Curcuminoids Inhibit Botrytis cinerea and Colletotrichum gloeosporioides

The growth rate method was adopted to measure the inhibitory effect of curcumin,tetrahydrocurcumin,demethoxycurcumin,and bisdemethoxycurcumin on the mycelial growth of Botrytis cinerea and Colletotrichum gloeosporioides.The results showed that the four curcuminoids inhibited the mycelial growth of the two pathogens in a concentration-dependent manner.Bisdemethoxycurcumin at 600 mg/L exerted the strongest inhibitory effect on the mycelial growth of B.cinerea and C.gloeosporioides,with the relative inhibition rates of 98.19%and 100%,respectively;followed by demethoxycurcumin;curcumin exerted the worst inhibitory effect.Toxicity test results also showed that four curcuminoids all had a certain toxicity to B.cinerea and C.gloeosporioides,among which,bisdemethoxycurcumin exhibited the strongest toxicity,with the EC_(50)of 131.125 and 122.235 mg/L,respectively;while curcumin had the lowest toxicity,with the EC_(50)of 273.143 and 194.943 mg/L,respectively.

Cloning of BjNAC102 Promoter and Construction of Expression Vector in Brassica juncea

Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea‘Sichuan Yellow Seed’by using the homologous cloning method.The expression vector of the GUS gene driven by the BjNAC102 promoter was constructed by seamless cloning technology.The results showed that the sequence of the promoter of the BjNAC102 gene contained many cis-acting elements involved in light responsiveness,gibberellinresponsive element,and auxin-responsive element.It was speculated that BjNAC102 played an important role in the abiotic stress response in Brassica juncea.The expression vector of the promoter of the BjNAC102 gene was constructed,which layed a foundation for further studies of the expression pattern of the BjNAC102 gene in Brassica juncea.

OBE教学模式下"数控车削编程与加工"课程改革浅探

文章分析"数控车削编程与加工"传统教学模式存在的问题,运用OBE教学模式从制定课程目标、定义学习成果、架构课程内容、有效实施教学、评价学习成果、创设保障条件六方面探索"数控车削编程与加工"课程改革,为下一步构建本土化OBE课程教学评价标准提供实践基础.

Control Effects of Mixture of Metamifop and Cyhalofop-butyl on Annual Weeds Barnyard Grass in Direct-seeding Paddy Field

In order to explore the control effects of 10% metamifop EC and 20%cyhalofop-butyl OD on barnyard grass(Echinochloa crusgalli(L.) Beauv.) as well as their safety to rice in rice direct-seeding paddy field, field efficacy experiments were carried out in Taoyuan County and Hanshou County of Hunan Province. The results showed that the control effect of different pesticide treatments on barnyard grass was good, and the control effect reached more than 93%. Of which, 10% metamifop EC 2 250 mL/hm^(2)+20% cyhalofop-butyl OD 1 500 mL/hm^(2)had the best control effect,exceeding 99% and each treatment had no significantly ponisonous to rice. However,considering cost-saving and effect-increasing, it was recommended to use 10%metamifop EC 1 050 mL/hm^(2)mixed with 20% cyhalofop-butyl OD 1 050 mL/hm^(2)for the control of barnyard grass, which not only had the effect of controlling barnyard grass, but also was safety to rice.

Analysis on Genetic Differences between Parents of Two-line Medium Indica Hybrid Rice by SSR Markers

The genetic diversity of 50 parents or improved lines of two-line medium Indica hybrid rice were analyzed based on 48 pairs of SSR markers. The results showed that the clustering results were highly accordant with the parental pedigree information of the tested materials. The male sterile lines and the restoring lines were clustered into 2 distinct groups （Ⅰ and Ⅱ）, respectively. There were no significant difference in intra-group average genetic distances （GD） between the 2 groups. Most of the male sterile lines from Hunan Longping High-tech Seed Science Research Institute Co., Ltd. were grouped into a single subgroup （Ⅰ-4） under the male sterile group with the largest average GD compared with the rest male sterile subgroups. Huazhan derived lines and Guangzhou inbred lines were founded to be independent of 9311 and its derivatives or closely-related lines. Meanwhile, they have obviously been differentiated into 2 subgroups （Ⅱ-2 and Ⅱ-3）. The lines derived from Huazhan （or genetic closely related lines） showed a genetic homogenization phenomenon. Therefore, the Huazhan-derived lines should be further improved by the utilization of germplasms with good quality, high resistance and wide adaptability from Southern China to widen the GD between Huazhan derived lines and the male sterile subgroup developed by Hunan Longping High-tech Seed Science Research Institute Co., Ltd., so as to further promote the heterosis level between the groups.

事业单位改革与人事档案管理创新

作为服务社会的重要组织，事业单位的体制改革正在深入进行，人事档案管理作为事业单位管理工作的重要组成，通过资料和文件的记录，起到发展和组织人才的重要作用。在新的发展时期，人事档案管理创新成为形势所趋。文章围绕事业单位体制改革，对人事档案管理创新进行分析，寻找有效途径，推动事业单位的发展有着重要的意义。

浅谈现代舞编舞技法在民族民间舞当中的运用

随着时代的不断发展,具有中国地域特色的各民族舞蹈都在不断突破创新.现代舞作为一种表达世界新角度的舞蹈流派,在编舞技法上有很多方面都值得运用到民族民间舞当中,为民族民间舞汇入新鲜的血液,使其更符合现代人们的欣赏水平,不段与时俱进.运用现代舞新的编舞技法,同时深深扎根于优秀的民族文化,抓住民族精神的根基和民族舞蹈的风格特点更好的传承和发扬民族民间舞蹈.脚踏实地地走出宽广的民族民间舞创作之路,弘扬民族民间舞的优秀文化.

A Viral Expression Vector from Foxtail mosaic virus to Express Green Fluorescent Protein

[Objective]Foxtail mosaic virus(FoMV)infects gramineous and dicotyledonous plants.In this study,we sought to construct a viral vector based on FoMV to express exogenous proteins in plants.[Method]A recombinant viral expression vector was constructed by inserting the promotor of Potato virus X(PVX)and exogenous gene sequences into the 3’non-coding region of the FoMV coat protein gene.[Results]The plasmid pCB301-FoMV-CP-PVXprom-GFP expressed green fluorescent protein in inoculated Nicotiana benthamiana leaves.[Conclusion]A recombinant viral expression vector was constructed successfully.

Application of Uniform Design and SVR for Culture Medium Optimization of Organophosphorus Degradation Bacteria

In order to improve the degradation activity of organophosphorus degradation bacterial strains, uniform design and support vector regression(SVR) are used to optimize the formula of mineral salt medium for the strains based on single factor experiment. The formula being used after SVR optimization is glucose 1.24 g/L,NH4 NO3 0.73 g, KH2 PO4 0.96 g, KCl 0.50 g, MgSO4·7 H2 O 0.50 g, MnSO4 0.02 g, CaCl2 0.04 g, distilled water 1 000 mL;the highest degradation rate is obtained at pH7.0(SVR, 76.68%), superior to that of the QSAR model(74.67%). The formula optimization method based on uniform design and SVR is very promising to apply in various multiple-factor and multiple-level formula optimization experiments.

TIB for Micropropagation and the Relationship between Anthocyanins and Chlorophyll of Strawberry Seedlings

In this study, we employed a temporary immersion bioreactor(TIB)system for the micropropagation of strawberry seedlings. The TIB method and the conventional method for the micropropagation of strawberry seedlings were compared in terms of explant propagation coefficient, seedling fresh weight,contents of anthocyanins and chlorophyll, and photosynthetic characteristics. The results showed that an inoculation density of 40 explants/L was suitable for the micropropagation of ’Benihope’ strawberry seedlings in the TIB. The propagation coefficient, fresh weight, photosynthetic rate, stomatal conductance, transpiration coefficient, and the contents of total anthocyanins and chlorophyll of seedlings micropropagated in the TIB were significantly higher than those of the seedlings micropropagated by the conventional method. In conclusion, the TIB method was superior to the conventional method in the micropropagation of strawberry seedlings.The negative reciprocity did not occur between anthocyanins and chlorophyll in strawberry seedlings cultured under forced ventilation such as TIB.

国产手机如何开展品牌战略？

“对于市场盛传重组过后的夏新电子将选择手机品牌及业务外包这一运营订式，业内人士也认为，公司的前途却并非想象中那么乐观”（《夏新山穷水尽难重组手机市场今非昔比能否浴血重生》．“公司”。2009年10月20日）．

论一个“毒霸”的倒掉

“然而一案未了，一案又起。瑞星公司董事长王莘与瑞星公司前总经理、东方微点总经理刘旭两人的二人转正演绎得如火如荼：王莘通过瑞星系列关联公司间的交易腾移资产，从而不断稀释刘旭手中持有的原瑞星公司31．92％的股权价值，而刘旭也不得不再一次选择诉讼之路”（《“微点案”：没有赢家的恩怨情仇》，特别报道，2010年3月5日）。

乡镇初中英语教学问题初探

就我国乡镇初中教学现状，分析当前的乡镇中小学英语教学面临的主要问题，并结合笔者四十余年的中学英语教学经验，就此提出相应的解决对策。

对我市当前课堂教学改革中某些教学行为的反思

课改是提高基础教育教学质量的有效途径。从2010年开始，湖南省冷水江市开展了大规模的课堂教学改革，不断更新教学理念，课堂教学异彩纷呈。但笔者也发现，有些老师对课堂教学改革精神的理解有失偏颇，由此使课堂教学改革趋向简单化。在此，笔者试作分析，以期引起同行及教育行政部门的关注。

网络营销在崛起

“该专业团队之所以愿意主动承认‘贾君鹏’事件出自其之手，无非就是宣传自己，给更多有需求的客户提个醒：这种两天之内造就31万的跟帖回复，点击数达到760万的动静，对我们团队来说是‘小意思’，对你却可能是‘大意思’，只要肯花钱，多大的影响力我们都能给你创造出来”（《网托儿很近诚信很远》，“见解”，2009年9月20日）。

建设美丽城管从自己做起

当前，全国城管战线第一位的工作就是认真学习、深入贯彻党的十八大精神。作为城市管理者，我们的一言一行都密切关系到老百姓的利益，如何切实把党的十八大精神学懂学透，贯彻在工作中，落实在行动上，树立美丽城管形象，既是我们需要思考的问题，也是我们工作的追求目标。学习党的十八大精神，使我们深受振奋、凝心聚力、催人奋进。在今后的工作中，一定要以社会期望值、领导评价度和市民满意率作为衡量城管工作成效的标准，紧密联系工作实践，竭力解决民生、民难、民怨，坚决做到文明执法、亲民管理、热情服务，建设美丽城管必须从我们自己做起。