Mechanisms and Research Progress of Traditional Chinese Medicine Regulating NF-κB in the Treatment of Acute Lung Injury/Acute Respiratory Distress Syndrome

Acute lung injury(ALI)has multiple causes and can easily progress to acute respiratory distress syndrome(ARDS)if not properly treated.Nuclear factorκB(NF-κB)is a key pathway in the treatment of ALI/ARDS.By exploring the relevance of NF-κB and the pathogenesis of this disease,it was found that this disease was mainly associated with inflammation,dysfunction of the endothelial barrier,oxidative stress,impaired clearance of alveolar fluid,and coagulation disorders.Traditional Chinese medicine(TCM)has the characteristics of multitargeting,multipathway effects,and high safety,which can directly or indirectly affect the treatment of ALI/ARDS.This article summarizes the mechanism and treatment strategies of TCM in recent years through intervention in the NF-κB-related signaling pathways for treating ALI/ARDS.It provides an overview from the perspectives of Chinese herbal monomers,TCM couplet medicines,TCM injections,Chinese herbal compounds,and Chinese herbal preparations,offering insights into the prevention and treatment of ALI/ARDS with TCM.

卟啉化合物PN—5 Fu的光动力学治疗作用机理初步研究

小鼠腹水癌细胞经PN-5Fu加光辐照处理后,癌细胞脂质过氧化产物丙二醛(MDA)、形成增多,随光照时间延长,其形成量显著增加(P>0.05-0.01),荷瘤小鼠局部肿瘤经PN-5Fu光动力治疗后,肿瘤组织中MDA含量也明显高于对照纽。(P<0.05),提示PN-5Fu光化学反应可以引起癌细胞膜磷脂中多不饱和脂肪酸的脂质过氧化。用苯甲酸捕获·OH的荧光法测定表明,PN-5Fu光动力反应过程中有羟自由基生成。荧光偏振方法测定细胞膜脂流动性的结果显示PN-5Fu的光动学作用可使腹水癌细胞膜的微粘度明显增高,反映膜脂的流动性明显下降。由透射电镜观察到PN-5Fu光动力治疗作用可使癌细胞超微结构发生显著变化,光照1小时后癌细胞胞浆疏松,线粒体肿胀、空泡变性;光照6小时后可见癌细胞膜破坏,核浓缩、碎解、甚至捎失。实验还表明,PN-5Fu光动学作用所致皮肤光敏反应可完全被维生素E所阻断,苯海拉明及消炎痛均能对抗PN-5Fu的皮肤光敏反应。

Acupuncture and Related Therapies for Hyperlipidemia:A Network Meta-Analysis

Objective To compare and rank the clinical effects of different acupuncture and related therapies on hyperlipidemia patients.Methods We used network meta-analysis(NMA)to evaluate direct and indirect effects in studies of acupuncture and related therapies for hyperlipidemia.Databases PubMed,EMBASE,Cochrane Library,the China Biology Medicine(CBM),the China National Knowledge Infrastructure(CNKI),Wanfang Data and the Chinese Scientific Journal Database(VIP)were searched to collect randomized controlled trials(RCTs)of acupuncture and related therapies in the treatment of hyperlipidemia.The data were analyzed using Stata 15.0 and WinBUGS 1.4.3 software after two researchers independently screened the literature,extracted the data,and assessed the risk of bias in the included studies.Results We analyzed a total of 36 eligible studies that included 3124 patients,involving 12 types of acupuncture and related therapies and comprehensive therapies.The results of the NMA showed that:for the total cholesterol(TC),acupoint catgut embedding(ACE),simple acupuncture(ACU),acupoint injection(AI),electroacupuncture(EA),western medicine of statins(WM),and combination of acupuncture and related therapies(combined therapies)were all more effective than placebo(P<0.05).For triacylglycerol(TG),ACU,EA,warming acupuncture(WA),WM and combined therapies were better than placebo(P<0.05),while WA was better than Chinese herb(CH)(P<0.05).For low-density lipoprotein cholesterol(LDL-C),combined therapies were more effective than lifestyle modification(LM)(P<0.05).For high-density lipoprotein cholesterol(HDL-C),auricular acupoint stimulation(AAS),ACE,ACU,AI,CH,EA,LM,moxibustion(MOX),WM,combined therapies and placebo were all worse than WA(P<0.05),while WM and combined therapies were better than ACU(P<0.05).Combined ranking results suggest that ACU and combined therapies may be the optimal intervention.Conclusions The efficacy of all kinds of acupuncture-related therapies in patients with hyperlipidemia is better than lifestyle changes.However,for different outcome indicators,all kinds of acupuncture-related therapies have their advantages and disadvantages,and comprehensive ranking results suggest that ACU and combined therapies may be the optimal intervention.

Modified green dragon swaying its tail needling manipulation for treatment of knee osteoarthritis

Objective To compare the difference in the effect of modified green dragon swaying its tail needling manipulation and common needling manipulations on the overall rating of pain of patients with knee osteoarthritis, and to evaluate the advantages of modified green dragon swaying its tail needling manipulation for. Methods Ninety-five patients with knee osteoarthritis were divided into 2 groups according to the random number table method with 48 patients in the modified green dragon swaying its tail group（group special manipulation, SM group for short） and 47 patients in the common acupuncture group（group convention needling, CN group for short）, and different acupuncture manipulations were applied in the two groups. Internationally recognized Simplified Mc Gill Pain Questionnaire（SF-MPQ） scale used for description and measurement of pain was applied to evaluate the pain degree, including pain rating index（PRI）, visnal analogue scale（VAS）and present pain intensity（PPI）. The index of severity for osteoarthritis（ISOA） was adopted for evaluation of clinical efficacy. Results（1） In SM group, the scores of PRI, VAS and PPI were 6.63±4.67, 2.23±1.45 and 0.65±1.32, respectively, after treatment, and in CN group, the scores of PRI, VAS and PPI were 13.32±7.96, 4.34±1.79 and 2.28±1.21, respectively, the scores decreased in the two groups after treatment（all P〈0.05）, and the improvement in SM group was superior to that in CN group（all P〈0.01）.（2） The clinical cure rate in SM group was 52.08%, and the total effective rate was 97.92%, the clinical cure rate in CN group was 25.53%, and the total effective rate was 85.10%. The comparison of clinical cure rate in the two groups showed that P〈0.01. Conclusion The SM group is obviously superior to CN group in the improvement of overall rating of pain of patients. It is indicated that modified green dragon swaying its tail acupuncture therapy can effectively relieve the pain of patients with knee osteoarthritis, which was worthy of popularization and application with definite clinical efficacy

Analysis of the prescription of auricular acupoint therapy for simple obesity based on complex network techniques

Objective： To explore the core acupoints and combination rules of auricular acupoint therapy for simple obesity, and to further analyze the characteristics of the prescription of auricular acupoint therapy for simple obesity.Methods： Relevant clinical study literature in recent 30 years in PubMed, China Biology Medicine disc（CBM）, China National Knowledge Infrastructure（CNKI）, Wan Fang Database. VIP Database and TCM Online Database was retrieved, and eligible articles were selected in order to build a prescription database of auricular acupoint therapy for simple obesity. On the basis of complex network techniques, the core acupoints and combination rules of auricular acupoint therapy for simple obesity were analyzed, and the characteristics of auricular acupoint therapy for simple obesity were analyzed comprehensively.Results： There were 46 network nodes of auricular acupoint. The top 16 core acupoints for auricular acupoint therapy for simple obesity included Nèifēnmì（内分泌CO18）, Pí（脾CO13）, Wèi（胃CO4）, Sānjiāo（三焦CO17）, Jīdiǎn（饥点）.Shénmén（神门TF4）. Dàcháng（大肠CO7）. Pízhìxià（皮质下AT4）. Fèi（肺CO14）. Shèn（肾CO10）. Jiāogǎn（交感AH6 a）, Kǒu（口CO1）,Gān（肝CO12）. Xiǎocháng（小肠CO6） and Nǎo（脑）. The combination of auricular acupoints was mainly based on the main indications of acupoints. The analysis of auricular acupoints combination indicated that the combination of CO4 with CO18 was applied most frequently, which was followed by the combinations of CO13 with CO18 and CO13 with C04. According to the analysis of auricular acupoint stimulation methods, ear point taping and pressing with Wángbùliúxíng（王不留行,Semen Vaccariae） seeds was used frequently, which was followed by magnetic beads taping and pressing and pyonex therapy. Auricular acupoint therapy combined with acupuncture for simple obesity was used most commonly, which was followed by auricular acupoint therapy combined with catgut embedment in acupoint and simple auricular acupoint therapy.Conclusion： In this study, the core acupoints and combinations of auricular acupoint therapy for simple obesity were explored effectively, and the pressing materials and major combined intervention methods were summarized and analyzed, thus providing references and treatment thoughts in terms of the point and prescription selection of auricular acupoint therapy for simple obesity.

A new alkaloid from Chaetomium globosum

A new alkaloid, methyl 6-（isoprenyl）-lH-indole-3-carboxylate （1）, was isolated from the solid culture broth of fungus Chaetomium globosum, together with five known compounds, including N-acetyl-L-tryptophan （2）, xylariol B （3）, 5-（methoxymethyl）- 1H-pyrrole-2-carbaldehyde （4）, ergosterol （5） and ergosta-4,6,8（14）,22-tetraen-3-one （6）. Their chemical structures were elucidated by spectroscopic data （UV, IR, HRESIMS, and NMR）. In addition, it is the first report of compounds 2 and 3 from the fungus Chaetomium globosum.