目的通过观察地高辛(Digoxin)干预血管紧张素Ⅱ(AngⅡ)诱导的ApoE-/-小鼠高血压性心肌肥厚模型后,对G蛋白调节因子2(regulator of G protein signaling 2,RGS2)的影响,探讨地高辛治疗高血压性心肌肥厚的作用与可能的机制。方法 30只雄性...目的通过观察地高辛(Digoxin)干预血管紧张素Ⅱ(AngⅡ)诱导的ApoE-/-小鼠高血压性心肌肥厚模型后,对G蛋白调节因子2(regulator of G protein signaling 2,RGS2)的影响,探讨地高辛治疗高血压性心肌肥厚的作用与可能的机制。方法 30只雄性ApoE-/-小鼠随机分为对照组、AngⅡ模型组和AngⅡ+Digoxin治疗组。所有小鼠从术前1d至术后处死前均接受0.5%二甲基亚砜或地高辛溶液腹腔注射治疗,并且在术后28d获取心脏组织,通过组织学检查、苏木精-伊红(HE)染色切片分析技术评定心肌组织形态学变化、RGS2的mRNA及蛋白表达水平来评价地高辛的作用。结果与AngⅡ模型组相比,AngⅡ+Digoxin组全心重量、左心室壁厚度、心肌细胞直径均明显减少(P<0.05或P<0.01),RGS2mRNA变化不明显,而蛋白表达增高(P<0.01),心肌肥厚明显减轻。同时检测小鼠在术前3d,手术当天,术后3、7、14、28d的血压,发现AngⅡ+Digoxin组与AngⅡ模型组比较,术后7d和14d血压下降(均P<0.05),术后28d则差异无统计学意义。结论地高辛可能通过上调ApoE-/-小鼠高血压心肌肥厚模型RGS2的表达,有效减轻心肌肥厚,这说明地高辛可能在抑制心肌细胞肥大中起重要作用。展开更多
目的探討腔鏡技術用於甲狀腺手術獲得美容效果的臨床應用價值及其安全性和微創性。方法2015年1月~2015年7月,行甲狀腺手術67例,其中腔鏡甲狀腺手術12例,傳統開放甲狀腺手術55例。比較兩種術式的手術時間、術中失血量、切口美容評分、術...目的探討腔鏡技術用於甲狀腺手術獲得美容效果的臨床應用價值及其安全性和微創性。方法2015年1月~2015年7月,行甲狀腺手術67例,其中腔鏡甲狀腺手術12例,傳統開放甲狀腺手術55例。比較兩種術式的手術時間、術中失血量、切口美容評分、術後住院時間、住院費用、手術併發症和復發率。結果兩組手術均獲成功,無出血、聲嘶、低血鈣等併發症。腔鏡組與開放組相比,失血量少(14±10m L vs 19±15m L,t=-2.270,P=0.023),切口美容滿意度高(VAS評分7.0±1.5 vs 2.0±1.4,t=24.549,P=0.000),術後頸前無手術疤痕,無皮膚色澤改變,但手術時間長(70±20min v s 60±30min,t=2.260,P=0.022),住院費用高(31843.5±897元vs 21859.4±683元,t=4.463,P=0.000)。發生切口積液腔鏡手術0例,開放組3例。隨訪1~6個月,平均4個月,復發2例,其中腔鏡組0例,傳統組2例。結論與傳統甲狀腺手術相比,腔鏡甲狀腺手術可獲得理想的美容效果,手術安全、恢復快,有着傳統開放甲狀腺手術不可比擬的優點,是治療良性甲狀腺疾病的理想手術方法。展开更多
瑪麗約瑟夫結節(sister Mary Joseph's nodule,SMJN)是指腹、盆腔内(主要是消化系統或泌尿生殖系統)惡性腫瘤轉移到臍部形成的腫瘤性結節。SMJN最早由在梅奧診所從事外科助理工作的瑪麗約瑟夫修女提出,她在日常護理工作中發現少數...瑪麗約瑟夫結節(sister Mary Joseph's nodule,SMJN)是指腹、盆腔内(主要是消化系統或泌尿生殖系統)惡性腫瘤轉移到臍部形成的腫瘤性結節。SMJN最早由在梅奧診所從事外科助理工作的瑪麗約瑟夫修女提出,她在日常護理工作中發現少數腹、盆腔惡性腫瘤患者有相似的臍部結節,並向外科醫生指出臍部結節可能是腹、盆腔惡性腫瘤轉移的體征之一。展开更多
Objective To investigate PPH of rectal nerve block anesthesia with the implementation of the anatomical basis and clinical application by verified.Methods Ischioanal fossas of 15 prostrated adult cadaveric specimens(3...Objective To investigate PPH of rectal nerve block anesthesia with the implementation of the anatomical basis and clinical application by verified.Methods Ischioanal fossas of 15 prostrated adult cadaveric specimens(30 s i d e s)were dissected to observe the distance and angle from anal nerves to anus.The adjacent structures of th anal nerves and it’s location point were also be measured.Application the clinical data of the method of location mark the implementation for 51 cases of PPH.Results The length from 3 o’clock or 9 o’clock position at anus to the trunk of anal nerves was about(4.93±0.91)cm(3.80~7.10cm).The angle of declination to the trunk from both positions was(15.6±3.3)°(15~20°).51 cases were operated in accordance with the implementation of the location mark to anal nerve block anesthesia,the patient can tolerate surgery stimulate,no additional intraoperative anesthetic and analgesic drugs,no anesthesia accidents,only 5 cases of urinary retention.Conclusion The position of anal nerves is quite invariable.The trunk of anal nerves locates in the outer 1/3 segment of the line of coccygeal tip to ischial tuberosity and is 2.21cm away from the medial region of ischial tuberosity,it is about 1.36cm in the front of the most bulgy aspect of the ischial tuberosity at the same level.This spot will be a much effective site for block anesthesia and can be implemented for the PPH.展开更多
文摘目的通过观察地高辛(Digoxin)干预血管紧张素Ⅱ(AngⅡ)诱导的ApoE-/-小鼠高血压性心肌肥厚模型后,对G蛋白调节因子2(regulator of G protein signaling 2,RGS2)的影响,探讨地高辛治疗高血压性心肌肥厚的作用与可能的机制。方法 30只雄性ApoE-/-小鼠随机分为对照组、AngⅡ模型组和AngⅡ+Digoxin治疗组。所有小鼠从术前1d至术后处死前均接受0.5%二甲基亚砜或地高辛溶液腹腔注射治疗,并且在术后28d获取心脏组织,通过组织学检查、苏木精-伊红(HE)染色切片分析技术评定心肌组织形态学变化、RGS2的mRNA及蛋白表达水平来评价地高辛的作用。结果与AngⅡ模型组相比,AngⅡ+Digoxin组全心重量、左心室壁厚度、心肌细胞直径均明显减少(P<0.05或P<0.01),RGS2mRNA变化不明显,而蛋白表达增高(P<0.01),心肌肥厚明显减轻。同时检测小鼠在术前3d,手术当天,术后3、7、14、28d的血压,发现AngⅡ+Digoxin组与AngⅡ模型组比较,术后7d和14d血压下降(均P<0.05),术后28d则差异无统计学意义。结论地高辛可能通过上调ApoE-/-小鼠高血压心肌肥厚模型RGS2的表达,有效减轻心肌肥厚,这说明地高辛可能在抑制心肌细胞肥大中起重要作用。
文摘目的探討腔鏡技術用於甲狀腺手術獲得美容效果的臨床應用價值及其安全性和微創性。方法2015年1月~2015年7月,行甲狀腺手術67例,其中腔鏡甲狀腺手術12例,傳統開放甲狀腺手術55例。比較兩種術式的手術時間、術中失血量、切口美容評分、術後住院時間、住院費用、手術併發症和復發率。結果兩組手術均獲成功,無出血、聲嘶、低血鈣等併發症。腔鏡組與開放組相比,失血量少(14±10m L vs 19±15m L,t=-2.270,P=0.023),切口美容滿意度高(VAS評分7.0±1.5 vs 2.0±1.4,t=24.549,P=0.000),術後頸前無手術疤痕,無皮膚色澤改變,但手術時間長(70±20min v s 60±30min,t=2.260,P=0.022),住院費用高(31843.5±897元vs 21859.4±683元,t=4.463,P=0.000)。發生切口積液腔鏡手術0例,開放組3例。隨訪1~6個月,平均4個月,復發2例,其中腔鏡組0例,傳統組2例。結論與傳統甲狀腺手術相比,腔鏡甲狀腺手術可獲得理想的美容效果,手術安全、恢復快,有着傳統開放甲狀腺手術不可比擬的優點,是治療良性甲狀腺疾病的理想手術方法。
文摘瑪麗約瑟夫結節(sister Mary Joseph's nodule,SMJN)是指腹、盆腔内(主要是消化系統或泌尿生殖系統)惡性腫瘤轉移到臍部形成的腫瘤性結節。SMJN最早由在梅奧診所從事外科助理工作的瑪麗約瑟夫修女提出,她在日常護理工作中發現少數腹、盆腔惡性腫瘤患者有相似的臍部結節,並向外科醫生指出臍部結節可能是腹、盆腔惡性腫瘤轉移的體征之一。
文摘Objective To investigate PPH of rectal nerve block anesthesia with the implementation of the anatomical basis and clinical application by verified.Methods Ischioanal fossas of 15 prostrated adult cadaveric specimens(30 s i d e s)were dissected to observe the distance and angle from anal nerves to anus.The adjacent structures of th anal nerves and it’s location point were also be measured.Application the clinical data of the method of location mark the implementation for 51 cases of PPH.Results The length from 3 o’clock or 9 o’clock position at anus to the trunk of anal nerves was about(4.93±0.91)cm(3.80~7.10cm).The angle of declination to the trunk from both positions was(15.6±3.3)°(15~20°).51 cases were operated in accordance with the implementation of the location mark to anal nerve block anesthesia,the patient can tolerate surgery stimulate,no additional intraoperative anesthetic and analgesic drugs,no anesthesia accidents,only 5 cases of urinary retention.Conclusion The position of anal nerves is quite invariable.The trunk of anal nerves locates in the outer 1/3 segment of the line of coccygeal tip to ischial tuberosity and is 2.21cm away from the medial region of ischial tuberosity,it is about 1.36cm in the front of the most bulgy aspect of the ischial tuberosity at the same level.This spot will be a much effective site for block anesthesia and can be implemented for the PPH.
