从患病中华鳖(Pelodiscus sinensis)肺组织中分离到优势菌1株,进行了表型特征分析和16S r DNA序列测定。结果表明,该菌为气单胞菌属的嗜水气单胞菌(Aeromonas hydrophila),通过对健康中华鳖进行人工感染,发现该菌具有致病性;选取20种常...从患病中华鳖(Pelodiscus sinensis)肺组织中分离到优势菌1株,进行了表型特征分析和16S r DNA序列测定。结果表明,该菌为气单胞菌属的嗜水气单胞菌(Aeromonas hydrophila),通过对健康中华鳖进行人工感染,发现该菌具有致病性;选取20种常规药敏试纸进行药敏试验,结果表明,该嗜水气单胞菌对庆大霉素、头孢他啶、头孢西丁、链霉素、呋喃妥因敏感,对诺氟沙星、红霉素、阿米卡星、环丙沙星中敏,对其他11种抗生素具有耐药性。展开更多
2015年3-4月,结合红螯螯虾(Cherax quadricarinatus Von Martens)育苗周期,对温室育苗系统水处理单元的水质指标实施周期监测。结果表明,水源水质的总氮(TN)、总磷(TP)、氨氮(NH3-N)、亚硝酸盐(NO2-N)、硝酸盐(NO3-N)和高锰酸盐指数(COD...2015年3-4月,结合红螯螯虾(Cherax quadricarinatus Von Martens)育苗周期,对温室育苗系统水处理单元的水质指标实施周期监测。结果表明,水源水质的总氮(TN)、总磷(TP)、氨氮(NH3-N)、亚硝酸盐(NO2-N)、硝酸盐(NO3-N)和高锰酸盐指数(CODMn)均较低,符合养殖标准。在育苗期间,育苗池进水口的TN、TP、NO2-N和NO3-N的平均值最高,分别为6.2、0.76、0.035、4.2 mg/L;袋式过滤器的NH3-N浓度最高,平均为0.23 mg/L,各处理单元的CODMn值差异不大。随着育苗时间的延长,育苗水体各指标均有不同程度的升高。从处理单元分析,经处理后,水质指标明显改善,保证了育苗过程的顺利进行。展开更多
The agglutination characteristics of serum agglutinin from Portunus trituberculatus were studied in this paper. The results showed that the serum agglutinin had no agglutination to crucian carp, Chinese soft shell tur...The agglutination characteristics of serum agglutinin from Portunus trituberculatus were studied in this paper. The results showed that the serum agglutinin had no agglutination to crucian carp, Chinese soft shell turtle, grass carp, chicken or human group A, B or O blood cells, but had strong agglutination to blood cells of mice and rabbits. The activities of serum agglutinin from Portunus trituberculatus to mice blood cells reached 210, and to rabbit blood cells reached 28. Salinity had a greater effect on agglutinin activity. When the Na Cl concentration exceeded 0.6 mol/L,the serum agglutinin from Portunus trituberculatus was basically inactivated. The optimum p H for agglutinin activity was 6.0-7.4. The serum agglutinin from Portunus trituberculatus had obvious dependence to Ca2 ＋and Mg2 ＋, and EDTA could significantly inhibit its activity. The results of sugar inhibition test showed that the activity of agglutinin from Portunus trituberculatus can be specifically inhibited by N-acetylglucosamine and N-acetylmannosamine. The serum agglutinin from Portunus trituberculatus was isolated by ammonium sulfate gradient precipitation, and its activity was highest by the 25% ammonium sulfate precipitation system. The SDS polyacrylamide gel electrophoresis（SDS-PAGE） showed that the protein bands were mainly distributed within 72-95 ku.展开更多
文摘从患病中华鳖(Pelodiscus sinensis)肺组织中分离到优势菌1株,进行了表型特征分析和16S r DNA序列测定。结果表明,该菌为气单胞菌属的嗜水气单胞菌(Aeromonas hydrophila),通过对健康中华鳖进行人工感染,发现该菌具有致病性;选取20种常规药敏试纸进行药敏试验,结果表明,该嗜水气单胞菌对庆大霉素、头孢他啶、头孢西丁、链霉素、呋喃妥因敏感,对诺氟沙星、红霉素、阿米卡星、环丙沙星中敏,对其他11种抗生素具有耐药性。
基金Supported by National Natural Science Foundation of China(41106123)National High Technology Research and Development Program of China(863 Program)(2012AA10A409)+1 种基金Natural Science Foundation of Zhejiang Province(LY12C19009)Key Special Project of Department of Science and Technology of Zhejiang Province(2012C12907-3)
文摘The agglutination characteristics of serum agglutinin from Portunus trituberculatus were studied in this paper. The results showed that the serum agglutinin had no agglutination to crucian carp, Chinese soft shell turtle, grass carp, chicken or human group A, B or O blood cells, but had strong agglutination to blood cells of mice and rabbits. The activities of serum agglutinin from Portunus trituberculatus to mice blood cells reached 210, and to rabbit blood cells reached 28. Salinity had a greater effect on agglutinin activity. When the Na Cl concentration exceeded 0.6 mol/L,the serum agglutinin from Portunus trituberculatus was basically inactivated. The optimum p H for agglutinin activity was 6.0-7.4. The serum agglutinin from Portunus trituberculatus had obvious dependence to Ca2 ＋and Mg2 ＋, and EDTA could significantly inhibit its activity. The results of sugar inhibition test showed that the activity of agglutinin from Portunus trituberculatus can be specifically inhibited by N-acetylglucosamine and N-acetylmannosamine. The serum agglutinin from Portunus trituberculatus was isolated by ammonium sulfate gradient precipitation, and its activity was highest by the 25% ammonium sulfate precipitation system. The SDS polyacrylamide gel electrophoresis（SDS-PAGE） showed that the protein bands were mainly distributed within 72-95 ku.
