Effects of Different Extract of Pseudostellaria Heterophylla on Immunological Function in Mice based on Meta-analysis and Network Meta-analysis

To evaluate the effects of different extract of Pseudostellaria heterophylla on immunological function in mice based on Meta-analysis and Network meta-analysis,the article retrieved domestic and foreign databases according to the"PICO"retrieval strategy,and used Stata and ADDIS software for Meta analysis.A total of 6 reports,10 randomised controlled trials(RCTs)were eventually involved.Meta analysis results showed that:compared with the control group,the experimental group of polysaccharide,saponin and crude extract were better than that of the control(P<0.05),which significantly improved the immunological function in mice.Network meta-analysis results showed that the saponin had the best effect on the increase of phagocytic index and the differences were statistically significant[MD=1.50,95%CI(0.71,2.28),P<0.05];The polysaccharide and saponin had better effect on the increase of spleen index than the control and crude extract,and the differences were statistically significant[MD=0.77,95%CI(0.27,1.31),P<0.05],[MD=0.71,95%CI(0.01,1.50),P<0.05];the polysaccharide had the best effect on the increase of thymus index and the differences were statistically significant[MD=0.70,95%CI(0.11,1.34),P<0.05].The rank probability showed that the saponin of Pseudostellaria heterophylla had the maximum probability to increase phagocytic index of mice;the probability for the components of Pseudostellaria heterophylla increasing spleen index of mice was in the order of crude extract>polysaccharide>saponin;the probability for the components of Pseudostellaria heterophylla increasing thymus index of mice was in the order of saponin>polysaccharide>crude extract.Based on the available evidence,the extract of Pseudostellaria heterophylla could improve the immunity of mice,and the clinical effect of polysaccharide and saponin was the best,which provided a more valuable scientific reference for evidencing that the polysaccharide and saponin of Pseudostellaria heterophylla was the effective components for improving immunological function,and also was conducive to the proper further development of Pseudostellaria heterophylla resources.

中药复方对猪蓝耳病阳性场母猪繁殖性能及血液生理生化指标的影响

为探讨中药对猪蓝耳病阳性场母猪繁殖性能及血液生理生化指标的影响,试验随机选取20头胎次相近、妊娠90 d的长大二元母猪,随机分成0.1%、0.3%、0.5%中药组和空白对照组,试验期20 d,停药当日早上空腹采血当天检测外周血血液免疫细胞数及生化指标,考察死胎数、弱仔数、木乃伊胎数等繁殖指标。结果表明:与空白对照组比较,0.5%和0.3%中药组死胎数显著降低(P<0.05);0.3%中药组弱仔数显著降低(P<0.05);0.3%中药组淋巴细胞数提高53.6%(P<0.05);0.3%中药组总蛋白含量提高6.9%(P<0.05);0.1%和0.3%中药组球蛋白含量分别提高22.3%(P<0.05)和15.1%(P>0.05),0.5%中药组降低9.1%(P>0.05),0.5%中药组显著低于0.3%中药组(P<0.05),极显著低于0.1%中药组(P<0.01);0.3%中药组丙氨酸氨基转移酶活性显著降低(P<0.05);0.1%和0.3%中药组的血糖含量分别降低了19.3%(P<0.05)、14.9%(P<0.05);0.1%中药组的总胆红素含量显著降低(P<0.05)。说明自拟中药复方能降低猪蓝耳病阳性场母猪产弱仔及死胎数,提高母猪繁殖性能;提高母猪外周血免疫细胞数量,调节免疫功能;改善母猪血浆生化指标,改善肝脏功能。0.3%中药组效果最好。

基于gyrB基因建立快速检测沙雷菌的PCR方法

选择gyr B基因作为靶基因设计特异性引物用于沙雷菌属的PCR检测,该特异性引物扩增产物的片段大小为279 bp。研究所选用的14株沙雷菌分别代表沙雷菌属的7个不同种。此外,2种沙门菌、2种克雷伯菌以及其他5种肠道菌用于进行引物特异性的验证。结果表明:扩增的条带与预期的扩增产物片段大小一致,而非沙雷菌属的其他9个种属均没有扩增条带;该方法检测沙雷菌的最低检测限为9.785 pg。通过对4株从蜜蜂中分离所得的黏质沙雷菌和3株从进口鱼粉中分离所得的居泉沙雷菌进行检测,结果均为阳性。研究表明,基于gyr B基因建立的PCR方法在沙雷菌属的检测中具有特异性强、快速和灵敏度高等特点。