A method of determination by UV spectrophotometry and identification by thin - layer chromatography of tetracaine hydrochloride eye drop was established. The maximum absorbance wavel ength at 310 ± 1nm was select...A method of determination by UV spectrophotometry and identification by thin - layer chromatography of tetracaine hydrochloride eye drop was established. The maximum absorbance wavel ength at 310 ± 1nm was selected for determination wavelength, ethyl hydroxybenzoate has no interferrence. The result showed that the absorbance coeffecient (E1cm1%) of tetracaine hydrochloride at 310 ± 1nm was 760,RSD = 0.5% （n = 20）, the mean recovery for sample was 99.5%, RSD = 0.5% （n = 7）. This method is simple, rapid, accurate and reproducible.展开更多
文摘A method of determination by UV spectrophotometry and identification by thin - layer chromatography of tetracaine hydrochloride eye drop was established. The maximum absorbance wavel ength at 310 ± 1nm was selected for determination wavelength, ethyl hydroxybenzoate has no interferrence. The result showed that the absorbance coeffecient (E1cm1%) of tetracaine hydrochloride at 310 ± 1nm was 760,RSD = 0.5% （n = 20）, the mean recovery for sample was 99.5%, RSD = 0.5% （n = 7）. This method is simple, rapid, accurate and reproducible.
