目的研究苦丁茶对自发性高血压大鼠心室重构的影响。方法将实验大鼠随机分为SHR苦丁茶处理组、SHR空白组以及WKY对照组,实验8周后观察大鼠血压、心肌厚度、LVWI值、CVF值、PVCA值、H-E和Masson染色病理学情况以及心肌Adipo R1 m RNA与Ad...目的研究苦丁茶对自发性高血压大鼠心室重构的影响。方法将实验大鼠随机分为SHR苦丁茶处理组、SHR空白组以及WKY对照组,实验8周后观察大鼠血压、心肌厚度、LVWI值、CVF值、PVCA值、H-E和Masson染色病理学情况以及心肌Adipo R1 m RNA与Adipo R1蛋白表达情况。结果苦丁茶作用8周后,SHR苦丁茶处理组大鼠收缩压、左心室厚度、LVWI值、CVF值和PVCA值均低于SHR空白组,差异具有统计学意义(P<0.05)。病理学改变上SHR苦丁茶处理组心肌纤维化程度低于SHR空白组。SHR苦丁茶处理组大鼠Adipo R1 m RNA和Adipo R1蛋白相对表达量相比SHR空白组明显增加,差异具有统计学意义(P<0.05)。结论苦丁茶可以抑制自发性高血压大鼠心肌重构。展开更多
目的观察银杏叶提取物对大鼠内耳组织及蜗神经核线粒体(mt)DNA4834bp缺失突变的影响。方法取24月龄SD大鼠20只,随机分为实验组和对照组,各10只,分别用银杏叶提取物100mg/(kg·d)、等体积生理盐水灌胃,3个月后处死。干预前后分别测...目的观察银杏叶提取物对大鼠内耳组织及蜗神经核线粒体(mt)DNA4834bp缺失突变的影响。方法取24月龄SD大鼠20只,随机分为实验组和对照组,各10只,分别用银杏叶提取物100mg/(kg·d)、等体积生理盐水灌胃,3个月后处死。干预前后分别测试两组大鼠ABR,荧光定量PCR检测线粒体DNA4834bp缺失率,并观察其与老年听力损失的关系。结果干预后实验组ABR阈移(3.91±3.73)d B pe SPL,对照组ABR阈移(9.64±2.59)d B pe SPL,两组比较,差异有统计学意义(P<0.05)。与对照组比较,实验组蜗神经核、内耳组织线粒体DNA4834bp缺失率降低(0.2466±0.1335比1.0461±0.3423,0.3507±0.158比1.0202±0.2269,P<0.05)。结论银杏叶提取物能抑制大鼠听觉器官线粒体DNA4834bp缺失突变,减少mt DNA的氧化损伤,改善线粒体功能,改善听力,从而延缓大鼠老年性耳聋进展。展开更多
目的 通过电子显微镜观察慢性鼻-鼻窦炎患者手术黏膜样本是否存在细菌生物膜及其形态学特征,分析细菌生物膜形成与临床因素的相关性.方法 实验组获取69例慢性鼻-鼻窦炎患者手术中筛窦黏膜样本,对照组获取15例鼻中隔偏曲患者、10例鼻骨...目的 通过电子显微镜观察慢性鼻-鼻窦炎患者手术黏膜样本是否存在细菌生物膜及其形态学特征,分析细菌生物膜形成与临床因素的相关性.方法 实验组获取69例慢性鼻-鼻窦炎患者手术中筛窦黏膜样本,对照组获取15例鼻中隔偏曲患者、10例鼻骨骨折患者钩突黏膜样本,所有样本均行扫描电子显微镜检查.以crs患者常见的临床表现为调查条目,借鉴鼻窦炎专用量表—鼻腔鼻窦结局测试20的等级描述形式,对患者进行术前临床因素评估.应用spss 10.0软件进行统计学分析,细菌生物膜形成与临床因素的相关性分析采用卡方检验.结果 实验组样本发现细菌生物膜的病例49例,阳性率为71.0％(49/69).所有实验组样本均观察到黏膜纤毛不同程度的损伤.对照组样本未发现细菌生物膜,可以观察到黏膜纤毛较浓密,排列整齐.卡方检验结果表明细菌生物膜阴性组与阳性组在性别、疾病分期、病程,以及鼻塞、流涕、鼻涕臭味、头痛、涕血、嗅觉减退等临床症状方面差异无统计学意义.结论 慢性鼻-鼻窦炎患者黏膜可以观察到不同生命周期的细菌生物膜及黏膜纤毛损伤,细菌生物膜参与慢性鼻-鼻窦炎的发病过程.细菌生物膜形成与各临床症状无相关性.
abstract：
objective to observe the bacterial biofilm in patients with chronic rhinosinusitis (crs), and to investigate the possible relationship between biofilm and clinical factors. methods sixtynine patients with crs ( study group), 15 patients with nasal septum deviation and 10 patients with nasal bone fracture (control group) were enrolled in the study. mucosa specimens of uncinate process or ethmoid near the ostium of the maxillary sinus were obtained during endoscopic sinus surgery. the specimens were subjected to scanning electron microscopy. all patients were evaluated by questionnaire of chnical factors based on sino-nasal outcome test-20. spss 10. 0 was used for statistical analysis, and the relationship between bacterial biofilm and clinical factors was evaluated by chi-square test. results bacterial biofilms were found in 49 patients ( 71.0％ ) with crs. a marked destruction of the epithelium and cilia was observed in all samples of study group. no bacterial biofilm was found in the control group, and scanning electron microscopy showed normal epithelium and cilia in those specimens. chi-square test showed that bacterial biofilm was not associated with clinical factors (gender, staging, course, nasal obstruction, phlegm, nasal discharge with stinking smell, headache, bloody nasal discharge and olfactory degeneration) in crs. conclusions bacterial biofilms and destruction of the epithelium and cilia can be obscrved in crs patients, which may be involved in the pathogenesis of crs, but the formation of bacterial biofilm is not correlated with the clinical factors in crs.展开更多
文摘目的研究苦丁茶对自发性高血压大鼠心室重构的影响。方法将实验大鼠随机分为SHR苦丁茶处理组、SHR空白组以及WKY对照组,实验8周后观察大鼠血压、心肌厚度、LVWI值、CVF值、PVCA值、H-E和Masson染色病理学情况以及心肌Adipo R1 m RNA与Adipo R1蛋白表达情况。结果苦丁茶作用8周后,SHR苦丁茶处理组大鼠收缩压、左心室厚度、LVWI值、CVF值和PVCA值均低于SHR空白组,差异具有统计学意义(P<0.05)。病理学改变上SHR苦丁茶处理组心肌纤维化程度低于SHR空白组。SHR苦丁茶处理组大鼠Adipo R1 m RNA和Adipo R1蛋白相对表达量相比SHR空白组明显增加,差异具有统计学意义(P<0.05)。结论苦丁茶可以抑制自发性高血压大鼠心肌重构。
文摘目的观察银杏叶提取物对大鼠内耳组织及蜗神经核线粒体(mt)DNA4834bp缺失突变的影响。方法取24月龄SD大鼠20只,随机分为实验组和对照组,各10只,分别用银杏叶提取物100mg/(kg·d)、等体积生理盐水灌胃,3个月后处死。干预前后分别测试两组大鼠ABR,荧光定量PCR检测线粒体DNA4834bp缺失率,并观察其与老年听力损失的关系。结果干预后实验组ABR阈移(3.91±3.73)d B pe SPL,对照组ABR阈移(9.64±2.59)d B pe SPL,两组比较,差异有统计学意义(P<0.05)。与对照组比较,实验组蜗神经核、内耳组织线粒体DNA4834bp缺失率降低(0.2466±0.1335比1.0461±0.3423,0.3507±0.158比1.0202±0.2269,P<0.05)。结论银杏叶提取物能抑制大鼠听觉器官线粒体DNA4834bp缺失突变,减少mt DNA的氧化损伤,改善线粒体功能,改善听力,从而延缓大鼠老年性耳聋进展。
文摘目的 通过电子显微镜观察慢性鼻-鼻窦炎患者手术黏膜样本是否存在细菌生物膜及其形态学特征,分析细菌生物膜形成与临床因素的相关性.方法 实验组获取69例慢性鼻-鼻窦炎患者手术中筛窦黏膜样本,对照组获取15例鼻中隔偏曲患者、10例鼻骨骨折患者钩突黏膜样本,所有样本均行扫描电子显微镜检查.以crs患者常见的临床表现为调查条目,借鉴鼻窦炎专用量表—鼻腔鼻窦结局测试20的等级描述形式,对患者进行术前临床因素评估.应用spss 10.0软件进行统计学分析,细菌生物膜形成与临床因素的相关性分析采用卡方检验.结果 实验组样本发现细菌生物膜的病例49例,阳性率为71.0％(49/69).所有实验组样本均观察到黏膜纤毛不同程度的损伤.对照组样本未发现细菌生物膜,可以观察到黏膜纤毛较浓密,排列整齐.卡方检验结果表明细菌生物膜阴性组与阳性组在性别、疾病分期、病程,以及鼻塞、流涕、鼻涕臭味、头痛、涕血、嗅觉减退等临床症状方面差异无统计学意义.结论 慢性鼻-鼻窦炎患者黏膜可以观察到不同生命周期的细菌生物膜及黏膜纤毛损伤,细菌生物膜参与慢性鼻-鼻窦炎的发病过程.细菌生物膜形成与各临床症状无相关性.
abstract：
objective to observe the bacterial biofilm in patients with chronic rhinosinusitis (crs), and to investigate the possible relationship between biofilm and clinical factors. methods sixtynine patients with crs ( study group), 15 patients with nasal septum deviation and 10 patients with nasal bone fracture (control group) were enrolled in the study. mucosa specimens of uncinate process or ethmoid near the ostium of the maxillary sinus were obtained during endoscopic sinus surgery. the specimens were subjected to scanning electron microscopy. all patients were evaluated by questionnaire of chnical factors based on sino-nasal outcome test-20. spss 10. 0 was used for statistical analysis, and the relationship between bacterial biofilm and clinical factors was evaluated by chi-square test. results bacterial biofilms were found in 49 patients ( 71.0％ ) with crs. a marked destruction of the epithelium and cilia was observed in all samples of study group. no bacterial biofilm was found in the control group, and scanning electron microscopy showed normal epithelium and cilia in those specimens. chi-square test showed that bacterial biofilm was not associated with clinical factors (gender, staging, course, nasal obstruction, phlegm, nasal discharge with stinking smell, headache, bloody nasal discharge and olfactory degeneration) in crs. conclusions bacterial biofilms and destruction of the epithelium and cilia can be obscrved in crs patients, which may be involved in the pathogenesis of crs, but the formation of bacterial biofilm is not correlated with the clinical factors in crs.
