目的 探讨三磷酸腺苷结合转运蛋白G超家族成员2（adenosine triphosphate-binding cassette superfamily G member 2,ABCG2）的m RNA和蛋白表达水平与乳腺癌患者化疗疗效和耐药性的关系。方法 选取确诊为乳腺癌的患者24例作为研究对象,...目的 探讨三磷酸腺苷结合转运蛋白G超家族成员2（adenosine triphosphate-binding cassette superfamily G member 2,ABCG2）的m RNA和蛋白表达水平与乳腺癌患者化疗疗效和耐药性的关系。方法 选取确诊为乳腺癌的患者24例作为研究对象,所有患者均经手术治疗后辅以蒽环类药物化疗,根据WHO疗效评价标准评估患者化疗后效果和耐药性,根据化疗效果将患者分为有效组、稳定组及进展组;分别于化疗前后利用q PCR和ELISA法检测患者乳腺组织中ABCG2的m RNA和蛋白表达水平。结果 化疗后总有效率为41.67%,即仍有58.33%的患者出现耐药性;化疗后患者ABCG2 m RNA和蛋白表达水平均显著高于化疗前（P〈0.05）;化疗有效组的患者ABCG2 m RNA和蛋白表达水平显著低于化疗稳定组及进展组的患者（P〈0.05）;化疗稳定组患者ABCG2 m RNA和蛋白表达水平显著低于进展组（P〈0.05）。结论ABCG2的表达水平与乳腺癌患者化疗疗效和耐药性相关,ABCG2表达水平越高,促进药物排泄的能力越强,肿瘤细胞的耐药性越显著,化疗疗效越差。展开更多
目的 分析15例嗜麦芽窄食单胞菌败血症患者的临床因素及菌株同源性.方法 收集2006年9至12月浙江大学医学院附属第一医院肝移植病房的15例嗜麦芽窄食单胞菌败血症患者的临床资料,采用浓度梯度法(etest)测定15株细菌对10种常用抗菌药物的...目的 分析15例嗜麦芽窄食单胞菌败血症患者的临床因素及菌株同源性.方法 收集2006年9至12月浙江大学医学院附属第一医院肝移植病房的15例嗜麦芽窄食单胞菌败血症患者的临床资料,采用浓度梯度法(etest)测定15株细菌对10种常用抗菌药物的最小抑菌浓度(mic),通过whonet5软件对菌株进行抗生素分型,脉冲场凝胶电泳(pfge)分析菌株同源性.结果 所有患者均有机械通气史和长期使用广谱β-内酰胺类和喹诺酮类药物史,15株嗜麦芽窄食单胞菌在体外对米诺环素、复方磺胺甲恶唑和环丙沙星敏感性最好.抗生素型和pfge分析提示嗜麦芽窄食单胞菌败血症为局部流行,在不同时间段有克隆a(9株)和克隆b(6株)两个克隆株流行.结论 机械通气可能与肝移植患者发生嗜麦芽窄食单胞菌败血症有关.
abstract：
objective to investigate the related clinical factors and homology of strains in stenotrophomonas maltophilia (s. maltophilia) infections in 15 patients with liver transplantation. methods fifteen patients with s. maltophilia infection from september to december 2006 were enrolled and their clinical data were collected. minimal inhibitory concentrations (mics) of 10 antimierobial agents against s. maltophilia were determined by etest strips. antibiogram was carried out by resistance analysis assembly with whonet 5 software. the genomic dna of all the isolates was digested with xbal and subjected to pulse-field gel electrophoresis (pfge). results all patients received mechanical ventilation during the treatment and had a history of long-term use of extended-spectrum β-lactamases and quinolones. mics of 10 antimicrobial agents indicated that s. maltophilia were susceptible to several antimicrobial agents including compound sulfamethoxazole and ciprofloxacin, but the best active agent against these resistant isolates was minocycline in vitro. the results of all 15 s. maltophilia antibiograms were accordance with pfge patterns. all 15 s. maltophilia isolates were classified as 2 pfge patterns: 9 for pattern a and 6 for pattern b. conclusion mechanical ventilation might be associated with the s. maltophilia septicemia in patients with liver transplantation.展开更多
文摘目的 探讨三磷酸腺苷结合转运蛋白G超家族成员2（adenosine triphosphate-binding cassette superfamily G member 2,ABCG2）的m RNA和蛋白表达水平与乳腺癌患者化疗疗效和耐药性的关系。方法 选取确诊为乳腺癌的患者24例作为研究对象,所有患者均经手术治疗后辅以蒽环类药物化疗,根据WHO疗效评价标准评估患者化疗后效果和耐药性,根据化疗效果将患者分为有效组、稳定组及进展组;分别于化疗前后利用q PCR和ELISA法检测患者乳腺组织中ABCG2的m RNA和蛋白表达水平。结果 化疗后总有效率为41.67%,即仍有58.33%的患者出现耐药性;化疗后患者ABCG2 m RNA和蛋白表达水平均显著高于化疗前（P〈0.05）;化疗有效组的患者ABCG2 m RNA和蛋白表达水平显著低于化疗稳定组及进展组的患者（P〈0.05）;化疗稳定组患者ABCG2 m RNA和蛋白表达水平显著低于进展组（P〈0.05）。结论ABCG2的表达水平与乳腺癌患者化疗疗效和耐药性相关,ABCG2表达水平越高,促进药物排泄的能力越强,肿瘤细胞的耐药性越显著,化疗疗效越差。
文摘目的 分析15例嗜麦芽窄食单胞菌败血症患者的临床因素及菌株同源性.方法 收集2006年9至12月浙江大学医学院附属第一医院肝移植病房的15例嗜麦芽窄食单胞菌败血症患者的临床资料,采用浓度梯度法(etest)测定15株细菌对10种常用抗菌药物的最小抑菌浓度(mic),通过whonet5软件对菌株进行抗生素分型,脉冲场凝胶电泳(pfge)分析菌株同源性.结果 所有患者均有机械通气史和长期使用广谱β-内酰胺类和喹诺酮类药物史,15株嗜麦芽窄食单胞菌在体外对米诺环素、复方磺胺甲恶唑和环丙沙星敏感性最好.抗生素型和pfge分析提示嗜麦芽窄食单胞菌败血症为局部流行,在不同时间段有克隆a(9株)和克隆b(6株)两个克隆株流行.结论 机械通气可能与肝移植患者发生嗜麦芽窄食单胞菌败血症有关.
abstract：
objective to investigate the related clinical factors and homology of strains in stenotrophomonas maltophilia (s. maltophilia) infections in 15 patients with liver transplantation. methods fifteen patients with s. maltophilia infection from september to december 2006 were enrolled and their clinical data were collected. minimal inhibitory concentrations (mics) of 10 antimierobial agents against s. maltophilia were determined by etest strips. antibiogram was carried out by resistance analysis assembly with whonet 5 software. the genomic dna of all the isolates was digested with xbal and subjected to pulse-field gel electrophoresis (pfge). results all patients received mechanical ventilation during the treatment and had a history of long-term use of extended-spectrum β-lactamases and quinolones. mics of 10 antimicrobial agents indicated that s. maltophilia were susceptible to several antimicrobial agents including compound sulfamethoxazole and ciprofloxacin, but the best active agent against these resistant isolates was minocycline in vitro. the results of all 15 s. maltophilia antibiograms were accordance with pfge patterns. all 15 s. maltophilia isolates were classified as 2 pfge patterns: 9 for pattern a and 6 for pattern b. conclusion mechanical ventilation might be associated with the s. maltophilia septicemia in patients with liver transplantation.
