene expression - RT-PCR This project was supported by a grant from National Natural Sciences foundation of China (No. 30271335).

颅脑外伤后患者的焦虑症状及相关因素研究

目的 研究颅脑外伤后患者的焦虑症状及相关因素。方法 对63例急性期颅脑外伤患者采用标准化测评工具,包括《格拉斯哥昏迷评分(GCS)》、《汉密顿焦虑量表(HAMA)》、《社会支持评定量表》、《日常生活能力量表》及自制颅脑外伤患者人口学与相关因素调查表等,逐例进行现场临床测评,汇总资料进行整理、分析。所有统计分析均使用SAS软件包完成。结果 颅脑外伤患者焦虑症状的发生率为57.14％,其中重度、中度、轻度焦虑症状的发生率分别为9.52％、7.93％、39.68％。主要影响因素有性格、社会支持、病理征、外伤部位、经济状况及外伤原因。结论 提示器质性因素在焦虑的产生中不起决定性作用,而个体的生理心理特点和社会环境因素在焦虑产生中具有重要意义。