湘产石菖蒲不同部位挥发性成分的GC-MS分析

目的:通过对石菖蒲不同部位的挥发油进行气相色谱-质谱法(GC-MS)分析,对比两个产地不同部位有效成分的含量。方法:采用水蒸气蒸馏法提取石菖蒲挥发油,利用GC-MS测定各样品中的各成分含量,根据样品处理条件进行各样品的相互比较。结果:分别从澧县新鲜石菖蒲根茎中提取出32种化合物,澧县干燥石菖蒲根茎中提取出31种化合物,长沙县新鲜石菖蒲根茎中提取出26种化合物,长沙县新鲜石菖蒲叶中提取出48种化合物。4个样品的挥发油主要成分均为α-细辛醚,其在澧县新鲜石菖蒲根茎挥发油中占96.31%,在澧县干燥石菖蒲根茎挥发油中占97.15%,在长沙县新鲜石菖蒲根茎挥发油中占81.60%,在长沙县新鲜石菖蒲叶挥发油中占60.08%。仅长沙县石菖蒲叶的挥发油中含β-细辛醚,占其挥发油的5.85%。结论:两个产地的石菖蒲根茎挥发油中含量都较高,且干燥和新鲜根茎中含量差异不明显,影响石菖蒲药效活性物质ɑ-细辛醚含量的主要因素有地理位置和不同部位。

Qualitative and Quantitative Analysis of Linoleic Acid in Polygonati Rhizoma

Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid determination.TLC chromatogram was improved significantly after optimization for qualitative analysis.Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest contents.This study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精).

气质联用法分析黄精种子油脂的脂肪酸成分

目的:分析研究黄精种子油脂的脂肪酸组分。方法:利用索氏提取法提取黄精种子的油脂,甲酯化后用气质联用法分析其油脂脂肪酸组成。结果:3种黄精种子平均粗脂肪相对含量分别为:多花黄精6.76%、滇黄精5.75%、黄精1.59%。经GC-MS分析,从多花黄精种子中提取的脂肪酸中共鉴定出45种化合物,其中主要以亚油酸含量最多,占总含量的51.56%;黄精种子脂肪酸中鉴定出75种化合物,主要成分为亚油酸,相对含量为47.02%;滇黄精种子脂肪酸中鉴定出54种化合物,主要成分为共轭亚油酸,相对含量为62.53%。结论:黄精种子油脂有较高的营养价值,可作为黄精种子综合开发利用的参考依据。