Objective The effects of Shuang Dan Ming Mu capsule on expression of VEGF-a,VEGF-b,VEGF-c and the VEGF receptor,Flk-1,were examined in a diabetic retinopathy rats model.Methods Forty Sprague-Dawley(SD)rats were random...Objective The effects of Shuang Dan Ming Mu capsule on expression of VEGF-a,VEGF-b,VEGF-c and the VEGF receptor,Flk-1,were examined in a diabetic retinopathy rats model.Methods Forty Sprague-Dawley(SD)rats were randomized into Groups A(blank),B(model),C(Shuang Dan Ming Mu)and D(positive control)group,with each group containing10rats and20eyes.Rats from groups B,C and D were administered one dose of50mg/kg streptozotocin(STZ)by tail vein injection to establish a diabetic rat model.One week after model preparation,medication was continuously administered by gavage.After gavage for8weeks,the animals were sacrificed and retinal expression of VEGF-a,VEGF-b,VEGF-c and Flk-1was quantified by immunohistochemical analysis.Results At week8of drug administration after model preparation,the average protein expression grayscale values for VEGF-a,VEGF-b,VEGF-c and Flk-1in the rats model,Shuang Dan Ming Mu and positive control groups were all lower than those in the normal group,while the mean optical density values were higher than those in the normal group.When the model group was compared to the normal group,the difference was extremely significant(P<0.01).The mean grayscale values of VEGF-a,VEGF-b,VEGF-c and Flk-1in the Shuang Dan Ming Mu and positive control groups were all higher than those in the model group,while the mean optical density values were lower than those in the model group(P<0.05or0.01).Conclusion Shuang Dan Ming Mu capsule can significantly decrease the expression of VEGF-a,VEGF-b,VEGF-c and Flk-1in the retinas of diabetic model rats and exhibit some protective effects in their retinas.展开更多
目的:观察青光安颗粒剂4种有效组分对兔眼滤过术后滤过道瘢痕组织TGF-β1和Smad3表达的影响,探讨青光安有效组分抗青光眼术后滤过道瘢痕化的效果及作用机制。方法:将青光安颗粒剂有效组分作用于滤过性手术后兔眼(有效组分1-4组),通过与...目的:观察青光安颗粒剂4种有效组分对兔眼滤过术后滤过道瘢痕组织TGF-β1和Smad3表达的影响,探讨青光安有效组分抗青光眼术后滤过道瘢痕化的效果及作用机制。方法:将青光安颗粒剂有效组分作用于滤过性手术后兔眼(有效组分1-4组),通过与正常组、模型组、丝裂霉素组(MMC组)的比较,观察青光安颗粒剂4种有效组分对青光眼术后滤过道瘢痕组织TGF-β1和Smad3的影响。结果:有效组分2组与MMC组TGF-β1和Smad3及m RNA表达比较差异无统计学意义,其余3组与MMC组TGF-β1和Smad3及m RNA表达比较,差异有显著性(P<0.05),其抑制效果不如MMC组。结论:青光安有效组分2和MMC都可通过抑制TGF-β1和Smad3 m RNA及其蛋白表达减少瘢痕组织增生,具有明显的抗青光眼术后滤过道瘢痕化的作用。展开更多
基金funding support from the National Natural Science Foundation General Program (No. 814737370)Hunan Province Graduate Student Research Innovation Program (No. CX2016B377 and No. CX2017B432)+4 种基金Traditional Chinese Medicine Prevention and Treatment of Facial Feature Disease Hunan Province Key Laboratory Construction Program (No. 2017TP1018)Changsha City Science and Technology Program (No. kc1704005)Central Finance Supported Local High School Construction ProjectState Administration of Traditional Chinese Medicine Ophthalmology Key Discipline Construction ProjectHunan Province Traditional Chinese Medicine Facial Feature Key Discipline Construction Project
文摘Objective The effects of Shuang Dan Ming Mu capsule on expression of VEGF-a,VEGF-b,VEGF-c and the VEGF receptor,Flk-1,were examined in a diabetic retinopathy rats model.Methods Forty Sprague-Dawley(SD)rats were randomized into Groups A(blank),B(model),C(Shuang Dan Ming Mu)and D(positive control)group,with each group containing10rats and20eyes.Rats from groups B,C and D were administered one dose of50mg/kg streptozotocin(STZ)by tail vein injection to establish a diabetic rat model.One week after model preparation,medication was continuously administered by gavage.After gavage for8weeks,the animals were sacrificed and retinal expression of VEGF-a,VEGF-b,VEGF-c and Flk-1was quantified by immunohistochemical analysis.Results At week8of drug administration after model preparation,the average protein expression grayscale values for VEGF-a,VEGF-b,VEGF-c and Flk-1in the rats model,Shuang Dan Ming Mu and positive control groups were all lower than those in the normal group,while the mean optical density values were higher than those in the normal group.When the model group was compared to the normal group,the difference was extremely significant(P<0.01).The mean grayscale values of VEGF-a,VEGF-b,VEGF-c and Flk-1in the Shuang Dan Ming Mu and positive control groups were all higher than those in the model group,while the mean optical density values were lower than those in the model group(P<0.05or0.01).Conclusion Shuang Dan Ming Mu capsule can significantly decrease the expression of VEGF-a,VEGF-b,VEGF-c and Flk-1in the retinas of diabetic model rats and exhibit some protective effects in their retinas.
文摘目的:观察青光安颗粒剂4种有效组分对兔眼滤过术后滤过道瘢痕组织TGF-β1和Smad3表达的影响,探讨青光安有效组分抗青光眼术后滤过道瘢痕化的效果及作用机制。方法:将青光安颗粒剂有效组分作用于滤过性手术后兔眼(有效组分1-4组),通过与正常组、模型组、丝裂霉素组(MMC组)的比较,观察青光安颗粒剂4种有效组分对青光眼术后滤过道瘢痕组织TGF-β1和Smad3的影响。结果:有效组分2组与MMC组TGF-β1和Smad3及m RNA表达比较差异无统计学意义,其余3组与MMC组TGF-β1和Smad3及m RNA表达比较,差异有显著性(P<0.05),其抑制效果不如MMC组。结论:青光安有效组分2和MMC都可通过抑制TGF-β1和Smad3 m RNA及其蛋白表达减少瘢痕组织增生,具有明显的抗青光眼术后滤过道瘢痕化的作用。
