DA novel chitinase gene (GhChia7) was isolated from salicylic acid (SA)-treated cotton cotyledons and characterized by DNA sequence analysis of its cDNA and genomic DNA clone. The deduced amino acid sequence, designat...DA novel chitinase gene (GhChia7) was isolated from salicylic acid (SA)-treated cotton cotyledons and characterized by DNA sequence analysis of its cDNA and genomic DNA clone. The deduced amino acid sequence, designated as class VII chitinase, shares about 30% identity to class I or II chitinases, and does not correspond to any of the previously characterized classes I-VI chitinases. Northern blotting analysis showed that the transcripts of GhChia7 were abundant both in cotton fibers and in the roots of the seedlings. The accumulation of GhChia7 mRNA in SA-treated cotyledons reached maximum at 7.5 mmol/ L concentration after 18 h. Results indicate that GhChia7 might play an important role in cotton's active defense response.展开更多
文摘DA novel chitinase gene (GhChia7) was isolated from salicylic acid (SA)-treated cotton cotyledons and characterized by DNA sequence analysis of its cDNA and genomic DNA clone. The deduced amino acid sequence, designated as class VII chitinase, shares about 30% identity to class I or II chitinases, and does not correspond to any of the previously characterized classes I-VI chitinases. Northern blotting analysis showed that the transcripts of GhChia7 were abundant both in cotton fibers and in the roots of the seedlings. The accumulation of GhChia7 mRNA in SA-treated cotyledons reached maximum at 7.5 mmol/ L concentration after 18 h. Results indicate that GhChia7 might play an important role in cotton's active defense response.
