基因相关生物医学文献挖掘研究

系统生物学研究产生的大量生物医学文献包含了丰富的生物学知识。生物医学文献挖掘能够利用海量文献资源，获取国际上生命科学最新研究成果。我们开发了基因相关文献挖掘网络平台，应用自然语言处理技术，集成了文献自动采集工具、句法分析器、Gene Ontology等最新的生物医学领域知识库，能够对文献进行深度挖掘，进行基因功能、基因与疾病关系、生物分子相互作用网络知识发现．辅助形成生物科学研究创新假设，挖掘准确率可达86％。

An improved 3D shape haptic rendering algorithm for finger mounted vibrotactile device

To improve the sense of reality on perception, an improved algorithm of 3D shape haptic rendering is put forward based on a finger mounted vibrotactile device. The principle is that the interactive information and the shape information are conveyed to users when they touch virtual objects at mobile terminals by attaching the vibrotactile feedback on a fingertip. The extraction of shape characteristics, the interactive information and the mapping of shape in formation of vibration stimulation are key parts of the proposed algorithm to realize the real tactile rendering. The contact status of the interaction process, the height information and local gradient of the touch point are regarded as shape information and used to control the vibration intension, rhythm and distribution of the vibrators. With different contact status and shape information, the vibration pattern can be adjusted in time to imitate the outlines of virtual objects. Finally, the effectiveness of the algorithm is verified by shape perception experiments. The results show that the improved algorithm is effective for 3D shape haptic rendering.

Population Change of OH and H2O in Water Vapor Glow Discharge Measured Using Concentration Modulation Spectroscopy

A distributed feedback laser with a wavelength of 2.8μm was used to measure the species produced by water vapor glow discharge.Only the absorption spectra of OH radicals and transient H2O molecules were observed using concentration modulation(CM)spectroscopy.The intensities and orientations of the absorption peaks change with the demodulation phase,but the direction of one absorption peak of H2O is always opposite to the other peaks.The different spectral orientations of OH and H2O reflect the increase or the decrease of the number of particles in the energy levels.If more transient species can be detected in the discharge process,the dynamics of excitation,ionization,and decomposition of H2O can be better studied.This study shows that the demodulation phase relationship of CM spectrum can be used to study the population change of molecular energy levels.

Evolutionary selection on synonymous codons in RNA G-quadruplex structural region

To investigate how synonymous codons have been adapted to the formation of ribonucleic acid(RNA)G-quadruplex(rG4)structure,a computational searching algorithm G4Hunter was applied to detect rG4 structures in protein-coding sequences of mRNAs in five eukaryotic species.The native sequences forming rG4s were then compared with randomized sequences to evaluate selection on synonymous codons.Factors that may influence the formation of rG4 were also investigated,and the selection pressures of rG4 in different gene regions were compared to explore its potential roles in gene regulation.The results show universal selective pressure acts on synonymous codons in rG4 regions to facilitate rG4 formation in five eukaryotic organisms.While G-rich codon combinations are preferred in the rG4 structural region,C-rich codon combinations are selectively unfavorable for rG4 formation.Gene's codon usage bias,nucleotide composition,and evolutionary rate can account for the selective variations on synonymous codons among rG4 structures within a species.Moreover,rG4 structures in the translational initiation region showed significantly higher selective pressures than those in the translational elongation region.

Sum Frequency Spectroscopy Studies on Cell Membrane Fusion Induced by Divalent Cations

Cell membrane fusion is a fundamental biological process involved in a number of cellular living functions. Regarding this, divalent cations can induce fusion of the lipid bilayers through binding and bridging of divalent cations to the charged lipids, thus leading to the cell membrane fusion. However, the elaborate mechanism of cell membrane fusion induced by divalent cations is still needed to be elucidated.Here, surface/interface sensitive sum frequency generation vibrational spectroscopy(SFG-VS) and dynamic light scattering(DLS) were applied in this research to study the responses of phospholipid monolayer to the exposure of divalent metal ions i.e.Ca^(2+)and Mg^(2+). According to the particle size distribution results measured by DLS experiments, it was found that Ca^(2+)could induce inter-vesicular fusion while Mg^(2+)could not. An octadecyltrichlorosilane self-assembled monolayer(OTS SAM)-lipid monolayer system was designed to model the cell membrane for the SFG-VS experiment. Ca^(2+)could interact with the lipid POO_(2)^(-)head groups more strongly, resulting in cell membrane fusion more easily, in comparison with Mg^(2+). No specific interaction between the two metal cations and the C=O groups was observed. However, the C=O orientations changed more after Ca^(2+)-PO2-binding than Mg^(2+)mediation on lipid monolayer. Meanwhile, Ca^(2+)could induce dehydration of the lipids(which should be related to the strong Ca^(2+)-PO_(2)^(-)interaction), leading to the reduced hindrance for cell membrane fusion.

Intracellular temperature measurement by a dual-thermocouple difference method

A dual thermocouple difference technique is developed to determine the accuracy and anti-interference ability in the process of intracellular temperature measurement.First,two micro-nano thermocouples(TC)and a high-precision signal acquisition module are used to measure the temperature difference between the cell and the culture medium(separated about 10μm from the cell).The cold junctions of two TCs are connected to eliminate the setting of the reference temperature and enhance the anti-interference ability.Then,a low-noise voltage amplifier and digital acquisition card are used to sample signals.In order to verify the feasibility of the dual thermocouple difference method,the temperature changes of U251 cells are detected.The calibration results of two TCs show that the Seebeck coefficient is about 5μV/℃,and the signal acquisition accuracy is 0.5μV in a low voltage range(0-15μV).With the dual thermocouple difference method,errors due to the cold junctions can be removed and the interference caused by environmental temperature fluctuation can be reduced.The phenomenon of cellular temperature increase proves that the dual thermocouple difference method can detect the tiny temperature change of a single cell.The method potentially is a highly powerful technique for studying local thermogenesis of cells and helps to explore the relationship between cellular thermogenesis and cellular processes.

Amide-linked covalent organic frameworks as efficient heterogeneous photocatalysts in water

Semiconductor photocatalysts play an indispensable role in the photocatalytic process.Two-dimensional covalent organic frameworks(2D-COFs),as a kind of innovative photocatalyst,have garnered tremendous attention.Herein,we report an amide-linked 2D-COF(COF-JLU19)with outstanding photocatalytic performance in water,designed through a multi-synergistic approach.The synergistic effects of the high porosity,photoactive framework,high wettability,and stability of COF-JLU19 led to an unprecedented enhancement in the photocatalytic activity and recyclability in water upon illumination by visible light.More importantly,amide-linked 2D-COF based electrospinning membranes were prepared,which also exhibited superior photocatalytic activity for the degradation of Rhodamine B in water with sunlight.This study highlights the potential of the multi-synergistic approach as a universal rule for developing COF-based photocatalysts to address environmental and energy challenges.

Effect of Defects on Magnetic Properties of O^(+) -implanted AlN Films by Positron Annihilation Spectroscopy

Room-temperature ferromagnetism is observed in the O^(+)-implanted AIN films with O+doses of 5×10^(16) cm^(-2)(AIN:Osxion)and 2×10^(17) cm^(-2)(AIN:O_(2x1on)).The observed magnetic anisotropy indicates that the ferromagnetism is attributed to the intrinsic properties of O^(+) -implanted AIN films.The out-of-plane saturation magnetization(Ms)of the AIN:O5×10^(16) is about 0.68 emu/g,much higher than that of AIN:O_(2x1017),0.09 emu/g,which is due to the excessively high O^(+)dose made more O+ions occupy adjacent A1^(3+)positions in forms of antiferromagnetic coupling.Doppler broadening of positron annihilation radiation measurements demonstrate the existence of Al vacancies in the O^(+)-implanted AIN films.The first-principles calculations suggest that the ferromagnetism originates mainly from the Al vacancies.Meanwhile,the formation of divacancies or vacancy clusters by high concentrations of Al vacancies will lead to the transformation of VA-VAi coupling from ferromagnetim to antiferromagnetism,ultimately weakening the ferromagnetism of the sample.

汉坦病毒感染的量子点标记免疫检测研究

目的 应用量子点荧光标记技术和间接免疫法,建立一种新的汉坦病毒（HV）感染的免疫检测技术.方法 采用碳二亚胺交联法,在水溶性量子点表面修饰protein G和羊抗人IgG.以HV抗原片为固相载体,QDs-PG-IgG复合物为标记二抗,检测待检血清中的HV特异性抗体,并对检测条件进行优化.结果 量子点与羊抗人IgG的最佳偶联条件：反应时间2h、pH 6.0、羊抗人IgG浓度20 μg/ml.检测体系的最佳反应条件：量子点标记二抗的最适工作浓度为1：200,HV感染阳性血清检测的最大稀释度为1：1280.结论 成功建立了简便、快速的HV感染的量子点标记免疫检测方法,该方法具有良好的灵敏度和特异性,抗荧光猝灭性强,为进一步实现HV抗体的单分子定量检测奠定基础.