目的观察腹腔注射盐酸氨溴索对气管内注射阿米卡星后大鼠气管黏膜损伤的超微结构的影响。方法将30只Wistar大鼠经气管穿刺注入阿米卡星,随机分为对照组(阿米卡星组,15只)及氨溴索干预组(15只),经大鼠腹腔注射盐酸氨溴索注射液,在2、4、8...目的观察腹腔注射盐酸氨溴索对气管内注射阿米卡星后大鼠气管黏膜损伤的超微结构的影响。方法将30只Wistar大鼠经气管穿刺注入阿米卡星,随机分为对照组(阿米卡星组,15只)及氨溴索干预组(15只),经大鼠腹腔注射盐酸氨溴索注射液,在2、4、8、24及48 h 5个时间点处死大鼠各3只。取气管下1/3段的腹侧部,采用扫描电镜观察气道表面结构。结果阿米卡星组均出现不同程度的黏液分泌增加,黏稠度增加,纤毛倒伏、粘着在一起、排列紊乱、部分断裂缺失,恢复较慢,2、4、8、24及48 h受损面积百分比分别为98.2%、98.5%、97.5%、92.7%及82.1%。氨溴索干预组上述改变较轻,修复较快,2、4、8、24及48 h受损面积百分比分别为85.7%、81.9%、73.0%、61.9%及50.2%,恢复明显较对照组快。经气管注药在各时间点阿米卡星组纤毛受损面积明显高于氨溴索干预组(P<0.01)。结论阿米卡星可对大鼠气道黏膜表面结构造成严重损害,氨溴索可促进气管黏膜的修复,氨溴索注射液法治疗气管损伤效果理想,值得临床推广使用。展开更多
目的 研究强直性脊柱炎(as)患者体内血液循环和关节腔内肺炎衣原体感染情况,寻找肺炎衣原体导致as的临床证据.方法 收集33例as患者和22名健康献血者的外周血,酶联免疫法(elisa)检测血清中igg/igm抗体,免疫荧光法(ifa)和聚合酶链反应(pcr...目的 研究强直性脊柱炎(as)患者体内血液循环和关节腔内肺炎衣原体感染情况,寻找肺炎衣原体导致as的临床证据.方法 收集33例as患者和22名健康献血者的外周血,酶联免疫法(elisa)检测血清中igg/igm抗体,免疫荧光法(ifa)和聚合酶链反应(pcr)检测外周血单个核细胞中衣原体脂多糖(lps)抗原和肺炎衣原体核酸,同时收集患者的临床资料.另采用免疫组织化学法检测9例髋关节置换的as患者和13例骨折患者滑膜中的衣原体lps抗原.结果 as患者外周血肺炎衣原体igm、lps和pcr的阳性率均显著高于健康献血者(分别为78.8％vs.22.7％,x2=16.867,p=0.000;66.7％vs.31.8％,x2 =6.431,p=0.011;33.3％vs.9.1％,x2 =4.298,p=0.038).肺炎衣原体pcr阳性与红细胞沉降率(esr)和c反应蛋白(crp)水平相关(z=-2.774 和-2.829,p值均为0.004).77.8％ (7/9)的as患者的滑膜中可检测到含有衣原体lps抗原的炎性细胞,而骨折患者滑膜中仅30.8％ (4/13)病例中检测到含有衣原体lps抗原的炎性细胞(p=0.08).结论 肺炎衣原体在as患者外周血和滑膜中有很高的感染率,肺炎衣原体感染可能与as 发病相关.
abstract：
objective to investigate the association of chlamydia pneumoniae infection with ankylosing spondylitis (as).methods serum samples were obtained from 33 as patients and 22 healthy controls.enzyme-linked immunosorbent assay (elisa) was applied to mearsure serum anti-chlamydia pneumoniae antibodies (igm/igg),while immunofluorescence assay (ifa) was used to detect chlamydia pneumoniae lps antigen,and polymerase chain reaction (pcr) was used to amplify chlamydia pneumoniae dna in peripheral blood cells. immunohistochemistical technique was applied to examine chlamydia pneumoniae lps antigen in synovial tissue from another 9 as patients who received total hip replacement and 13 patients with comminuted femoral fractures.results the positive rates of chlamydia pneumoniae igm,lps antigen and chlamydia pneumoniae dna were higher in as patients than those in healthy controls (78.8％ vs 22.7％,x2 =16.867,p =0.000; 66.7％ vs 31.8％,x2 =6.431,p =0.011; 33.3％ vs 9.1％,x2 =4.298,p =0.038).chlamydia pneumoniae dna positive rate was correlated with erythrocyte sedimentation rate (esr) and c-reactive protein (crp) levels (z =-2.774 and -2.829,p =0.004).in synovial tissues,chlamydial lps-containing inflammatory cells were observed in 77.8％ (7/9) as patients,while those in fracture patients was 30.8％ ( 4/13 ) ( p =0.08 ).conclusion chlamydia pneumoniae infection is common in blood circulation and joint cavity of as patients and may be associated with the pathogenesis of as.展开更多
文摘目的观察腹腔注射盐酸氨溴索对气管内注射阿米卡星后大鼠气管黏膜损伤的超微结构的影响。方法将30只Wistar大鼠经气管穿刺注入阿米卡星,随机分为对照组(阿米卡星组,15只)及氨溴索干预组(15只),经大鼠腹腔注射盐酸氨溴索注射液,在2、4、8、24及48 h 5个时间点处死大鼠各3只。取气管下1/3段的腹侧部,采用扫描电镜观察气道表面结构。结果阿米卡星组均出现不同程度的黏液分泌增加,黏稠度增加,纤毛倒伏、粘着在一起、排列紊乱、部分断裂缺失,恢复较慢,2、4、8、24及48 h受损面积百分比分别为98.2%、98.5%、97.5%、92.7%及82.1%。氨溴索干预组上述改变较轻,修复较快,2、4、8、24及48 h受损面积百分比分别为85.7%、81.9%、73.0%、61.9%及50.2%,恢复明显较对照组快。经气管注药在各时间点阿米卡星组纤毛受损面积明显高于氨溴索干预组(P<0.01)。结论阿米卡星可对大鼠气道黏膜表面结构造成严重损害,氨溴索可促进气管黏膜的修复,氨溴索注射液法治疗气管损伤效果理想,值得临床推广使用。
文摘目的 研究强直性脊柱炎(as)患者体内血液循环和关节腔内肺炎衣原体感染情况,寻找肺炎衣原体导致as的临床证据.方法 收集33例as患者和22名健康献血者的外周血,酶联免疫法(elisa)检测血清中igg/igm抗体,免疫荧光法(ifa)和聚合酶链反应(pcr)检测外周血单个核细胞中衣原体脂多糖(lps)抗原和肺炎衣原体核酸,同时收集患者的临床资料.另采用免疫组织化学法检测9例髋关节置换的as患者和13例骨折患者滑膜中的衣原体lps抗原.结果 as患者外周血肺炎衣原体igm、lps和pcr的阳性率均显著高于健康献血者(分别为78.8％vs.22.7％,x2=16.867,p=0.000;66.7％vs.31.8％,x2 =6.431,p=0.011;33.3％vs.9.1％,x2 =4.298,p=0.038).肺炎衣原体pcr阳性与红细胞沉降率(esr)和c反应蛋白(crp)水平相关(z=-2.774 和-2.829,p值均为0.004).77.8％ (7/9)的as患者的滑膜中可检测到含有衣原体lps抗原的炎性细胞,而骨折患者滑膜中仅30.8％ (4/13)病例中检测到含有衣原体lps抗原的炎性细胞(p=0.08).结论 肺炎衣原体在as患者外周血和滑膜中有很高的感染率,肺炎衣原体感染可能与as 发病相关.
abstract：
objective to investigate the association of chlamydia pneumoniae infection with ankylosing spondylitis (as).methods serum samples were obtained from 33 as patients and 22 healthy controls.enzyme-linked immunosorbent assay (elisa) was applied to mearsure serum anti-chlamydia pneumoniae antibodies (igm/igg),while immunofluorescence assay (ifa) was used to detect chlamydia pneumoniae lps antigen,and polymerase chain reaction (pcr) was used to amplify chlamydia pneumoniae dna in peripheral blood cells. immunohistochemistical technique was applied to examine chlamydia pneumoniae lps antigen in synovial tissue from another 9 as patients who received total hip replacement and 13 patients with comminuted femoral fractures.results the positive rates of chlamydia pneumoniae igm,lps antigen and chlamydia pneumoniae dna were higher in as patients than those in healthy controls (78.8％ vs 22.7％,x2 =16.867,p =0.000; 66.7％ vs 31.8％,x2 =6.431,p =0.011; 33.3％ vs 9.1％,x2 =4.298,p =0.038).chlamydia pneumoniae dna positive rate was correlated with erythrocyte sedimentation rate (esr) and c-reactive protein (crp) levels (z =-2.774 and -2.829,p =0.004).in synovial tissues,chlamydial lps-containing inflammatory cells were observed in 77.8％ (7/9) as patients,while those in fracture patients was 30.8％ ( 4/13 ) ( p =0.08 ).conclusion chlamydia pneumoniae infection is common in blood circulation and joint cavity of as patients and may be associated with the pathogenesis of as.
