Crude fucoidanase was extracted from digestive organ of a herbivorous marine gastropod LuneZla cornata coreensis. The characteristics of fucoidanase dynamics e.g. optimum pH,optimum temperature, activators, inhibitors...Crude fucoidanase was extracted from digestive organ of a herbivorous marine gastropod LuneZla cornata coreensis. The characteristics of fucoidanase dynamics e.g. optimum pH,optimum temperature, activators, inhibitors, Michaelis constant(Km) and maximum velocity (Vmax) were assayed. According to these characteristics, two kinds of fucoidanase defined as fucoidanase A and B might exist in the crude extract. Purification of fueoidanase was carried out by the method of ammonium sulfate-precipitation and the enzymic preparation of acetone powder was made.展开更多
Since the successful cloning of the female and male gametophytes of Laminaria japonica in our lab., a new breed “Danhai No.1” derived from a female cell clone b, as been selected and used in the kelp industrial cult...Since the successful cloning of the female and male gametophytes of Laminaria japonica in our lab., a new breed “Danhai No.1” derived from a female cell clone b, as been selected and used in the kelp industrial cultivation. Plans to produce hybrid Laminaria have been put into practice for some years.It was to use the clones of different female and male gametophytes for various crosses. After trials, several laminarian hybrids (F1) have been obtained. The hybrid breed “Danza No.10” has been found to be the best, The clones used were FH10 and MHJ. Here FH10 stands for the female haploid clone No.10 and MHJ stands for the male haploid cell clone introduced from Hokkaido, Japan in 1981. The pedigree of the hybrid is as follows: FH10×MHJ→“Danza No.10” When the hybrid was selffertilized it gave broad segregation in the F2, as expected. When the hybrid was compared with the control in productivity it showed superiority. It was estimated to be able to increase 30% yield over the control which is a high productive breed widely cultivated here. The iodine content of the hybrid was rather high too, more than 70‰. Obviously it is much easier to use the Laminaria hybrid in mariculture than to use hybrid rice in agriculture. No male sterility is needed.The main thing is to keep the two cell clones (Which would give rise to F1 heterosis) vigorously in the lab or in the green house.展开更多
鲈鱼Lateolabrax japonicus(C et V)为近岸浅海习见的广盐性鱼类喜栖息河口咸淡水处，亦可生活淡水中。海、淡两种不同盐度和渗透压必然导致肾结构及其功能的变化(M·E勃朗1962；邵炳绪1981)。但对鲈鱼肾脏的显微与亚微结构情况至...鲈鱼Lateolabrax japonicus(C et V)为近岸浅海习见的广盐性鱼类喜栖息河口咸淡水处，亦可生活淡水中。海、淡两种不同盐度和渗透压必然导致肾结构及其功能的变化(M·E勃朗1962；邵炳绪1981)。但对鲈鱼肾脏的显微与亚微结构情况至今未见报道。为此本文分别取用海产和淡养两种不同生境中生长的鲈鱼肾脏，进行光镜和电镜观察、比较，并对其出现的结构变化作了初步研究。展开更多
In this paper, the composition, distribution, seasonal variation and biomass of benthic algae of Qingdao Mati Reef were studied during the period from May of 1981 to April of 1982.The results of the investigation are ...In this paper, the composition, distribution, seasonal variation and biomass of benthic algae of Qingdao Mati Reef were studied during the period from May of 1981 to April of 1982.The results of the investigation are as follows: 1. 83 species of benthic algae of Qingdao Mati Reef were found,and they are indentified as: 13 Chlorophyta, 16 Phaeophyta and 54 Rhodo-phyta.2. According to the flora of 83 species of benthic algae in Qingdao Mati Reef, it seems that they belong to warm temperate.3. The annual biomass and biomass variation of benthic algae in March were hightest of the west part of Qingdao Mati Reef.展开更多
文摘Crude fucoidanase was extracted from digestive organ of a herbivorous marine gastropod LuneZla cornata coreensis. The characteristics of fucoidanase dynamics e.g. optimum pH,optimum temperature, activators, inhibitors, Michaelis constant(Km) and maximum velocity (Vmax) were assayed. According to these characteristics, two kinds of fucoidanase defined as fucoidanase A and B might exist in the crude extract. Purification of fueoidanase was carried out by the method of ammonium sulfate-precipitation and the enzymic preparation of acetone powder was made.
文摘Since the successful cloning of the female and male gametophytes of Laminaria japonica in our lab., a new breed “Danhai No.1” derived from a female cell clone b, as been selected and used in the kelp industrial cultivation. Plans to produce hybrid Laminaria have been put into practice for some years.It was to use the clones of different female and male gametophytes for various crosses. After trials, several laminarian hybrids (F1) have been obtained. The hybrid breed “Danza No.10” has been found to be the best, The clones used were FH10 and MHJ. Here FH10 stands for the female haploid clone No.10 and MHJ stands for the male haploid cell clone introduced from Hokkaido, Japan in 1981. The pedigree of the hybrid is as follows: FH10×MHJ→“Danza No.10” When the hybrid was selffertilized it gave broad segregation in the F2, as expected. When the hybrid was compared with the control in productivity it showed superiority. It was estimated to be able to increase 30% yield over the control which is a high productive breed widely cultivated here. The iodine content of the hybrid was rather high too, more than 70‰. Obviously it is much easier to use the Laminaria hybrid in mariculture than to use hybrid rice in agriculture. No male sterility is needed.The main thing is to keep the two cell clones (Which would give rise to F1 heterosis) vigorously in the lab or in the green house.
文摘鲈鱼Lateolabrax japonicus(C et V)为近岸浅海习见的广盐性鱼类喜栖息河口咸淡水处，亦可生活淡水中。海、淡两种不同盐度和渗透压必然导致肾结构及其功能的变化(M·E勃朗1962；邵炳绪1981)。但对鲈鱼肾脏的显微与亚微结构情况至今未见报道。为此本文分别取用海产和淡养两种不同生境中生长的鲈鱼肾脏，进行光镜和电镜观察、比较，并对其出现的结构变化作了初步研究。
文摘In this paper, the composition, distribution, seasonal variation and biomass of benthic algae of Qingdao Mati Reef were studied during the period from May of 1981 to April of 1982.The results of the investigation are as follows: 1. 83 species of benthic algae of Qingdao Mati Reef were found,and they are indentified as: 13 Chlorophyta, 16 Phaeophyta and 54 Rhodo-phyta.2. According to the flora of 83 species of benthic algae in Qingdao Mati Reef, it seems that they belong to warm temperate.3. The annual biomass and biomass variation of benthic algae in March were hightest of the west part of Qingdao Mati Reef.
